UBE2C (NM_181801) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC200240. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 15.6 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Ubiquitin mediated proteolysis

Protein Data

RefSeq: NP_861517	RefSeq Size: 936	RefSeq ORF: 423
LocusID: 11065	Cytogenetic: 20q13.12
Synonyms : dJ447F3.2; UBCH10
Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens ubiquitin-conjugating enzyme E2C (UBE2C), transcript variant 4 as transfection-ready DNA • Lenti ORF clone of Human ubiquitin-conjugating enzyme E2C (UBE2C), transcript variant 4, mGFP tagged • Lenti ORF clone of Human ubiquitin-conjugating enzyme E2C (UBE2C), transcript variant 4 , Myc-DDK-tagged • GFP-tagged ORF clone of Homo sapiens ubiquitin-conjugating enzyme E2C (UBE2C), transcript variant 4 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens ubiquitin-conjugating enzyme E2C (UBE2C), transcript variant 4 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of ubiquitin-conjugating enzyme E2C (UBE2C), transcript variant 4 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • UBE2C - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • UBE2C - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Ube2c - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • UBE2C - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Ube2c - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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