ADI1 (NM_018269) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC200115. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 21.3 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways: Cysteine and methionine metabolismMetabolic pathways

Protein Data

RefSeq: NP_060739	RefSeq Size: 1628	RefSeq ORF: 540
LocusID: 55256	Cytogenetic: 2p25.3
Synonyms : APL1; ARD; Fe-ARD; HMFT1638; MTCBP1; Ni-ARD; SIPL
Summary: This gene encodes an enzyme that belongs to the aci-reductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. This enzyme may regulate mRNA processing in the nucleus, and may carry out different functions depending on its localization. Related pseudogenes have been defined on chromosomes 8 and 20. [provided by RefSeq, May 2010].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens acireductone dioxygenase 1 (ADI1) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens acireductone dioxygenase 1 (ADI1) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens acireductone dioxygenase 1 (ADI1) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of acireductone dioxygenase 1 (ADI1) Validated Antibodies • Purified anti-ADI1 mouse monoclonal antibody, clone 3H6 • Purified ADI1 mouse monoclonal antibody, clone 5C2 • Carrier-free (BSA/glycerol-free) ADI1 mouse monoclonal antibody, clone 3H6 • Carrier-free (BSA/glycerol-free) ADI1 mouse monoclonal antibody, clone 5C2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • ADI1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • ADI1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Adi1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • ADI1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Adi1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene ADI1

If you would like OriGene Technologies to prepare a purified protein in the quantity of your own interest, simply fill out the form and click Submit. OriGene will contact you.

Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
* Quantity (µg)
Comments
* First Name
* Last Name
* Email Address
* Institution Name
* Country
* Phone