GIT2 (NM_139201) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC200045. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 52.4 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Endocytosis

Protein Data

RefSeq: NP_631940	RefSeq Size: 2264	RefSeq ORF: 1416
LocusID: 9815	Cytogenetic: 12q24.1
Synonyms : CAT-2; CAT2
Summary: This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq, Sep 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 4 as transfection-ready DNA • Lenti ORF clone of Human G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 4 , Myc-DDK-tagged • Lenti ORF clone of Human G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 4, mGFP tagged • GFP-tagged ORF clone of Homo sapiens G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 4 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 4 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 4 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • GIT2 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • GIT2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Git2 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • GIT2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Git2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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