|Expression cDNA Clone and Sequence
Recombinant protein was produced with TrueORF clone, RC200003. Click on the TrueORF clone link to view cDNA and protein sequences.|
||Predicted MW:||43.5 kDa|
|Purity:||> 80% as determined by SDS-PAGE and Coomassie blue staining|
|Concentration:||>50 ug/mL as determined by microplate BCA method|
|Buffer and Storage:||25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.|
||Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
||TP53 Activity Verified in a DNA-binding Assay: TP53 (TP304649) activity was measured in a colorimetric DNA-binding assay. Double-stranded oligonucleotide containing the p53 consensus DNA-binding sequence was incubated with dilutions of the purified TP53 protein and TP53 bound to the oligo was captured onto the surface of a microtiter plate. After washing, bound TP53 was detected with an anti-p53 primary antibody followed by an HRP-labeled secondary antibody. After initial color development, the reaction was quenched and the color intensity was measured at 450nm.
||Stem cell - PluripotencyTranscription FactorsDruggable Genome
||MAPK signaling pathwayCell cyclep53 signaling pathwayApoptosisWnt signaling pathwayNeurotrophin signaling pathwayMore Pathways >>