Akt1 (BC066018) Mouse Untagged Clone

CAT#: MC206055

Akt1 (untagged) - Mouse thymoma viral proto-oncogene 1 (cDNA clone MGC:90109 IMAGE:5716837), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Akt1
• Synonyms: Akt, PKB/Akt, PKBalpha, PKB
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC066018
  GCGGGGCGGGGAGAAGGCGGGCCGGCGGCGGCGGCGGCAGCACCGAGTCGGCGGGCGGCCGGCCCAGCGC GGCAGCGCACGCGAGTCCGGGACCAGCGGAGCGGACCGAGCAGCGTCCTGTGGCCGGCACCGCGGCGGCC CAGATCCGGCCAGCAGCGCGCGCCCGGACGCCGCTGCCTTCAGCCGGCCCCGCCCAGCGCCCGCCCGCGG GATGCGGAGCGGCGGGCGCCCGAGGCCGCGGCCCGGCTAGGCCCAGTCGCCCGCACGCGGCGGCCCGACG CTGCGGCCAGGCCGGCTGGGCTCAGCCTACCGAGAAGAGACTCTGAGCATCATCCCTGGGTTACCCCTGT CTCTGGGGGCCACGGATACCATGAACGACGTAGCCATTGTGAAGGAGGGCTGGCTGCACAAACGAGGGGA ATATATTAAAACCTGGCGGCCACGCTACTTCCTCCTCAAGAACGATGGCACCTTTATTGGCTACAAGGAA CGGCCTCAGGATGTGGATCAGCGAGAGTCCCCACTCAACAACTTCTCAGTGGCACAATGCCAGCTGATGA AGACAGAGCGGCCAAGGCCCAACACCTTTATCATCCGCTGCCTGCAGTGGACCACAGTCATTGAGCGCAC CTTCCATGTGGAAACGCCTGAGGAGCGGGAAGAATGGGCCACCGCCATTCAGACTGTGGCAGATGGACTC AAGAGGCAGGAAGAAGAGACGATGGACTTCCGATCAGGCTCACCCAGTGACAACTCAGGGGCTGAAGAGA TGGAGGTGTCCCTGGCCAAGCCCAAGCACCGTGTGACCATGAACGAGTTTGAGTACCTGAAGCTACTGGG CAAGGGCACCTTTGGGAAGGTGATTCTGGTGAAAGAGAAGGCCACAGGCCGCTACTATGCCATGAAGATC CTCAAGAAGGAGGTCATCGTCGCCAAGGATGAGGTTGCCCACACGCTTACTGAGAACCGTGTCCTGCAGA ACTCTAGGCATCCCTTCCTTACGGCCCTCAAGTACTCATTCCAGACCCACGACCGCCTCTGCTTTGTCAT GGAGTATGCCAACGGGGGCGAGCTCTTCTTCCACCTGTCTCGAGAGCGTGTGTTCTCCGAGGACCGGGCC CGCTTCTATGGTGCGGAGATTGTGTCTGCCCTGGACTACTTGCACTCCGAGAAGAACGTGGTGTACCGGG ACCTGAAGCTGGAGAACCTCATGCTGGACAAGGACGGGCACATCAAGATAACGGACTTCGGGCTGTGCAA GGAGGGGATCAAGGACGGTGCCACTATGAAGACATTCTGCGGAACGCCGGAGTACCTGGCCCCTGAGGTG CTGGAGGACAACGACTACGGCCGTGCAGTGGACTGGTGGGGGCTGGGCGTGGTCATGTACGAGATGATGT GTGGCCGCCTGCCCTTCTACAACCAGGACCACGAGAAGCTGTTCGAGCTGATCCTCATGGAGGAGATCCG CTTCCCGCGCACACTCGGCCCTGAGGCCAAGTCCCTGCTCTCCGGGCTGCTCAAGAAGGACCCTACACAG AGGCTCGGTGGGGGCTCCGAGGATGCCAAGGAGATCATGCAGCACCGGTTCTTTGCCAACATCGTGTGGC AGGATGTGTATGAGAAGAAGCTGAGCCCACCTTTCAAGCCCCAGGTCACCTCTGAGACTGACACCAGGTA TTTCGATGAGGAGTTCACAGCTCAGATGATCACCATCACGCCGCCTGATCAAGATGACAGCATGGAGTGT GTGGACAGTGAGCGGAGGCCGCACTTCCCCCAGTTCTCCTACTCAGCCAGTGGCACAGCCTGAGGCCTGG GGCAGCGGCTGGCAGCTCCACGCTCCTCTGCATTGCCGAGTCCAGAAGCCCCGCATGGATCATCTGAACC TGATGTTTTGTTTCTCGGATGCGCTGGGGAGGAACCTTGCCAGCCTCCAGGACCAGGGGAGGATGTTTCT ACTGTGGGCAGCAGCCTACCTCCCAGCCAGGTCAGGAGGAAAACTATCCTGGGGTTTTTCTTAATTTATT TCATCCAGTTTGAGACCACACATGTGGCCTCAGTGCCCAGAACAATTAGATTCATGTAGAAAACTATTAA GGACTGACGCGACCATGTGCAATGTGGGCTCATGGGTCTGGGTGGGTCCCGTCACTGCCCCCATTGGCCT GTCCACCCTGGCCGCCACCTGTCTCTAGGGTCCAGGGCCAAAGTCCAGCAAGAAGGCACCAGAAGCACCC CCCTGTGGTATGCTAACTGGCCCTCTCCCTCTGGGCGGGGAGAGGTCACAGCTGCTTCAGCCCTAGGGCT GGATGGGATGGCCAGGGCTCAAGTGAGGTTGACAGAGGAACAAGAATCCAGTTTGTTGCTGTGTCCCATG CTGTTCAGAGACATTTAGGGGATTTTAATCTTGGTGACAGGAGAGCCCCTGCCCTCCCGCACCCGCTCCC GCGTGGTGGCTCTTAGCGGGTACCCTGGGAGCGCCTGCCTCACGTGAGCCCTTCTCCTAGCACTTGTCCT TTTAGATGCTTTCCCTCTCCCGCTGTCCGTCACCCTGGCCTGTCCCCTCCCGGCCAGACGCTGGCCATTG CTGCACCATGTCGTTTTTTACAACATTCAGCTTCAGCATTTTTACTATTATAATAAGAAACTGTCCCTCC AAATTCAATAAAAATTGCTTTTCAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC066018
• Insert Size: 1443 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC066018, AAH66018
• RefSeq Size: 2701 bp
• RefSeq ORF: 1443 bp
• Locus ID: 11651
• Cytogenetics: 12 61.2 cM
• Gene Summary: This gene encodes the founding member of the Akt serine-threonine protein kinase gene family that also includes Akt2 and Akt3. This kinase is a major downstream effector of the phosphatidylinositol 3-kinase (PI3K) pathway that mediates the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). It is activated through recruitment to cellular membranes by PI3K lipid products and by phosphorylation by 3-phosphoinositide dependent kinase-1. It then further phosphorylates different downstream proteins in response to various extracellular signals and thus plays a pivotal role in mediating a variety of cellular processes, such as glucose metabolism, glycogen biosynthesis, protein synthesis and turn over, inflammatory response, cell survival (anti-apoptosis) and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]