Erbb4 (NM_010154) Mouse Tagged ORF Clone

CAT#: MR225933

Erbb4 (Myc-DDK-tagged) - Mouse v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) (Erbb4)

Product Images

Circular map for MR225933

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Erbb4
• Synonyms: c-erbB-4; Her4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225933 representing NM_010154
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTGGCGACGGGACTCTGGGTCTGGGGGAGCCTTCTGATGGCAGCGGGGACCGTCCAGCCCAGCG
  CTTCTCAGTCAGTGTGCGCAGGAACAGAGAACAAACTGAGCTCTCTCTCTGACCTGGAACAGCAGTACCG
  AGCCTTGCGCAAATACTATGAAAACTGCGAGGTAGTCATGGGCAACCTGGAGATCACCAGCATCGAGCAC
  AACCGGGACCTCTCCTTCCTGCGGTCTATCCGAGAAGTCACAGGCTACGTCCTGGTGGCCCTCAACCAGT
  TTCGTTACTTGCCTCTGGAGAATTTACGCATTATTCGTGGGACAAAACTATATGAAGATCGCTATGCCTT
  AGCGATATTCTTAAACTACAGGAAAGATGGCAACTTTGGACTCCAAGAACTTGGATTAAAGAACCTGACC
  GAAATACTAAATGGTGGAGTCTATGTAGACCAGAACAAATTCCTATGTTATGCTGACACTATACACTGGC
  AAGATATTGTTCGGAATCCATGGCCTTCCAACATGACTCTGGTGTCAACAAATGGAAGTTCTGGATGTGG
  AAGATGCCATAAGTCTTGCACTGGCCGATGCTGGGGACCCACAGAAAATCACTGCCAGACCTTGACCAGA
  ACTGTGTGTGCTGAACAATGTGATGGCAGGTGCTATGGACCCTACGTTAGTGACTGCTGCCATCGAGAAT
  GTGCTGGAGGCTGCTCAGGACCAAAGGACACTGACTGCTTTGCCTGCATGAACTTCAATGACAGTGGAGC
  CTGCGTTACTCAATGTCCCCAAACATTTGTCTACAATCCAACCACCTTTCAACTGGAACACAACTTCAAT
  GCAAAGTACACGTATGGAGCATTCTGTGTTAAGAAATGTCCACATAACTTCGTGGTAGATTCCAGTTCTT
  GTGTACGAGCCTGCCCTAGTTCTAAGATGGAAGTAGAAGAAAATGGGATTAAAATGTGTAAGCCTTGCAC
  CGATATTTGCCCCAAAGCATGTGATGGAATCGGCACGGGATCACTGATGTCTGCTCAGACTGTGGATTCA
  AGTAACATTGACAAATTCATAAACTGCACAAAGATCAATGGCAATCTCATCTTTCTTGTCACTGGCATTC
  ATGGAGACCCTTACAATGCTATTGACGCCATAGATCCAGAGAAACTGAATGTCTTTCGGACTGTCAGAGA
  AATAACAGGTTTCCTGAACATACAGTCTTGGCCCCCAAATATGACAGATTTCAGTGTTTTCTCCAACCTC
  GTCACAATTGGAGGAAGAGTCCTCTACAGTGGTCTCTCATTGCTGATCCTCAAACAACAAGGTATCACTT
  CCCTACAGTTCCAGTCTCTGAAGGAAATCAGTGCGGGCAATATCTACATCACTGACAACAGCAACCTGTG
  TTATTACCATACCATTAACTGGACAACACTCTTCAGCACCATTAACCAGAGAATAGTGATCCGAGATAAC
  AGAAGAGCTGAGAATTGTACTGCTGAAGGCATGGTATGCAACCACCTGTGTTCAAATGATGGTTGTTGGG
  GACCTGGGCCGGACCAGTGCCTGTCATGTCGGCGCTTCAGCAGGGGAAAGATCTGCATAGAGTCTTGCAA
  CCTTTATGATGGGGAATTTCGAGAGTTTGAAAACGGCTCCATCTGTGTTGAGTGTGACTCCCAGTGTGAG
  AAAATGGAAGATGGACTCCTCACATGCCATGGACCGGGACCTGACAACTGCACAAAGTGCTCTCATTTTA
  AGGATGGTCCAAACTGTGTGGAGAAATGTCCAGATGGCCTACAGGGAGCAAACAGTTTCATTTTTAAGTA
  TGCAGATCAGGATCGGGAGTGCCACCCTTGCCATCCAAACTGCACCCAGGGGTGTAACGGTCCCACTAGT
  CATGACTGCATTTACTACCCATGGACGGGCCATTCCACTTTACCACAACACGCTAGAACTCCACTGATTG
  CAGCCGGAGTCATTGGAGGCCTCTTCATCCTGGTGATCATGGCTTTGACATTTGCTGTCTATGTCAGAAG
  AAAGAGCATCAAAAAGAAACGTGCTTTGAGGAGATTCCTGGAGACAGAGCTGGTAGAGCCCTTAACTCCC
  AGTGGCACGGCACCCAATCAAGCTCAACTTCGCATTTTGAAGGAAACCGAACTAAAGAGGGTAAAGGTCC
  TTGGCTCGGGAGCTTTTGGAACCGTTTATAAAGGTATTTGGGTGCCTGAAGGTGAAACAGTGAAAATCCC
  TGTGGCTATAAAGATCCTCAATGAAACAACTGGCCCCAAAGCCAACGTGGAGTTCATGGATGAGGCTCTG
  ATCATGGCAAGTATGGATCACCCACACCTAGTTCGCCTATTGGGAGTGTGTCTGAGTCCCACTATCCAGT
  TGGTTACGCAGCTGATGCCGCATGGCTGCCTACTGGACTATGTTCATGAACACAAGGATAACATTGGATC
  ACAGCTGCTGTTGAACTGGTGTGTCCAGATTGCTAAGGGAATGATGTACCTAGAAGAAAGACGGCTTGTT
  CATCGGGATCTGGCAGCCCGCAATGTCTTAGTGAAATCTCCAAATCATGTTAAAATCACAGATTTTGGAC
  TGGCCCGGCTCTTGGAAGGAGATGAAAAAGAATACAATGCTGATGGTGGCAAGATGCCAATTAAATGGAT
  GGCTCTGGAATGTATACATTATAGGAAATTCACACATCAAAGTGATGTTTGGAGCTATGGCGTCACTATA
  TGGGAACTGATGACCTTTGGAGGAAAGCCCTATGATGGAATTCCAACCCGAGAAATCCCCGATTTACTGG
  AGAAAGGAGAGCGTCTGCCTCAGCCTCCCATCTGCACTATTGATGTTTACATGGTCATGGTCAAATGTTG
  GATGATCGATGCTGACAGCAGACCTAAATTCAAAGAACTGGCTGCTGAGTTTTCAAGAATGGCTAGAGAC
  CCTCAAAGATACCTAGTTATTCAGGGTGATGATCGTATGAAGCTTCCCAGTCCAAATGACAGCAAATTCT
  TCCAGAATCTCTTGGATGAAGAGGATTTGGAAGACATGATGGATGCTGAGGAATATTTGGTCCCCCAGGC
  TTTCAACATACCTCCTCCCATCTACACATCCAGAACAAGAATTGACTCCAATAGGAATCAGTTTGTGTAC
  CAAGATGGGGGCTTTGCTACACAACAAGGAATGCCCATGCCCTACAGAGCCACAACCAGCACCATACCAG
  AGGCTCCAGTAGCTCAGGGTGCAACGGCTGAGATGTTTGATGACTCCTGCTGTAATGGTACCCTACGAAA
  GCCAGTGGCACCCCATGTCCAAGAGGACAGTAGCACTCAGAGGTATAGTGCTGATCCCACAGTGTTCGCC
  CCAGAACGGAATCCTCGAGGAGAACTGGATGAAGAAGGCTACATGACTCCAATGCATGACAAGCCCAAAC
  AAGAATATCTGAATCCTGTGGAAGAGAACCCTTTTGTGTCCCGAAGGAAGAATGGAGATCTTCAAGCTTT
  AGATAATCCGGAGTATCACAGTGCTTCCAGCGGTCCACCCAAGGCGGAGGATGAATACGTGAATGAGCCT
  CTATACCTCAACACCTTCGCCAATGCCTTGGGGAGTGCAGAGTACATGAAAAACAGTGTACTGTCTGTGC
  CAGAGAAAGCCAAGAAAGCATTTGACAACCCCGACTACTGGAACCACAGCCTGCCACCCCGGAGCACCCT
  TCAGCACCCAGACTACCTGCAGGAATACAGCACAAAATATTTTTATAAACAGAATGGACGGATCCGCCCC
  ATTGTGGCAGAGAATCCTGAGTACCTCTCGGAGTTCTCGCTGAAGCCTGGCACTATGCTGCCCCCTCCGC
  CCTACAGACACCGGAATACTGTGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225933 representing NM_010154
  Red=Cloning site Green=Tags(s)
  
  MKLATGLWVWGSLLMAAGTVQPSASQSVCAGTENKLSSLSDLEQQYRALRKYYENCEVVMGNLEITSIEH
  NRDLSFLRSIREVTGYVLVALNQFRYLPLENLRIIRGTKLYEDRYALAIFLNYRKDGNFGLQELGLKNLT
  EILNGGVYVDQNKFLCYADTIHWQDIVRNPWPSNMTLVSTNGSSGCGRCHKSCTGRCWGPTENHCQTLTR
  TVCAEQCDGRCYGPYVSDCCHRECAGGCSGPKDTDCFACMNFNDSGACVTQCPQTFVYNPTTFQLEHNFN
  AKYTYGAFCVKKCPHNFVVDSSSCVRACPSSKMEVEENGIKMCKPCTDICPKACDGIGTGSLMSAQTVDS
  SNIDKFINCTKINGNLIFLVTGIHGDPYNAIDAIDPEKLNVFRTVREITGFLNIQSWPPNMTDFSVFSNL
  VTIGGRVLYSGLSLLILKQQGITSLQFQSLKEISAGNIYITDNSNLCYYHTINWTTLFSTINQRIVIRDN
  RRAENCTAEGMVCNHLCSNDGCWGPGPDQCLSCRRFSRGKICIESCNLYDGEFREFENGSICVECDSQCE
  KMEDGLLTCHGPGPDNCTKCSHFKDGPNCVEKCPDGLQGANSFIFKYADQDRECHPCHPNCTQGCNGPTS
  HDCIYYPWTGHSTLPQHARTPLIAAGVIGGLFILVIMALTFAVYVRRKSIKKKRALRRFLETELVEPLTP
  SGTAPNQAQLRILKETELKRVKVLGSGAFGTVYKGIWVPEGETVKIPVAIKILNETTGPKANVEFMDEAL
  IMASMDHPHLVRLLGVCLSPTIQLVTQLMPHGCLLDYVHEHKDNIGSQLLLNWCVQIAKGMMYLEERRLV
  HRDLAARNVLVKSPNHVKITDFGLARLLEGDEKEYNADGGKMPIKWMALECIHYRKFTHQSDVWSYGVTI
  WELMTFGGKPYDGIPTREIPDLLEKGERLPQPPICTIDVYMVMVKCWMIDADSRPKFKELAAEFSRMARD
  PQRYLVIQGDDRMKLPSPNDSKFFQNLLDEEDLEDMMDAEEYLVPQAFNIPPPIYTSRTRIDSNRNQFVY
  QDGGFATQQGMPMPYRATTSTIPEAPVAQGATAEMFDDSCCNGTLRKPVAPHVQEDSSTQRYSADPTVFA
  PERNPRGELDEEGYMTPMHDKPKQEYLNPVEENPFVSRRKNGDLQALDNPEYHSASSGPPKAEDEYVNEP
  LYLNTFANALGSAEYMKNSVLSVPEKAKKAFDNPDYWNHSLPPRSTLQHPDYLQEYSTKYFYKQNGRIRP
  IVAENPEYLSEFSLKPGTMLPPPPYRHRNTVV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010154
• ORF Size: 3876 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010154.2, NP_034284.1
• RefSeq Size: 4257 bp
• RefSeq ORF: 3879 bp
• Locus ID: 13869
• UniProt ID: Q61527
• Cytogenetics: 1 33.8 cM
• MW: 145.7 kDa
• Gene Summary: Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Required for mammary gland differentiation, induction of milk proteins and lactation. Acts as cell-surface receptor for the neuregulins NRG1, NRG2, NRG3 and NRG4 and the EGF family members BTC, EREG and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Ligand specificity and signaling is modulated by alternative splicing, proteolytic processing, and by the formation of heterodimers with other ERBB family members, thereby creating multiple combinations of intracellular phosphotyrosines that trigger ligand- and context-specific cellular responses. Mediates phosphorylation of SHC1 and activation of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Isoform JM-A CYT-1 and isoform JM-B CYT-1 phosphorylate PIK3R1, leading to the activation of phosphatidylinositol 3-kinase and AKT1 and protect cells against apoptosis. Isoform JM-A CYT-1 and isoform JM-B CYT-1 mediate reorganization of the actin cytoskeleton and promote cell migration in response to NRG1. Isoform JM-A CYT-2 and isoform JM-B CYT-2 lack the phosphotyrosine that mediates interaction with PIK3R1, and hence do not phosphorylate PIK3R1, do not protect cells against apoptosis, and do not promote reorganization of the actin cytoskeleton and cell migration. Proteolytic processing of isoform JM-A CYT-1 and isoform JM-A CYT-2 gives rise to the corresponding soluble intracellular domains (4ICD) that translocate to the nucleus, promote nuclear import of STAT5A, activation of STAT5A, mammary epithelium differentiation, cell proliferation and activation of gene expression. The ERBB4 soluble intracellular domains (4ICD) colocalize with STAT5A at the CSN2 promoter to regulate transcription of milk proteins during lactation. The ERBB4 soluble intracellular domains can also translocate to mitochondria and promote apoptosis.[UniProtKB/Swiss-Prot Function]