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Abca4 (NM_007378) Mouse Tagged ORF Clone
CAT#: MR212120
- TrueORF®
Abca4 (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family A (ABC1), member 4 (Abca4)
ORF Plasmid: tGFP
"NM_007378" in other vectors (2)
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | Abca4 |
| Synonyms | Abc1; Abc10; Abcr; AW050280; D430003I15Rik; R; RmP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR212120 representing NM_007378
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTTCCTCAGACAGATACAGCTTTTGCTTTGGAAGAACTGGACTCTGAGGAAAAGGCAGAAGATTC GCTTTGTAGTGGAACTCGTGTGGCCTTTGTCTTTGTTTTTGGTGTTAATCTGGCTGAGGAATGCCAACCC ACTCTATAGTCAGCATGAATGCCATTTTCCCAACAAGGCGATGCCTTCAGCAGGACTGTTACCGTGGCTC CAGGGGATTTTCTGCAATATGAACAACCCTTGTTTTCAAAACCCCACCCCTGGAGAGTCTCCTGGAACTG TCTCAAACTATAACAACTCCATCTTGGCAAGAGTATATCGAGATTTTCAAGAACTCTTCATGGACACCCC GGAGGTCCAGCACCTTGGCCAGGTTTGGGCCGAGCTCCGCACCTTGTCGCAGTTCATGGACACCCTGAGG ACTCACCCTGAGAGATTTGCAGGAAGAGGATTACAAATCCGAGACATCCTAAAAGATGAAGAGGCGCTGA CCCTATTTCTCATGAGAAACATTGGCCTGTCTGACTCGGTTGCCCATCTTCTGGTCAACTCCCAAGTTCG TGTGGAGCAGTTTGCTTATGGAGTCCCAGACTTGGAACTGACAGACATTGCCTGCAGCGAGGCCCTCCTG CAGCGCTTCATCATCTTCAGCCAGCGTCGGGGGGCACAGACGGTACGCGATGCCCTGTGTCCCCTCTCCC AGGTCACCCTACAGTGGATAGAAGACACTCTGTATGCCGATGTGGACTTCTTCAAACTCTTCCATGTGCT CCCCACACTCCTGGACAGCAGTTCTCAAGGAATCAACTTGAGATTTTGGGGAGGAATATTATCTGATCTG TCACCAAGAATGCAAAAGTTTATCCATCGGCCAAGTGTTCAAGACTTGCTATGGGTGAGCAGACCTCTCC TGCAGAACGGTGGTCCTGAGACCTTCACACAGCTGATGAGCATCCTGTCTGACCTCCTGTGTGGGTACCC AGAGGGAGGAGGCTCCCGAGTGTTCTCCTTTAACTGGTATGAAGACAATAACTATAAAGCCTTCCTGGGG ATTGATTCCACAAGGAAAGACCCCGCCTATTCTTACGACAAAAGAACAACATCCTTTTGTAATTCATTGA TCCAGAGCCTGGAGTCAAACCCTTTAACCAAAATAGCCTGGAGGGCGGCAAAGCCATTGCTGATGGGAAA AATCCTCTTTACTCCAGATTCCCCTGCTGCTCGAAGGATAATGAAGAATGCCAACTCAACTTTTGAAGAA CTGGATCGAGTTAGGAAGTTGGTAAAAGCCTGGGAGGAAGTGGGGCCCCAGATCTGGTACTTCTTTGAGA AGAGCACACAGATGACCGTGATCCGAGACACCCTGCAGCACCCAACCGTCAAAGACTTCATAAATAGGCA GCTCGGAGAAGAAGGCATTACCACCGAAGCCGTATTAAACTTCTTCTCTAACGGTCCCCAAGAGAAGCAG GCTGATGATATGACCAGCTTTGACTGGAGGGACATATTCAACATCACTGACCGATTCCTACGCTTGGCTA ATCAATACCTGGAGTGTCTGGTCCTGGATAAGTTTGAAAGTTATGATGATGAAGTGCAGCTCACCCAACG AGCCCTGTCTCTCCTGGAGGAGAACAGGTTCTGGGCCGGAGTGGTGTTCCCTGGCATGTATCCCTGGGCC AGCTCCTTACCTCCTCATGTGAAGTACAAGATTCGGATGGACATAGATGTGGTGGAGAAGACCAATAAGA TCAAAGACAGGTACTGGGATTCTGGTCCCAGGGCGGATCCTGTCGAAGATTTCCGGTACATCTGGGGAGG TTTTGCCTATCTACAGGACATGGTGGAGCAAGGAATCGTGAAGAGTCAGATGCAGGCAGAGCCTCCAATT GGAGTCTATCTCCAACAGATGCCTTATCCCTGCTTTGTGGATGACTCCTTCATGATCATCCTGAATCGCT GTTTCCCTATCTTCATGGTGCTGGCGTGGATCTACTCTGTCTCCATGACCGTTAAGGGCATTGTTTTGGA GAAGGAGCTGAGGCTGAAGGAGACCTTGAAAAACCAAGGCGTCTCTAATGCTGTCATCTGGTGTACCTGG TTCCTGGATAGCTTCTCCATCATGGCGCTGAGTATCTTCCTCCTGACGCTGTTCATCATGCATGGAAGGA TCCTCCATTACAGCGATCCCTTCATTCTCTTCCTGTTCTTGTTGGCCTTTGCCACTGCGACCATCATGCA GAGCTTCCTGCTCAGTACTTTGTTTTCCAAGGCCAGCCTTGCAGCAGCCTGCAGTGGGGTCATCTACTTC ACCCTCTACCTACCACACGTTCTGTGCTTTGCCTGGCAGGACCGGATGACGGCCGACCTGAAGACGACTG TGAGCCTACTTTCTTCCGTGGCATTTGGGTTTGGCACCGAGTACCTGGTCCGCTTTGAGGAGCAAGGCCT GGGGCTGCAATGGAGCAACATTGGGAAGAGTCCCCTGGAAGGGGATGAGTTCAGTTTCCTGCTGTCTATG AAGATGATGCTTCTCGATGCTGCTCTCTACGGCTTGCTTGCTTGGTATCTTGACCAGGTTTTCCCAGGAG ACTATGGGACCCCACTTCCCTGGTACTTCCTTCTGCAGGAGTCCTACTGGCTTGGTGGTGAAGGTTGTTC GACCAGAGAAGAAAGGGCTCTGGAAAAGACTGAACCCTTAACAGAGGAGATGGAGGATCCAGAGCACCCA GAAGGAATGAATGACTCCTTTTTTGAACGCGAGCTTCCAGGGCTGGTGCCTGGTGTGTGTGTGAAGAACC TGGTGAAGGTTTTTGAGCCCAGTGGCCGGCCAGCTGTGGACCGTCTGAACATCACTTTCTATGAGAACCA AATCACGGCGTTCCTGGGTCACAACGGAGCGGGAAAGACCACCACCTTGTCCATCCTGACAGGACTGTTG CCACCGACGTCAGGAACTGTGCTCATTGGGGGAAAAGACATTGAAACCAACCTGGATGTAGTACGGCAGA GCCTGGGCATGTGTCCACAACACAACATCCTGTTTCATCACCTCACGGTGGCTGAGCACATCTTGTTCTA TGCCCAGCTGAAAGGGAGATCCTGGGAGGAGGCCCAGCTTGAGATGGAAGCCATGCTAGAAGACACGGGC CTCCACCATAAGAGGAATGAAGAAGCTCAGGACCTTTCAGGTGGCATGCAGAGGAAGCTGTCTGTTGCCA TTGCTTTTGTGGGAGATTCTAAGGTGGTGGTCCTGGATGAGCCCACCTCTGGGGTGGACCCCTACTCCAG ACGCTCCATCTGGGACCTGCTCCTGAAGTATCGCTCAGGCAGAACCATCATCATGTCCACTCACCACATG GACGAGGCAGACCTCCTTGGGGACCGCATTGCCATCATTTCTCAGGGAAGGCTCTACTGCTCTGGAACCC CGCTCTTCCTCAAGAACTGCTTTGGCACAGGCTTCTACTTGACCTTAGTTCGCAAGATGAAAAACATCCA GAGCCAAAGAGGTGGCTGTGAGGGGGTCTGCAGCTGTACATCAAAGGGTTTCTCTACCAGGTGTCCAACC CGAGTCGATGAGATAACAGAAGAACAAGTCCTGGATGGAGATGTGCAGGAGCTGATGGATTTGGTATACC ACCATGTCCCAGAGGCAAAGCTGGTGGAATGCATTGGTCAAGAACTTATCTTCCTCCTTCCAAACAAGAA TTTCAAGCAGAGAGCATATGCCAGCCTTTTCCGAGAGCTGGAGGAGACTCTGGCTGACCTGGGGCTCAGC AGCTTTGGAATTTCTGACACTCCCCTGGAAGAGATTTTCCTGAAGGTCACAGAGGATGCTGGAGCGGGCT CTATGTTTGTAGGTGGCGCTCAGCAGAAAAGAGAACAAGCCGGTCTCCGGCACCCTTGTTCGGCTCCCAC TGAGAAGCTCAGGCAGTATGCCCAGGCCCCACATACCTGTTCCCCAGGACAAGTGGATCCTCCCAAGGGC CAACCTTCCCCAGAGCCAGAGGACCCCGGTGTCCCATTCAACACAGGTGCTCGGCTGATTCTTCAACATG TGCAGGCCCTGCTAGTCAAGCGATTCCATCACACCATCCGCAGCCGCAAGGACTTTGTGGCTCAGATTGT TCTCCCTGCCACTTTCGTGTTCTTGGCTCTGATGCTTTCAATCATTGTGCCTCCGTTTGGTGAATTTCCA GCTTTGACTCTTCATCCCTGGATGTATGGGCATCAATATACCTTCTTCAGCATGGACGAACCCAACAATG AACACCTTGAAGTACTGGCAGATGTCCTTCTGAACAGGCCAGGCTTTGGCAACCGTTGTCTAAAGGAAGA GTGGCTTCCGGAGTACCCATGCATTAATGCAACCTCCTGGAAGACGCCCTCCGTGTCCCCGAACATCACC CACCTGTTCCAGAAGCAGAAATGGACAGCAGCCCACCCCTCTCCCTCCTGCAAGTGCAGCACCAGAGAGA AGCTCACCATGTTGCCCGAGTGCCCCGAGGGTGCTGGAGGGCTCCCACCCCCACAGAGGACACAGCGCAG CACTGAAGTCCTACAAGACCTCACGAACAGGAACATCTCCGACTACTTGGTAAAAACGTACCCTGCTCTC ATAAGAAGCAGCTTAAAGAGCAAATTCTGGGTCAATGAACAGAGGTATGGAGGAATTTCCATCGGAGGAA AACTCCCTGCTATCCCCATCAGCGGGGAAGCACTTGTTGGCTTTCTAAGTGGCCTTGGCCAGATGATGAA CGTGAGCGGGGGTCCTGTCACCAGGGAGGCCTCCAAAGAAATGTTAGATTTCCTCAAACATCTTGAAACC ACAGACAACATTAAGGTATGGTTTAACAACAAGGGCTGGCATGCCCTGGTCAGCTTTCTGAATGTGGCTC ACAACGCCATCTTACGGGCCAGCCTGCCCAGGGACAGGGACCCTGAGGAGTATGGAATCACTGTCATCAG CCAGCCTCTGAACCTGACCAAGGAGCAGCTCTCGGACATCACAGTACTGACTACTTCCGTGGATGCCGTG GTTGCCATATGTGTGATTTTCGCCATGTCCTTCGTTCCAGCCAGCTTTGTCCTTTACTTGATCCAGGAGA GGGTGACGAAAGCTAAACATTTGCAGTTTATCAGTGGTGTGAGCCCCACCACCTACTGGCTGACCAACTT CCTCTGGGATATTATGAATTATGCTGTGAGTGCGGGATTGGTGGTGGGCATCTTCATTGGATTTCAAAAG AAAGCCTACACATCCCCAGACAACCTTCCTGCTCTCGTCTCTCTGCTCATGCTGTATGGATGGGCAGTCA TTCCTATGATGTATCCAGCATCCTTCCTGTTTGAAGTCCCCAGCACAGCCTATGTGGCTTTGTCCTGTGC TAACCTGTTCATCGGCATCAACAGCAGCGCCATCACCTTCGTCCTGGAATTATTTGAGAATAACCGGACG CTGCTCAGGTTCAATGCCATGTTGAGGAAGTTGCTCATTGTCTTCCCCCACTTCTGCCTGGGCAGGGGCC TCATTGACCTGGCACTGAGCCAAGCTGTGACAGACGTCTATGCCCAGTTTGGTGAGGAGTACTCTGCAAA CCCATTCCAGTGGGACCTGATTGGGAAGAACCTGGTTGCTATGGCAATAGAAGGGGTGGTATACTTCCTT CTGACCCTGCTCATCCAACACCATTTTTTCCTCACCCGGTGGATCGCTGAGCCTGCTAGAGAGCCCGTTT TTGATGAAGATGACGATGTGGCTGAAGAAAGACAAAGAGTTATGAGTGGGGGAAATAAAACCGACATCTT AAAGCTAAACGAACTAACCAAGGTTTACTCAGGCTCTTCCAGTCCAGCAGTAGATCGGTTATGTGTTGGA GTTCGACCTGGAGAGTGCTTTGGCCTCCTGGGAGTGAATGGTGCAGGCAAAACCACCACATTCAAGATGC TCACTGGGGACACCACAGTGACATCGGGGGATGCTACTGTGGCAGGCAAGAGCATTTTAACTAGTATTTC TGATGTCCATCAAAACATGGGCTACTGTCCTCAGTTTGATGCAATCGATGACCTGCTCACAGGCAGAGAA CACCTTTACCTCTATGCCAGGCTGCGGGGCGTGCCATCGAAGGAAATCGAGAAGGTTGCCAACTGGGGTA TCCAGAGCCTGGGCTTGTCTCTCTACGCTGACCGCCTGGCAGGCACCTACAGTGGAGGCAATAAGAGGAA ACTCTCTACAGCCATAGCTCTCACTGGCTGCCCTCCCCTGCTGCTGCTGGATGAGCCCACGACAGGGATG GATCCCCAGGCACGCCGCATGCTGTGGAACACCATTGTGAGCATCATCAGAGAAGGGAGAGCTGTGGTTC TCACCTCCCACAGCATGGAAGAATGTGAAGCTCTGTGTACGCGGCTGGCCATCATGGTGAAGGGCACCTT TCAGTGTCTGGGTACCATCCAACACCTCAAGTACAAGTTTGGAGACGGCTACATTGTCACAATGAAAATC AAATCTCCAAAGGACGACTTGCTTCCCGATTTGAATCCTGTGGAGCAGTTCTTCCAGGGCAACTTCCCTG GCAGCGTGCAGAGGGAGAGACACCACAGCATGCTCCAGTTCCAGGTCCCCTCGTCCTCCCTGGCCAGGAT CTTCCAGCTGCTCATTTCCCACAAGGACAGCCTGCTCATCGAGGAGTACTCAGTCACCCAGACCACGCTG GACCAGGTGTTTGTAAACTTCGCTAAACAGCAGACTGAGACCTATGACCTCCCTCTGCACCCCCGGGCTG CTGGAGCCAGCTGGCAAGCCAAGCTTGAAGAGAAATCCGGACGGCTGCAGACACAGGAGCCTTTGCCCGC AGGATCTGAACAACTGGCTAACGGAAGCAACCCCACGGCAGCAGAAGATAAGCACACCAGGAGTCCACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR212120 representing NM_007378
Red=Cloning site Green=Tags(s) MGFLRQIQLLLWKNWTLRKRQKIRFVVELVWPLSLFLVLIWLRNANPLYSQHECHFPNKAMPSAGLLPWL QGIFCNMNNPCFQNPTPGESPGTVSNYNNSILARVYRDFQELFMDTPEVQHLGQVWAELRTLSQFMDTLR THPERFAGRGLQIRDILKDEEALTLFLMRNIGLSDSVAHLLVNSQVRVEQFAYGVPDLELTDIACSEALL QRFIIFSQRRGAQTVRDALCPLSQVTLQWIEDTLYADVDFFKLFHVLPTLLDSSSQGINLRFWGGILSDL SPRMQKFIHRPSVQDLLWVSRPLLQNGGPETFTQLMSILSDLLCGYPEGGGSRVFSFNWYEDNNYKAFLG IDSTRKDPAYSYDKRTTSFCNSLIQSLESNPLTKIAWRAAKPLLMGKILFTPDSPAARRIMKNANSTFEE LDRVRKLVKAWEEVGPQIWYFFEKSTQMTVIRDTLQHPTVKDFINRQLGEEGITTEAVLNFFSNGPQEKQ ADDMTSFDWRDIFNITDRFLRLANQYLECLVLDKFESYDDEVQLTQRALSLLEENRFWAGVVFPGMYPWA SSLPPHVKYKIRMDIDVVEKTNKIKDRYWDSGPRADPVEDFRYIWGGFAYLQDMVEQGIVKSQMQAEPPI GVYLQQMPYPCFVDDSFMIILNRCFPIFMVLAWIYSVSMTVKGIVLEKELRLKETLKNQGVSNAVIWCTW FLDSFSIMALSIFLLTLFIMHGRILHYSDPFILFLFLLAFATATIMQSFLLSTLFSKASLAAACSGVIYF TLYLPHVLCFAWQDRMTADLKTTVSLLSSVAFGFGTEYLVRFEEQGLGLQWSNIGKSPLEGDEFSFLLSM KMMLLDAALYGLLAWYLDQVFPGDYGTPLPWYFLLQESYWLGGEGCSTREERALEKTEPLTEEMEDPEHP EGMNDSFFERELPGLVPGVCVKNLVKVFEPSGRPAVDRLNITFYENQITAFLGHNGAGKTTTLSILTGLL PPTSGTVLIGGKDIETNLDVVRQSLGMCPQHNILFHHLTVAEHILFYAQLKGRSWEEAQLEMEAMLEDTG LHHKRNEEAQDLSGGMQRKLSVAIAFVGDSKVVVLDEPTSGVDPYSRRSIWDLLLKYRSGRTIIMSTHHM DEADLLGDRIAIISQGRLYCSGTPLFLKNCFGTGFYLTLVRKMKNIQSQRGGCEGVCSCTSKGFSTRCPT RVDEITEEQVLDGDVQELMDLVYHHVPEAKLVECIGQELIFLLPNKNFKQRAYASLFRELEETLADLGLS SFGISDTPLEEIFLKVTEDAGAGSMFVGGAQQKREQAGLRHPCSAPTEKLRQYAQAPHTCSPGQVDPPKG QPSPEPEDPGVPFNTGARLILQHVQALLVKRFHHTIRSRKDFVAQIVLPATFVFLALMLSIIVPPFGEFP ALTLHPWMYGHQYTFFSMDEPNNEHLEVLADVLLNRPGFGNRCLKEEWLPEYPCINATSWKTPSVSPNIT HLFQKQKWTAAHPSPSCKCSTREKLTMLPECPEGAGGLPPPQRTQRSTEVLQDLTNRNISDYLVKTYPAL IRSSLKSKFWVNEQRYGGISIGGKLPAIPISGEALVGFLSGLGQMMNVSGGPVTREASKEMLDFLKHLET TDNIKVWFNNKGWHALVSFLNVAHNAILRASLPRDRDPEEYGITVISQPLNLTKEQLSDITVLTTSVDAV VAICVIFAMSFVPASFVLYLIQERVTKAKHLQFISGVSPTTYWLTNFLWDIMNYAVSAGLVVGIFIGFQK KAYTSPDNLPALVSLLMLYGWAVIPMMYPASFLFEVPSTAYVALSCANLFIGINSSAITFVLELFENNRT LLRFNAMLRKLLIVFPHFCLGRGLIDLALSQAVTDVYAQFGEEYSANPFQWDLIGKNLVAMAIEGVVYFL LTLLIQHHFFLTRWIAEPAREPVFDEDDDVAEERQRVMSGGNKTDILKLNELTKVYSGSSSPAVDRLCVG VRPGECFGLLGVNGAGKTTTFKMLTGDTTVTSGDATVAGKSILTSISDVHQNMGYCPQFDAIDDLLTGRE HLYLYARLRGVPSKEIEKVANWGIQSLGLSLYADRLAGTYSGGNKRKLSTAIALTGCPPLLLLDEPTTGM DPQARRMLWNTIVSIIREGRAVVLTSHSMEECEALCTRLAIMVKGTFQCLGTIQHLKYKFGDGYIVTMKI KSPKDDLLPDLNPVEQFFQGNFPGSVQRERHHSMLQFQVPSSSLARIFQLLISHKDSLLIEEYSVTQTTL DQVFVNFAKQQTETYDLPLHPRAAGASWQAKLEEKSGRLQTQEPLPAGSEQLANGSNPTAAEDKHTRSPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007378 |
| ORF Size | 6930 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007378.1, NP_031404.1 |
| RefSeq Size | 7268 bp |
| RefSeq ORF | 6933 bp |
| Locus ID | 11304 |
| UniProt ID | O35600 |
| Cytogenetics | 3 52.94 cM |
| MW | 260.7 kDa |
| Gene Summary | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This protein was the first of the ABC transporters to be observed in photoreceptors and may play a role in the photoresponse. Mutations in the human gene are found in patients diagnosed with Stargardt disease and are associated with retinitis pigmentosa-19 and macular degeneration age-related 2. [provided by RefSeq, Jul 2008] |
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