Aatf (NM_019816) Mouse Tagged ORF Clone

CAT#: MR208444

Aatf (Myc-DDK-tagged) - Mouse apoptosis antagonizing transcription factor (Aatf)

Product Images

Circular map for MR208444

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aatf
• Synonyms: 4933415H02Rik; 5830465M17Rik; Che-1; Trb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208444 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCCGCAGCCCTTGGCTCTGCAGCTGGAACAGTTGCTGAATCCGAGACCGCGTGAGGCGGATC
  CTGAGGCCGACCCGGAGGAGGCCACTAGAGCCAGAGTGATTGACAGGTTTGATGAAGGGGAAGAGGAGAA
  AGATGATCTGGCAGTGAGTAGCATTAGAAAGTTGGCACCAGTCTCCCTCTTGGACACCGACAAAAGGTAT
  TCTGGCAAGACCACTTCTAGGAAAGCTTGGAAAGAAGACCATTGGGAGCAGGCTCTGCCAAGTTCATCTG
  ATAACGAAGCATCTGATGAGGGAGGATCTGAAGATGGGGATTCAGAGGGCCTGGGCCTGGAGGAGATCTC
  AGAAGATGTTGACGAAGACTTAGAAGACAACAAAATATCTGATGAGGGAGGATCTGAAGATGGGGATTCA
  GAGGGCCTGGGCCTGGAAGAGTTCTCAGAAGATGTTGAGGAGGACTTAGAAGGTGAGGATGAGGAAGACA
  GAGAAGAAGACAGGAACAGTGAAGATGATGGTGTGGTGGCAGCCTTCTCCAGTGTCAAAGTTTCTGAGGA
  AGTGGAAAAAGGAAGAGCTGTGAAGAATCAGATAGCATTGTGGGACCAGCTCTTGGAAGGAAGGATCAAG
  CTACAAAAAGCTCTCTTGACTACCAATCAGTTGCCTCAACCAGATGTTTTCCCTGTCTTCAAGGACAAAG
  GTGGCCCAGAATTTGCCAGTGCTCTGAAAAATAGTCACAAAGCACTTAAAGCCTTGTTAAGGTCATTGGT
  GGATCTTCAGGAAGAGTTGCTTTTCCAGTACCCAGACACGAGACATATAGTAAATGGGGCGAAACCCAAT
  ACAGAGAGTGAGGAGATTTCCAGTGAAGATGATGAGCTAGTGGGAGAGAAGAAGAAACAACGAAAGGCCC
  CACCCAAGAGGAAGCTAGAGATGGAGGACTATCCCAGCTTCATGGCAAAGCGCTTTGCTGACTTCACCAT
  CTATAGGAATCACACACTACAGAAATGGCATGATAAAACCAAGCTTGCTTCTGGAAAACTGGGCAAGGGT
  TTTGGTGCCTTCGAACGCTCGATCTTGACTCAGATCGATCATATCATGATGGACAAAGAAAGATTGCTTC
  GGAGGACACAGACCAAGCGCTCTGCCTACCGAGTTCTTGGCAAACCGGAGCCAGTCCCTGAGCCTGTTGC
  AGAGACCTTGCCAGGAGAACCAGAGACGCTTCCTCAGGGCCCTGCCAATGCCCACCTGAGGGACTTGGAT
  GAGGAGATATTTGATGATGATGACTTTTACCACCAGCTCCTTCGAGAACTCATAGAGCGGAAAACCAGCT
  CTTTGGATCCAAATGATCAGGTGGCCATGGGAAGGCAGTGGCTTGCAATCCAGAAACTACGAAGCAAAAT
  CCGCAAGAAGGTAGATAGAAAAGCCAGCAAAGGAAGGAAACTTCGGTTCCATGTCCTTAGCAAGCTCCTG
  AGTTTCATGGCACCTATTGACCACACCGCAATGAGTGATGATGCCAGGACGGAGTTGTTTCGATCTCTTT
  TCGGCCAGCTCAACCCTCCTGATGCAGACCGTGGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208444 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPQPLALQLEQLLNPRPREADPEADPEEATRARVIDRFDEGEEEKDDLAVSSIRKLAPVSLLDTDKRY
  SGKTTSRKAWKEDHWEQALPSSSDNEASDEGGSEDGDSEGLGLEEISEDVDEDLEDNKISDEGGSEDGDS
  EGLGLEEFSEDVEEDLEGEDEEDREEDRNSEDDGVVAAFSSVKVSEEVEKGRAVKNQIALWDQLLEGRIK
  LQKALLTTNQLPQPDVFPVFKDKGGPEFASALKNSHKALKALLRSLVDLQEELLFQYPDTRHIVNGAKPN
  TESEEISSEDDELVGEKKKQRKAPPKRKLEMEDYPSFMAKRFADFTIYRNHTLQKWHDKTKLASGKLGKG
  FGAFERSILTQIDHIMMDKERLLRRTQTKRSAYRVLGKPEPVPEPVAETLPGEPETLPQGPANAHLRDLD
  EEIFDDDDFYHQLLRELIERKTSSLDPNDQVAMGRQWLAIQKLRSKIRKKVDRKASKGRKLRFHVLSKLL
  SFMAPIDHTAMSDDARTELFRSLFGQLNPPDADRGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019816
• ORF Size: 1581 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019816.1, NP_062790.1
• RefSeq Size: 1844 bp
• RefSeq ORF: 1581 bp
• Locus ID: 56321
• UniProt ID: Q9JKX4
• Cytogenetics: 11 C
• MW: 59.5 kDa
• Gene Summary: May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression (By similarity).[UniProtKB/Swiss-Prot Function]