Glis2 (NM_031184) Mouse Tagged ORF Clone

CAT#: MR208349

Glis2 (Myc-DDK-tagged) - Mouse GLIS family zinc finger 2 (Glis2)

Product Images

Circular map for MR208349

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Glis2
• Synonyms: Gli5; Klf16; Nkl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208349 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACTCCTTGGACGAGCCCCTCGACCTAAAGCTGAGCATCACCAAGCTCCGAGCGGCAAGAGAGAAGA
  GAGAAAGGACACTGGGTGTGGTCCGGCATCATGCTTTGCATCGAGAGCTGGGCCTGGTGGACGATAGCCC
  CGCCCCTGGCTCCCCAGGCTCTCCACCACCAGGTTTCCTGCTGAACCCCAAATTCCCTGAGAAGGTGGAC
  GGACGCTTTTCTGCAGCCCCCTTGGTGGACCTCAGCTTGTCACCACCCTCTGGACTGGACTCTCCCAATG
  GCAGCAGCTCCCTGTCTCCTGAGTGCCAGGGCAATGGGGACTTGCCTCCACTGCCTACTGCTGTGGATTT
  CCAGCCACTTCGCTATTTGGATGGTGTCCCCAGTTCCTTCCAGTTCTTCTTGCCCCTGGGTTCTGGTGGG
  GCTCTGCACCTACCTGCTTCCTCCTTCCTTCCCCCCCCCAAGGACAAGTGCCTCTCACCAGAGCTGCCCC
  TGGCCAAGCAGCTGGTGTGTCGATGGGCCAAGTGTAACCAGCTCTTTGAGCTCCTCCAAGACCTGGTTGA
  CCATGTCAACGACCATCATGTCAAGCCTGAACAGGATGCTCGATACTGCTGTCATTGGGAGGGCTGCGCC
  CGCCATGGCCGTGGCTTCAATGCCAGGTACAAGATGCTCATTCACATCCGGACACACACCAACGAGAAGC
  CGCACCGATGCCCCACCTGTAACAAGAGCTTCTCCCGCCTGGAGAACCTGAAGATCCACAACCGCTCCCA
  CACAGGTGAGAAGCCCTACGTCTGCCCCTATGAGGGCTGCAACAAGCGTTACTCCAACTCCAGTGACCGC
  TTCAAGCACACCCGTACCCACTACGTAGACAAGCCCTACTACTGCAAGATGCCCGGCTGTCACAAGCGTT
  ACACGGACCCCAGTTCACTGCGCAAACACATCAAGGCCCATGGCCACTTTGTGTCACATGAGCAGCAGGA
  GCTCCTGCAGCTGCGCCCACCCCCTAAACCACCACTGCCCACTCCTGACAGTGGCTCCTATGTCAGCGGG
  GCTCAGATCATCATCCCGAACCCTGCTGCCCTTTTTGGAGGCCCCAGTCTGCCTGGCCTGCCATTACCTC
  TACCTCCTGGCCCCCTTGACCTCAGTGCTCTGGCCTGTGGCAATGGTGGAGGTGGGGGTGGGGGTATTGG
  CCCTGGGCTGCCAGGCTCTGTTCTGCCCCTCAATCTGGCCAAGAACCCGCTGTTGCCCTCACCCTTTGGG
  GCTGGTGGACTAGGCCTGCCTGTGGTTTCTCTCCTGGGTGGCTCTGCCGGTAGCAAGGCCGAGGGGGAGA
  AGGGTCGTGGGTCAGTACCTGCCAGGGTCCTGGGCCTGGAAGATCACAAGACGCCCCTGGAAAGGACGGA
  GCGCAGCCGCTCCCGGCCAAGCCCTGATGGACTCCCTTTGCTCCCAGGCACTGTACTGGACCTGTCCACA
  GGCAACTCAGCAGCCAGCAGTCCAGAGGTGTTAACTCCTGGCTGGGTGGTCATTCCACCAGGGTCTGTGC
  TGCTCAAACCAGCTGTGGTAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208349 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHSLDEPLDLKLSITKLRAAREKRERTLGVVRHHALHRELGLVDDSPAPGSPGSPPPGFLLNPKFPEKVD
  GRFSAAPLVDLSLSPPSGLDSPNGSSSLSPECQGNGDLPPLPTAVDFQPLRYLDGVPSSFQFFLPLGSGG
  ALHLPASSFLPPPKDKCLSPELPLAKQLVCRWAKCNQLFELLQDLVDHVNDHHVKPEQDARYCCHWEGCA
  RHGRGFNARYKMLIHIRTHTNEKPHRCPTCNKSFSRLENLKIHNRSHTGEKPYVCPYEGCNKRYSNSSDR
  FKHTRTHYVDKPYYCKMPGCHKRYTDPSSLRKHIKAHGHFVSHEQQELLQLRPPPKPPLPTPDSGSYVSG
  AQIIIPNPAALFGGPSLPGLPLPLPPGPLDLSALACGNGGGGGGGIGPGLPGSVLPLNLAKNPLLPSPFG
  AGGLGLPVVSLLGGSAGSKAEGEKGRGSVPARVLGLEDHKTPLERTERSRSRPSPDGLPLLPGTVLDLST
  GNSAASSPEVLTPGWVVIPPGSVLLKPAVVN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031184
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031184.3, NP_112461.2
• RefSeq Size: 3619 bp
• RefSeq ORF: 1566 bp
• Locus ID: 83396
• UniProt ID: Q8VDL9
• Cytogenetics: 16 2.45 cM
• MW: 55.8 kDa
• Gene Summary: Can act either as a transcriptional repressor or as a transcriptional activator, depending on the cell context. Acts as a repressor of the Hedgehog signaling pathway. Represses the Hedgehog-dependent expression of Wnt4. Necessary to maintain the differentiated epithelial phenotype in renal cells through the inhibition of SNAI1, which itself induces the epithelial-to-mesenchymal transition. Represses transcriptional activation by CTNNB1 in the Wnt signaling pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation.[UniProtKB/Swiss-Prot Function]