BC100379 Mouse Tagged ORF Clone

CAT#: MR201192

  • TrueORF®

(Myc-DDK-tagged) - Mouse cDNA clone MGC:117858 IMAGE:30460254

ORF Plasmid: DDK tGFP


  "BC100379" in other vectors (3)


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Reconstitution Protocol

USD 165.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

USD 198.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR201192 representing BC100379
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCTATGGGTCGGCAGGACCGCAGGCTGCAGCAGCAGCTTAATGAAAGCAGGAGCCGCTTCCAGA
CGTTAATGAAGCGGCTGATAGCGAAGTACAACCAGCCCTTTAAGGATAACTATGATGAGCTTAAAAAAGA
ATTCAACAGACTTTATCAGAAGTATTGTCTGTCACCGCAGCGGGCGAAGGTGACTTCGTGTGGCAGAGTA
TCGCCAATGAAAGCTGCTGCAGCTCTTCCCTGTCAATCAGAGCACTTGAAGAGACTAAATCCAGACTCCC
CACAACAGAGTTCCCAGAAACGGTCAATCTCTCCTGGCTGTCACAGAAGAGTCCTGCAGGATTCAACTCC
ACAGACTGCTAGTACACTTGTGAGAGAGCCTTGGCTTCCAACAAAGAGGCGCAAGTTGTCATACCCGGTA
GCTTGTGCTCACCAGGCCAAATCTCACAATACCTCAGGAGCATCAGGCTGGCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR201192 representing BC100379
Red=Cloning site Green=Tags(s)

MESMGRQDRRLQQQLNESRSRFQTLMKRLIAKYNQPFKDNYDELKKEFNRLYQKYCLSPQRAKVTSCGRV
SPMKAAAALPCQSEHLKRLNPDSPQQSSQKRSISPGCHRRVLQDSTPQTASTLVREPWLPTKRRKLSYPV
ACAHQAKSHNTSGASGWP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC100379
ORF Size 474 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC100379.1
RefSeq Size 2008 bp
RefSeq ORF 476 bp
MW 73.6 kDa

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.