Abcg1 (NM_009593) Mouse Tagged ORF Clone

CAT#: MG227241

Abcg1 (tGFP-tagged) - Mouse ATP-binding cassette sub-family G (WHITE) member 1 (Abcg1), (10ug)

Product Images

Circular map for MG227241

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Abcg1
• Synonyms: Abc; Abc8; AW413978; Whit; White
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227241 representing NM_009593
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGTCTGATGGCCGCTTTCTCGGTCGGCACCGCCATGAATGCCAGCAGCTACTCTGCCGCAATGA
  CGGAGCCCAAGTCCGTGTGCGTGTCAGTGGACGAGGTCGTGTCCAGCAACGTGGATGAGGTTGAGACAGA
  CCTGCTCAATGGGCACCTGAAGAAGGTGGACAACAACTTCACAGAGGCCCAGCGCTTTTCCTCCCTTCCG
  CGGAGGGCGGCCGTGAACATCGAATTCAAGGACCTTTCCTACTCTGTACCCGAGGGGCCCTGGTGGAAGA
  AGAAAGGATACAAGACCCTTTTGAAAGGGATCTCTGGGAAATTCAACAGTGGAGAGCTGGTGGCCATCAT
  GGGTCCTTCTGGAGCTGGGAAGTCCACACTCATGAATATTCTGGCAGGATACAGGGAGACTGGCATGAAA
  GGGGCAGTCCTTATCAATGGAATGCCCCGGGACCTGCGCTGCTTCCGGAAGGTCTCCTGCTACATCATGC
  AGGACGACATGCTGCTGCCTCACCTCACTGTTCAGGAGGCCATGATGGTGTCCGCGCATCTGAAGCTGCA
  GGAGAAGGATGAAGGCAGACGGGAGATGGTCAAAGAGATCCTGACAGCCCTGGGCTTGCTGCCCTGTGCC
  AACACACGCACGGGGAGCCTCTCAGGCGGCCAGCGGAAACGCCTGGCCATTGCCCTGGAGCTGGTCAACA
  ACCCGCCTGTCATGTTCTTTGATGAGCCCACCAGTGGCCTGGACAGCGCCTCCTGCTTCCAAGTGGTGTC
  TCTGATGAAAGGACTGGCCCAGGGTGGCCGCTCCATCGTCTGTACCATCCACCAGCCCAGTGCCAAGCTC
  TTTGAGCTCTTTGACCAGCTTTATGTCCTAAGTCAAGGACAATGCGTATACAGGGGAAAGGTCTCCAATC
  TCGTGCCGTATCTGAGGGATCTGGGTCTGAACTGCCCTACCTACCACAACCCAGCAGACTTTGTCATGGA
  AGTGGCATCAGGGGAGTACGGGGATCAGAACAGTCGCCTGGTGAGAGCCGTGCGAGAGGGCATGTGTGAC
  GCTGACTATAAGAGAGACCTCGGGGGCGACACCGATGTGAACCCGTTTCTTTGGCACCGGCCTGCTGAAG
  AGGACTCCGCCTCCATGGAAGGTTGCCATAGCTTCTCGGCCAGCTGCCTCACCCAGTTCTGCATCCTCTT
  CAAGAGGACCTTCCTCAGCATCATGCGGGACTCGGTCCTGACACATCTGCGAATCACCTCGCACATTGGG
  ATCGGCCTGCTCATTGGCCTGCTGTACCTGGGGATTGGGAATGAAGCCAAGAAGGTCCTTAGCAACTCCG
  GCTTCCTGTTCTTCTCCATGCTGTTCCTCATGTTTGCTGCCCTCATGCCCACTGTTCTGACCTTTCCCCT
  GGAGATGAGTGTCTTCCTCCGGGAGCACCTGAACTACTGGTACAGCCTGAAGGCCTACTACCTGGCAAAG
  ACCATGGCCGATGTCCCCTTTCAGATCATGTTCCCTGTGGCCTACTGCAGTATCGTATACTGGATGACGT
  CCCAGCCGTCGGACGCTGTGCGTTTTGTGCTGTTCGCTGCTCTGGGTACCATGACATCGCTGGTGGCCCA
  GTCCTTAGGACTACTGATTGGAGCTGCATCCACATCCCTGCAGGTTGCGACATTTGTGGGTCCCGTGACA
  GCCATCCCCGTCCTGCTCTTCTCCGGATTCTTTGTCAGCTTTGACACCATCCCAGCCTACCTGCAGTGGA
  TGTCCTACATCTCCTATGTCAGATACGGCTTTGAGGGGGTCATCCTGTCCATCTACGGCTTGGACCGAGA
  AGACCTGCACTGCGACATCGCGGAGACATGCCACTTCCAGAAGTCAGAGGCCATCCTGAGGGAGCTGGAC
  GTGGAGAATGCGAAGCTGTACCTGGATTTCATCGTCCTGGGCATCTTCTTCATCTCCCTGCGGCTCATCG
  CCTATTTCGTCCTCAGATACAAAATCCGGGCTGAGAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227241 representing NM_009593
  Red=Cloning site Green=Tags(s)
  
  MACLMAAFSVGTAMNASSYSAAMTEPKSVCVSVDEVVSSNVDEVETDLLNGHLKKVDNNFTEAQRFSSLP
  RRAAVNIEFKDLSYSVPEGPWWKKKGYKTLLKGISGKFNSGELVAIMGPSGAGKSTLMNILAGYRETGMK
  GAVLINGMPRDLRCFRKVSCYIMQDDMLLPHLTVQEAMMVSAHLKLQEKDEGRREMVKEILTALGLLPCA
  NTRTGSLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKGLAQGGRSIVCTIHQPSAKL
  FELFDQLYVLSQGQCVYRGKVSNLVPYLRDLGLNCPTYHNPADFVMEVASGEYGDQNSRLVRAVREGMCD
  ADYKRDLGGDTDVNPFLWHRPAEEDSASMEGCHSFSASCLTQFCILFKRTFLSIMRDSVLTHLRITSHIG
  IGLLIGLLYLGIGNEAKKVLSNSGFLFFSMLFLMFAALMPTVLTFPLEMSVFLREHLNYWYSLKAYYLAK
  TMADVPFQIMFPVAYCSIVYWMTSQPSDAVRFVLFAALGTMTSLVAQSLGLLIGAASTSLQVATFVGPVT
  AIPVLLFSGFFVSFDTIPAYLQWMSYISYVRYGFEGVILSIYGLDREDLHCDIAETCHFQKSEAILRELD
  VENAKLYLDFIVLGIFFISLRLIAYFVLRYKIRAER
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009593
• ORF Size: 1998 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009593.1
• RefSeq Size: 5821 bp
• RefSeq ORF: 2001 bp
• Locus ID: 11307
• UniProt ID: Q64343
• Cytogenetics: 17 A3.3
• Gene Summary: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The human protein is involved in macrophage cholesterol efflux and may regulate cellular lipid homeostasis in other cell types. [provided by RefSeq, Jul 2008]