Abca5 (NM_147219) Mouse Tagged ORF Clone

CAT#: MG222272

Abca5 (tGFP-tagged) - Mouse ATP-binding cassette sub-family A (ABC1) member 5 (Abca5), (10ug)

Product Images

Circular map for MG222272

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Abca5
• Synonyms: ABC13; B930033A02Rik; mKIAA1888
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222272 representing NM_147219
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACTGCAATTAGGGATGTGGGAGTTTGGAGACAGACCAGAACACTTCTACTGAAAAATTACCTAA
  TTAAATGCAGGACTAAAAAAAGTAGTGTTCAGGAAATTCTTTTTCCTCTATTTTTCTTATTTTGGCTGAT
  ATTAGTTAGCATGATGCATCCAAATAAGAAATATGAAGAGGTATCTGATATAGAGCTCAGCCCTATGGAC
  AAATTCAGCCTTTCCAACGTTATTCTTGGATACACTCCCGTGACTAACATTACAAGCAGCATTATGCAGA
  GGGTTTCTACCGATCATCTTCCCAAGGTTATAGTTACTGAAGAATACGCAAATGAGAAAGAACTGGTAGC
  CGCAAGTCTTTCTAAGTCCAGCAACTTCGTAGGTGTGGTTTTCAAAGACACCATGTCCTATGAACTTCGT
  TTTTTTCCTGAAATGATTCCAGTGTCTTCTATTTATATGAATTCAAGAGAAGGCTGTTCAAAGACATGTG
  ATGCTGCTCAGTACTGGTCTTTGGGGTTTACAGTTCTGCAGGCATCGATAGATGCTGCCATTATACAGCT
  GAAGACCAATGTTTCTGTGTGGAGCGAGCTGGAGTCGACCAAAGCTGTGATCATGGGAGAGGCCGCTGTT
  GTGGAGATTGACACCTTCCCGCGAGGGGTCATCCTCATCTACCTCGTCATAGCCTTCTCGCCCTTCGGCT
  ACTTCCTGGCAATCCACATCGTGGCAGAAAAAGAAAAGAAGTTAAAGGAATTTTTAAAGATAATGGGACT
  TCATGACACTGCTTTTTGGCTTTCCTGGGTTCTTCTGTACGCAAGCTTGATTTTCCTTATGTCCCTGCTT
  ATGGCTGTCATCGCAACAGCTTCTTCGTTATTCCCTCAGAGTAGCAGCATTGTGATCTTTCTACTGTTCT
  TCTTATATGGATTGTCATCTGTGTTTTTTGCTTTAATGCTGACGCCTCTTTTTAAAAAATCAAAACACGT
  GGGAGTCGTTGAGTTTTTTGTCACCGTGGTGTTTGGATTTGTTGGCCTGCTGATTGTCCTCATAGAAAGT
  TTCCCCAGGTCGCTGGTGTGGCTCTTCAGTCCTTTGTGTCAGTGTGCCTTTCTGATTGGGATTGCACAGG
  TCATGCATTTAGAAGATTTTAATGAGGGTGCCTTATTTTCTAATTTGACTGAAGGTCCCTATCCTCTAAT
  TATTACTATTATCATGCTAGCTCTTGACAGTGTGTTCTATGTCCTCCTGGCTGTGTATCTCGACCAAGTC
  ATTCCAGGGGAATTTGGCTTGAGGAGGTCATCTTTGTATTTTTTGAAGCCATCGTATTGGTCAAAAAACA
  AAAGAAACTATAAGGAGCTATCGGAGGGCAACATTAATGGCAATATTAGTCTCAATGAAATTGTTGAGCC
  CGTTTCTTCAGAATTTATAGGGAAAGAAGCTATAAGAATAAGTGGTATTCAGAAATCCTATAGAAAGAAA
  ACTGAGAACGTGGAGGCTTTGAGAAATTTGTCATTTGACATATATGAAGGTCAGATTACTGCACTGCTGG
  GCCACAGTGGAACAGGAAAAAGCACACTGATGAATATTCTGTGTGGACTGTGTCCACCCTCTGATGGGTT
  TGCTTCTATATATGGACACAGAGTCTCTGAAATAGATGAAATGTTTGAAGCAAGAAAAATGATTGGCATA
  TGTCCGCAGTCAGATATAAACTTTGATGTTCTGACAGTAGAAGAAAATTTATCAATTTTGGCTTCAATCA
  AAGGAATACCAGCCAACAATATAATTCAAGAAGTGCAGAAAGTGTTGCTGGATCTGGACATGCAAGCCAT
  CAAAGATAATCAAGCGAAAAAGTTAAGCGGTGGTCAGAAAAGGAAGCTGTCTGTAGGAATTGCAGTTCTC
  GGGAATCCAAAGATACTCCTGCTAGACGAGCCTACAGCAGGAATGGACCCCTGCTCTCGCCATATTGTTT
  GGAATCTTCTAAAGTATAGAAAGGCTAACAGAGTGACCGTGTTTAGTACTCACTTCATGGATGAGGCTGA
  CATTCTTGCCGACAGGAAAGCTGTCATATCACAAGGAATGCTGAAGTGTGTTGGTTCTTCAATTTTCCTA
  AAGAGTAAATGGGGAATCGGCTACCGCCTGAGCATGTATATAGACAGATACTGTGCCACAGAGTCACTGT
  CGTCGCTGGTTAGGCAGCACATACCCGCAGCCGCGCTACTGCAGCAGAATGACCAGCAGCTCGTGTACAG
  CCTGCCTTTCAAAGACATGGACAAATTTTCAGGCTTGTTTTCTGCTCTAGACATTCATTCAAACCTGGGT
  GTTATTTCTTATGGTGTTTCCATGACAACATTGGAAGATGTATTCTTAAAGCTAGAAGTTGAAGCAGAAA
  TTGACCAAGCAGACTATAGTGTATTTACACAGCAGCCGCGGGAGGAAGAAACAGATTCAAAATCCTTTGA
  TGAAATGGAACAGAGTTTACTTATTCTCTCTGAAACGAAAGCTTCTTCAGTGAGCACCATGAGCCTCTGG
  AAGCAGCAAGTGTCTACGATTGCAAAGTTTCATTTCCTCTCATTGAAACGAGAAAGCAAATCAGTGCGCG
  CTGTGTTGCTTCTGCTTTTAATTTTTTTTGCAGTTCAGATTTTTATGTTTTTTCTCCATCATTCTTTTAA
  AAATGCTGTGGTTCCCATCAAACTCGTTCCAGACTTGTATTTCCTAAAGCCTGGAGATAAACCTCATAAA
  TACAAAACAAGCCTGCTGCTTCAAAATTCTACTGACTCAGATATCAATGGTCTTATTGAGTTTTTTGCAC
  ACCAGAACATAATGGTGGCAATGTTTAATGACAGTGACTATGTGTCTGCTGCTCCTCACAGTGCGGCTCT
  CAATGTGGTGCGTTCTGAAAAGGACTATGTTTTTTCTGCTGTTTTCAACAGTACTATGGTTTATTGTTTG
  CCAGTCATGATGAACATCATTAGTAACTACTATCTTTATCATTTAAATGTGACTGAAGCCATCCAGACCT
  GGAGTACCCCGTTCATTCAAGAAATTACTGACATTGTTTTTAAAATTGAGCTATATTTTCAAGCAGCTTT
  GCTTGGAATCATTGTTACTGCAATGCCACCTTACTTTGCCATGGAAAATGCAGAGAATCATAAGATCAAA
  GCTTATACTCAACTTAAACTTTCGGGTCTTTTGCCATCAGCCTATTGGGTTGGACAAGCTGTTGTGGATA
  TTCCCTTGTTTTTTGTTGTTCTGATTTTGATGCTGGGAAGTTTATTTGCATTTCATCATGGACTGTATTT
  TTATCCTGCAAAATTTCTTGCTGTGGTGTTTTGCCTCATTGCTTATGTGCCCTCCGTCATTCTGTTCACG
  TACATAGCTTCGTTCACTTTCAAGAAAATTTTAAATACCAAGGAATTTTGGTCATTTATCTATTCTGTGA
  CCGCATTGGCTTGTGTGGCAATCACGGAAACAACTTTCTTTCTGCAATATGCAGTTACGGCTGTCTTTCA
  TTACACCTTCTGCATAGCCATTCCAATCTACCCTCTCCTGGGTTGTCTGATTTCTTTCATAAAGGGTTCT
  TGGAAGAATATGCCAAAAAATGAGAATACCTACAATCCCTGGGATAGACTTTTAGTTGCTGTAATCATGC
  CCTACCTGCAGTGTATACTGTGGATTTTCCTCTTACAACACTATGAGAAAATACATGGAGGCAGATCTAT
  AAGAAAGGATCCATTTTTCAGGGCCCTTTCACAAAAGGCCAAAAATAAGAAGTTTCCAGAACCACCCATC
  AATGAGGATGAAGATGAAGATGTCAAAGCTGAAAGACTGAAGGTTAAAGAGCTGATGGGTTGCCAGTGTT
  GTGAGGAGAAGCCAGCCATTATGGTATGCAATTTGCATAAAGAGTATGATGACAAGAAAGATTTTCTTCA
  TTCAAGAAAAACAACAAAAGTAGCAACAAAATACATCTCTTTCTGTGTGAAAAAAGGAGAGATCTTGGGA
  CTGTTGGGTCCAAATGGAGCTGGCAAAAGCACAGTCATTAACACTCTGGTTGGGGACGTTGAGCCAACTT
  CAGGCAAGATCTTTCTAGGAGATTATGGTTCACATTCAAGTGAAGATGACGAATCCATTAAGTGTATGGG
  GTACTGCCCCCAGACAAACCCACTGTGGCCAGATCTCACTCTACAGGAACATTTTGAGATTTACGGAGCT
  GTGAAAGGAATGAGTCCTGGTGACATGAAGGAAGTCATCAGTCGAATAACAAAAGCCCTTGATTTGAAAG
  AACATCTTCAGAAGACTGTAAAGAAGCTACCTGCAGGGATCAAGCGAAAGCTGTGTTTTGCTCTCAGCAT
  GCTGGGGAACCCTCAGGTGACGCTGCTGGATGAGCCGTCCACAGGCATGGACCCAAGAGCCAAGCAGCAC
  ATGTGGAGAGCTATTCGAACTGCATTTAAAAACAAAAAGCGGGCCGCCCTCCTGACTACTCATTACATGG
  AAGAGGCAGAGGCTGTCTGTGACAGAGTGGCCATCATGGTATCTGGGCAGCTAAGATGTATTGGAACAGT
  ACAACATCTAAAGAGTAAGTTTGGTAAAGGCTACTTTTTGGAAATTAAATTAAAGGACTGGATAGAAAAC
  CTGGAAATAGATCGCCTTCAAAGAGAAATTCAATATATTTTCCCAAATGCAAGCCGCCAAGAGAGCTTTT
  CTTCTATTTTGGCTTTTAAAATTCCAAAAGAAGATGTTCAGTCTCTCTCACAATCCTTCGCTAAGTTGGA
  AGAAGCAAAACGCACTTTTGCCATTGAAGAATACAGCTTTTCTCAAGCAACCCTGGAACAGGTTTTTGTA
  GAACTTACTAAAGAGCAAGAGGAAGAAGATAACAGCTGTGGGACTTTGGCCAGCACTCTCTGGTGGGAAA
  GGACGCAGGAGGATAGAGTAGTATTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222272 representing NM_147219
  Red=Cloning site Green=Tags(s)
  
  MATAIRDVGVWRQTRTLLLKNYLIKCRTKKSSVQEILFPLFFLFWLILVSMMHPNKKYEEVSDIELSPMD
  KFSLSNVILGYTPVTNITSSIMQRVSTDHLPKVIVTEEYANEKELVAASLSKSSNFVGVVFKDTMSYELR
  FFPEMIPVSSIYMNSREGCSKTCDAAQYWSLGFTVLQASIDAAIIQLKTNVSVWSELESTKAVIMGEAAV
  VEIDTFPRGVILIYLVIAFSPFGYFLAIHIVAEKEKKLKEFLKIMGLHDTAFWLSWVLLYASLIFLMSLL
  MAVIATASSLFPQSSSIVIFLLFFLYGLSSVFFALMLTPLFKKSKHVGVVEFFVTVVFGFVGLLIVLIES
  FPRSLVWLFSPLCQCAFLIGIAQVMHLEDFNEGALFSNLTEGPYPLIITIIMLALDSVFYVLLAVYLDQV
  IPGEFGLRRSSLYFLKPSYWSKNKRNYKELSEGNINGNISLNEIVEPVSSEFIGKEAIRISGIQKSYRKK
  TENVEALRNLSFDIYEGQITALLGHSGTGKSTLMNILCGLCPPSDGFASIYGHRVSEIDEMFEARKMIGI
  CPQSDINFDVLTVEENLSILASIKGIPANNIIQEVQKVLLDLDMQAIKDNQAKKLSGGQKRKLSVGIAVL
  GNPKILLLDEPTAGMDPCSRHIVWNLLKYRKANRVTVFSTHFMDEADILADRKAVISQGMLKCVGSSIFL
  KSKWGIGYRLSMYIDRYCATESLSSLVRQHIPAAALLQQNDQQLVYSLPFKDMDKFSGLFSALDIHSNLG
  VISYGVSMTTLEDVFLKLEVEAEIDQADYSVFTQQPREEETDSKSFDEMEQSLLILSETKASSVSTMSLW
  KQQVSTIAKFHFLSLKRESKSVRAVLLLLLIFFAVQIFMFFLHHSFKNAVVPIKLVPDLYFLKPGDKPHK
  YKTSLLLQNSTDSDINGLIEFFAHQNIMVAMFNDSDYVSAAPHSAALNVVRSEKDYVFSAVFNSTMVYCL
  PVMMNIISNYYLYHLNVTEAIQTWSTPFIQEITDIVFKIELYFQAALLGIIVTAMPPYFAMENAENHKIK
  AYTQLKLSGLLPSAYWVGQAVVDIPLFFVVLILMLGSLFAFHHGLYFYPAKFLAVVFCLIAYVPSVILFT
  YIASFTFKKILNTKEFWSFIYSVTALACVAITETTFFLQYAVTAVFHYTFCIAIPIYPLLGCLISFIKGS
  WKNMPKNENTYNPWDRLLVAVIMPYLQCILWIFLLQHYEKIHGGRSIRKDPFFRALSQKAKNKKFPEPPI
  NEDEDEDVKAERLKVKELMGCQCCEEKPAIMVCNLHKEYDDKKDFLHSRKTTKVATKYISFCVKKGEILG
  LLGPNGAGKSTVINTLVGDVEPTSGKIFLGDYGSHSSEDDESIKCMGYCPQTNPLWPDLTLQEHFEIYGA
  VKGMSPGDMKEVISRITKALDLKEHLQKTVKKLPAGIKRKLCFALSMLGNPQVTLLDEPSTGMDPRAKQH
  MWRAIRTAFKNKKRAALLTTHYMEEAEAVCDRVAIMVSGQLRCIGTVQHLKSKFGKGYFLEIKLKDWIEN
  LEIDRLQREIQYIFPNASRQESFSSILAFKIPKEDVQSLSQSFAKLEEAKRTFAIEEYSFSQATLEQVFV
  ELTKEQEEEDNSCGTLASTLWWERTQEDRVVF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_147219
• ORF Size: 4926 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_147219.2, NP_671752.2
• RefSeq Size: 8339 bp
• RefSeq ORF: 4929 bp
• Locus ID: 217265
• UniProt ID: Q8K448
• Cytogenetics: 11 E1
• Gene Summary: May play a role in the processing of autolysosomes.[UniProtKB/Swiss-Prot Function]