BC029081 Mouse Tagged ORF Clone

CAT#: MG200902

  • TrueORF®

(tGFP-tagged) - Mouse mRNA similar to RIKEN cDNA 1600014C10 gene (cDNA clone MGC:27922 IMAGE:3582676)

ORF Plasmid: DDK tGFP


  "BC029081" in other vectors (3)

Reconstitution Protocol

USD 365.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
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Mouse monoclonal turboGFP antibody, clone OTI2H8
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG200902 representing BC029081
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCATCATGGTAGATGACGTCATGAGGCTTCTGTGCTCCATTTCCCAGGAGAGGAAGATGAAGGCGG
CCGTCAAGCACTCTGGAAAGGGTGCCATGGTTGCGGGGGCAATGGCCTTCGTCGGTGGCTTGGTTGGTGG
CCCACCAGGAATAGCTGTTGGGGGGCTCGTTGGGGGCCTGATAGGTGCTTGGATGACGAGTGGACAGTTC
AAGCCCATTCCTCAGATCTTAATGGAGCTGCCCCCCGCTGAAAAGCAGAAACTTGTTAACCAAGTCATGG
CCATCATCAGGCACTTGGACTGGACAGATGCTGTGCAGCTGACTGCTCTGGTCATGAGCAACCAGGCCAT
GCAGCAGAGGCTCTTGGCCATACTAACGAGCTATGTCACCAAGGAGCTGAAGGCTAAGATCTGCTATGGT
GAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG200902 representing BC029081
Red=Cloning site Green=Tags(s)

MSIMVDDVMRLLCSISQERKMKAAVKHSGKGAMVAGAMAFVGGLVGGPPGIAVGGLVGGLIGAWMTSGQF
KPIPQILMELPPAEKQKLVNQVMAIIRHLDWTDAVQLTALVMSNQAMQQRLLAILTSYVTKELKAKICYG
D

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC029081
ORF Size 425 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC029081
RefSeq Size 1131 bp
RefSeq ORF 425 bp

Other Versions

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.