Dhh (BC062810) Mouse Tagged ORF Clone

CAT#: MG200860

  • TrueORF®

(tGFP-tagged) - Mouse cDNA clone MGC:73610 IMAGE:639130

ORF Plasmid: DDK tGFP

AAV Particle: DDK


  "BC062810" in other vectors (3)

Reconstitution Protocol

USD 350.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (4)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol Dhh
Synonyms C78960
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG200860 representing BC062810
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCTGCCGGCCAGTCTGTTGCCCCTGTGCTGCTTGGCACTCTTGGCACTATCTGCCCAGAGCTGCG
GGCCGGGCCGAGGACCGGTTGGCCGGCGGCGTTATGTGCGCAAGCAACTTGTGCCTCTGCTATACAAGCA
GTTTGTGCCCAGTATGCCCGAGCGGACCCTGGGCGCGAGTGGGCCAGCGGAGGGGAGGGTAACAAGGGGG
TCGGAGCGCTTCCGGGACCTCGTACCCAACTACAACCCCGACATAATCTTCAAGGATGAGGAGAACAGCG
GCGCAGACCGCCTGATGACAGAGGTAAGGAGAGTTTCTCCCCACCTGCTCTACCACACCACCAGCTCCTC
AATTTTCCTACAACAACCCTGGAGGATGAACTGTTCCTTCATCCCCAGAGACCCTAAACTAAAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG200860 representing BC062810
Red=Cloning site Green=Tags(s)

MALPASLLPLCCLALLALSAQSCGPGRGPVGRRRYVRKQLVPLLYKQFVPSMPERTLGASGPAEGRVTRG
SERFRDLVPNYNPDIIFKDEENSGADRLMTEVRRVSPHLLYHTTSSSIFLQQPWRMNCSFIPRDPKLK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC062810
ORF Size 416 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC062810
RefSeq Size 717 bp
RefSeq ORF 416 bp
Locus ID 13363
Cytogenetics 15 55.05 cM
Gene Summary Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.[UniProtKB/Swiss-Prot Function]

Other Versions

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.