Marligen’s Vantage Total RNA Purification Kit provides a rapid method for the isolation and purification of high quality total RNA from a variety of different sample types without the use of phenol or chloroform.
Product features:
Spin-column format allows processing of multiple samples in less than 20 minutes
Isolates all RNA species including large (e.g. mRNA) and LMW RNAs (e.g. miRNAs, siRNAs)
Excellent yields from a variety of sample types Total RNA Yields from the Vantage™ Total RNA Purification Kit
Cells
Hepatocytes
1 x 106
30 µg
NIH/3T3 Cells
5x105 cells
4.4 µg (nanodrop)
Serum or plasma
100 µL
200-300 ng
Stem Cells
50,000
2 µg
HeLa
1 x 106
15 µg
MCF-7 cells
1 x 106
10 µg
E. coli
1 x 106
50 µg
S. cerevisiae
1 x 106
30 µg
Tissue
Brain
10 mg
8 µg
Kidney
10 mg
30 µg
Liver
10 mg
30 µg
Lung
10 mg
10 µg
Spleen
10 mg
35 µg
Comparison of RT-PCR products generated from total RNA purified using Marligen’s kit, a leading competitor's kits and the Trizol extraction method.
Total RNA can be isolated from small amounts of sample – even from a single cell.
Total RNA can be purified from a diverse array of sample types including cultured cells, tissues, blood, bacteria, yeast, fungi, plants, and nasal or throat swabs.