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OriGene shRNA in recent publications
Suppression of Sproutys Has a Therapeutic Effect for a Mouse Model of Ischemia by Enhancing Angiogenesis PLoS ONE. 2009; 4(5): e5467 [Spry4(in vivo application)]

RNA interference-mediated in vivo silencing of FasL as a strategy for the enhancement of DNA vaccine potency Human Gene Therapy. August 2008, 19(8): 763-773 [sh-Luc(in vivo application)]

ZIP4 Regulates Pancreatic Cancer Cell Growth by Activating IL-6/STAT3 Pathway through Zinc Finger Transcription Factor CREB Clin. Cancer Res., Mar 2010; 16: 1423 - 1430 [ZIP4]

The Elk-1 and Serum Response Factor Binding Sites in the Major Immediate-Early Promoter of the Human Cytomegalovirus are Required for Efficient Viral Replication in Quiescent Cells and Compensate for Inactivation of the NF-{kappa}B Sites in Proliferating Cells J. Virol., Feb 2010; 10.1128/JVI.02141-09 [pRS]

MicroRNA Multiplex Detection Assays

Luminex Logo

Developed for Luminex xMAP®, Vantage™ microRNA Multiplex Detection Assays offer researchers a fast and simple method for profiling expression levels of up to 100 microRNAs in hundreds of samples in just a few hours. This cost effective method measures the expression levels of multiple microRNAs for as low as $1/miRNA. Learn more >>

Advantages:

  • xMAP® multiplex bead array allows analysis of up to 100 microRNAs in one sample
  • 96-well format allows for hundreds of samples profiled in one day
  • Simple hybridization protocol can be completed in less than 3 hours
  • Detects microRNA profiles in all types of RNA samples including enriched or degraded samples*
  • All multiplexes include internal assay control for sample normalization

Comparison of methods for detecting microRNA expression levels

 Vantage™ Detection AssaysMicroarraysqRT-PCR
Multiplexyesyesno
Sample throughputhighlowmedium
Flexible assay formatyesnoyes
RT Stepnonoyes
Amplification nonoyes
<4 hour protocolyesnoyes

Products required for a successful microRNA multiplex assay
top
1
Total RNA
Purification Kit
2
MicroRNA
Labeling Kit
3
MicroRNA Panels
or Custom Panels
4
MicroRNA
Detection Kit


 

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