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MicroRNA Expression Plasmid

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MicroRNA Processing Diagram

MicroRNAs (miRNAs) are naturally occurring non-coding RNAs of 21-23 nt and they have been implicated in many biological processes and disease development. MiRNA are processed in multiple steps:

DNA→ Pri-miRNA → Pre-miRNA→ Mature miRNA

OriGene provides clones for over-expression of miRNA of your choice. A region of ~600bp region encompassing each miRNA is cloned downstream of a CMV promoter. Upon transfection, the cellular machinery will process the CMV-driven expression of miRNA precursor into mature miRNA and cellular function can be analyzed.

  • Genome wide miRNA coverage (miRBase release 20.0)
    - 1829 human, 1164 mouse and 436 rat
  • GFP as reporter for transfection monitoring
  • Neomycin selection marker for stable cell establishment
  • 10 μg transfection-ready DNA using PowerPrep® HP Kits
  • 3'-UTR reporter clones available for miRNA target validation
  • Cloning Vector: pCMV-MIR
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Bulk Order Options

Catalog No. Description Price List  
SC410001 Mouse miRNA expression plasmid set (486 vectors, 10ug each in 2-D bar coded tubes) $36000 List shopping cart
SC420001 Human miRNA expression plasmid set (646 vectors, 10ug each in 2-D bar coded tubes) $48000 List shopping cart
SC410002 Mouse miRNA expression plasmid set (486 vectors, 2ug each in 96-well plates) $18000 List shopping cart
SC420002 Human miRNA expression plasmid set (646 vectors, 2ug each in 96-well plates) $24000 List shopping cart

> 10 miRNA plasmids $250 each
> 30 miRNA plasmids $220 each
> 50 miRNA plasmids $200 each
> 100 miRNA plasmids $180 each
Please use “miBULK” as the quote number when place order.

 

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