c-Jun and Ets2 Proteins Regulate Expression of Spleen Tyrosine Kinase in T Cells
J. Biol. Chem., Apr 2012; 287: 11833 - 11841.
[JUND
]
CD16 Regulates TRIF-Dependent TLR4 Response in Human Monocytes and Their Subsets
J. Immunol., Apr 2012; 188: 3584 - 3593.
[FCGR3A
]
Constitutive Expression of Inter-a-inhibitor (IaI) Family Proteins and Tumor Necrosis Factor-stimulated Gene-6 (TSG-6) by Human Amniotic Membrane Epithelial and Stromal Cells Supporting Formation of the Heavy Chain-Hyaluronan (HC-HA) Complex
J. Biol. Chem., Apr 2012; 287: 12433 - 12444.
[TNFAIP6
]
Developmental Endothelial Locus-1 (Del-1) Mediates Clearance of Platelet Microparticles by the Endothelium
Circulation, Apr 2012; 125: 1664 - 1672.
[EDIL3
]
MicroRNAs (miRNAs) are naturally occurring non-coding RNAs of 21-23 nt and they have been implicated in many biological processes and disease development. MiRNA are processed in multiple steps:
DNA→ Pri-miRNA → Pre-miRNA→ Mature miRNA
OriGene provides clones for over-expression of miRNA of your choice. A region of ~600bp region encompassing each miRNA is cloned downstream of a CMV promoter. Upon transfection, the cellular machinery will process the CMV-driven expression of miRNA precursor into mature miRNA and cellular function can be analyzed.
Genome wide miRNA coverage (miRBase release 13.0) - 652 human and 486 mouse
GFP as reporter for transfection monitoring
Neomycin selection marker for stable cell establishment
10 μg transfection-ready DNA using PowerPrep® HP Kits