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Over 1000 citations of OriGene cDNA clones
c-Jun and Ets2 Proteins Regulate Expression of Spleen Tyrosine Kinase in T Cells J. Biol. Chem., Apr 2012; 287: 11833 - 11841. [JUND ]

CD16 Regulates TRIF-Dependent TLR4 Response in Human Monocytes and Their Subsets J. Immunol., Apr 2012; 188: 3584 - 3593. [FCGR3A ]

Constitutive Expression of Inter-a-inhibitor (IaI) Family Proteins and Tumor Necrosis Factor-stimulated Gene-6 (TSG-6) by Human Amniotic Membrane Epithelial and Stromal Cells Supporting Formation of the Heavy Chain-Hyaluronan (HC-HA) Complex J. Biol. Chem., Apr 2012; 287: 12433 - 12444. [TNFAIP6 ]

Developmental Endothelial Locus-1 (Del-1) Mediates Clearance of Platelet Microparticles by the Endothelium Circulation, Apr 2012; 125: 1664 - 1672. [EDIL3 ]

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MicroRNA Expression Plasmid

MicroRNA Processing Diagram

MicroRNAs (miRNAs) are naturally occurring non-coding RNAs of 21-23 nt and they have been implicated in many biological processes and disease development. MiRNA are processed in multiple steps:

DNA→ Pri-miRNA → Pre-miRNA→ Mature miRNA

OriGene provides clones for over-expression of miRNA of your choice. A region of ~600bp region encompassing each miRNA is cloned downstream of a CMV promoter. Upon transfection, the cellular machinery will process the CMV-driven expression of miRNA precursor into mature miRNA and cellular function can be analyzed.

  • Genome wide miRNA coverage (miRBase release 13.0)
    - 652 human and 486 mouse
  • GFP as reporter for transfection monitoring
  • Neomycin selection marker for stable cell establishment
  • 10 μg transfection-ready DNA using PowerPrep® HP Kits
  • 3'-UTR reporter clones available for miRNA target validation
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Bulk Order Options

Catalog No. Description Price  
SC410001 Mouse miRNA expression plasmid set (486 vectors, 10ug each in 2-D bar coded tubes) $36,000 shopping cart
SC420001 Human miRNA expression plasmid set (652 vectors, 10ug each in 2-D bar coded tubes) $48,000 shopping cart
SC410002 Mouse miRNA expression plasmid set (486 vectors, 2ug each in 96-well plates) $18,000 shopping cart
SC420002 Human miRNA expression plasmid set (652 vectors, 2ug each in 96-well plates) $24,000 shopping cart

> 10 miRNA plasmids $250 each
> 30 miRNA plasmids $220 each
> 50 miRNA plasmids $200 each
> 100 miRNA plasmids $180 each
Please use “miBULK” as the quote number when place order.
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Useful Links

pCMV-MIR Vector Validation Data Product Manual FAQ


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