YPEL5 (NM_001127401) Human Mass Spec Standard

CAT#: PH325061

YPEL5 MS Standard C13 and N15-labeled recombinant protein (NP_001120873)

Product Images

Coomassie blue staining of purified YPEL5 protein (Cat# TP325061). The protein was produced from HEK293T cells transfected with YPEL5 cDNA clone (Cat# RC225061) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC225061
• Predicted MW: 13.8 kDa
• Protein Sequence:
  Sequence Link

  >RC225061 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGRIFLDHIGGTRLFSCANCDTILTNRSELISTRFTGATGRAFLFNKVVNLQYSEVQDRVMLTGRHMVRD
  VSCKNCNSKLGWIYEFATEDSQRYKEGRVILERALVRESEGFEEHVPSDNS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001120873
• RefSeq Size: 2639
• RefSeq ORF: 363
• Synonyms: CGI-127
• Locus ID: 51646
• UniProt ID: P62699
• Cytogenetics: 2p23.1
• Summary: Component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (PubMed:29911972). Required for normal cell proliferation (By similarity).[UniProtKB/Swiss-Prot Function]