UBA52 (NM_003333) Human Mass Spec Standard

CAT#: PH311036

UBA52 MS Standard C13 and N15-labeled recombinant protein (NP_003324)

Product Images

Coomassie blue staining of purified UBA52 protein (Cat# TP311036). The protein was produced from HEK293T cells transfected with UBA52 cDNA clone (Cat# RC211036) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC211036
• Predicted MW: 14.5 kDa
• Protein Sequence:
  Sequence Link

  >RC211036 representing NM_003333
  Red=Cloning site Green=Tags(s)
  
  MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
  LRLRGGIIEPSLRQLAQKYNCDKMICRKCYARLHPRAVNCRKKKCGHTNNLRPKKKVK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_003324
• RefSeq Size: 2801
• RefSeq ORF: 384
• Synonyms: CEP52; HUBCEP52; L40; RPL40
• Locus ID: 7311
• UniProt ID: P62987, Q3MIH3, Q7Z4P3
• Cytogenetics: 19p13.11
• Summary: Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Ribosome