Josephin 2 (JOSD2) (NM_138334) Human Mass Spec Standard

CAT#: PH309025

JOSD2 MS Standard C13 and N15-labeled recombinant protein (NP_612207)

Product Images

Coomassie blue staining of purified JOSD2 protein (Cat# TP309025). The protein was produced from HEK293T cells transfected with JOSD2 cDNA clone (Cat# RC209025) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC209025
• Predicted MW: 20.8 kDa
• Protein Sequence:
  Sequence Link

  >RC209025 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSQAPGAQPSPPTVYHERQRLELCAVHALNNVLQQQLFSQEAADEICKRLAPDSRLNPHRSLLGTGNYDV
  NVIMAALQGLGLAAVWWDRRRPLSQLALPQVLGLILNLPSPVSLGLLSLPLRRRHWVALRQVDGVYYNLD
  SKLRAPEALGDEDGVRAFLAAALAQGLCEVLLVVTKEVEEKGSWLRTD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_612207
• RefSeq Size: 863
• RefSeq ORF: 564
• Synonyms: SBBI54
• Locus ID: 126119
• UniProt ID: Q8TAC2, A0A024R4G2
• Cytogenetics: 19q13.33
• Summary: This gene encodes a protein containing a Josephin domain. Josephin domain-containing proteins are deubiquitinating enzymes which catalyze the hydrolysis of the bond between the C-terminal glycine of the ubiquitin peptide and protein substrates. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
• Protein Families: Druggable Genome