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OriGene & ISB Alliance to Create a SRM/MRM Standard Database for 5,000 Human Proteins

Rockville, MD/Seattle, WA ?May 11, 2010 ?OriGene and the Institute for Systems Biology (ISB), announce plans to create a proteotypic PeptideAtlas and develop an SRM/MRM mass spectrometry standard database for 5,000 human proteins (Press Release)

Dr. Leroy Hood on Proteome Project at OriGene's ASMS2010 Luncheon Dr. Leroy Hood Presentation on ASMS 2010

Dr. Wei-wu He on Development of Assays for Every Human Protein Dr. Weiwu He Presentation on ASMS 2010

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Heavy-labeled Full-length Protein as MS Standards

Accurate quantification of human protein biomarkers

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Mass Spec Validation by ISB
OriGene recombinant proteins are validated by MS/MS through a collaboration with The Institute for Systems Biology. View experiment flow and results.

OriGene now offers the service to generate Mass Spectrometry (MS) standards for 5,000 human proteins. Produced in human HEK293T cells and labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine, the full length proteins with appropriate post-translational modifications are the best identification and quantification standards.

Supriority of OriGene’s MS standards:

  • Spiking at the early stage of sample process for accurate quantification
  • Identify the best SRM and MRM transitions through experimental data
    • Webinar
    John Koomen View audio slideshow from Dr. John Koomen (Moffit Cancer Center) spacer
    Internal standards of quantitative MS in cancer biology
  • Authentic posttranslational modifications by using human HEK293T cell line.
  • Higher data consistency than synthetic peptide internal standard
  • Suitable for all types of MS equipments
  • Over 90% incorporation efficiency. Learn more...

Comparison of OriGene protein based MS Internal Standard Vs. QconCAT or AQUA peptide based MS internal standard

  OriGene AQUA QconCat
Reproducibility High Low Mid
Identification of best SRM and MRM transitions Yes No No
Post-translational modifications and processing Yes No No
Coverage 5000 100s N/A

Applications - Protein quantification, protein identification & biomarker discovery

MassSpec Standard

Catalog No. Description Price Ship Date
PHxxxxxx Heavy-Labeled Full-length Protein as MS Standard (10ug) $1500 3 weeks
Search for your proteins, typing protein name, symbol, accession number or keyword in the box below, and select Proteins
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Custom MRM Standard Service
If your protein of interest is not offered as a catalog item, take advantage of our custom MRM standard service. Click here to specify your need.

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