BTD (NM_001281723) Human Untagged Clone

CAT#: SC336727

BTD (untagged) - Human biotinidase (BTD), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BTD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336727 representing NM_001281723.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCAGAGGGAGGCGGGACTAGCAGGAGATTGCTGCCTATGCAAAGCAGATTTGTGGTCTGCATTATG
  TCTGGAGCCAGAAGTAAGCTTGCTCTTTTCCTCTGCGGCTGTTACGTGGTTGCCCTGGGAGCCCACACC
  GGGGAGGAGAGCGTGGCTGACCATCACGAGGCTGAATATTATGTGGCTGCCGTGTATGAGCATCCATCC
  ATCCTGAGTCTGAACCCTCTGGCTCTCATCAGCCGCCAAGAGGCCTTGGAGCTCATGAACCAGAACCTT
  GACATCTATGAACAGCAAGTGATGACTGCAGCCCAAAAGGATGTACAGATTATAGTGTTTCCAGAAGAT
  GGCATTCATGGATTCAACTTTACAAGAACATCCATTTATCCATTTTTGGACTTCATGCCGTCTCCCCAG
  GTGGTCAGGTGGAACCCATGCCTGGAGCCTCACCGCTTCAATGACACAGAGGTGCTCCAGCGCCTGAGT
  TGTATGGCCATCAGGGGAGATATGTTCTTGGTGGCCAATCTTGGGACAAAGGAGCCTTGTCATAGCAGT
  GACCCAAGGTGCCCAAAAGATGGGAGATACCAGTTCAACACAAATGTCGTGTTCAGCAATAATGGAACC
  CTTGTTGACCGCTACCGTAAACACAACCTCTACTTTGAGGCAGCATTCGATGTTCCTCTTAAAGTGGAT
  CTCATCACCTTTGATACCCCCTTTGCTGGCAGGTTTGGCATCTTCACATGCTTTGATATATTGTTCTTT
  GACCCTGCCATCAGAGTCCTCAGAGACTACAAGGTGAAGCATGTTGTGTACCCAACTGCCTGGATGAAC
  CAGCTCCCACTCTTGGCAGCAATTGAGATTCAGAAAGCTTTTGCTGTTGCCTTTGGCATCAACGTTCTG
  GCAGCTAATGTCCACCACCCAGTTCTGGGGATGACAGGAAGTGGCATACACACCCCTCTGGAGTCCTTT
  TGGTACCATGACATGGAAAATCCCAAAAGTCACCTTATAATTGCCCAGGTGGCCAAAAATCCAGTGGGT
  CTCATTGGTGCAGAGAATGCAACAGGTGAAACGGACCCATCCCATAGTAAGTTTTTAAAAATTTTGTCA
  GGCGATCCGTACTGTGAGAAGGATGCTCAGGAAGTCCACTGTGATGAGGCCACCAAGTGGAACGTGAAT
  GCTCCTCCCACATTTCACTCTGAGATGATGTATGACAATTTCACCCTGGTCCCTGTCTGGGGAAAGGAA
  GGCTATCTCCACGTCTGTTCCAATGGCCTCTGCTGTTATTTACTTTACGAGAGGCCCACCTTATCCAAA
  GAGCTGTATGCCCTGGGGGTCTTTGATGGGCTTCACACAGTACATGGCACTTACTACATCCAAGTGTGT
  GCCCTGGTCAGGTGTGGGGGTCTTGGCTTCGACACCTGTGGACAGGAAATCACAGAGGCCACGGGGATA
  TTTGAGTTTCACCTGTGGGGCAACTTCAGTACTTCCTATATCTTTCCTTTGTTTCTGACCTCAGGGATG
  ACCCTAGAAGTCCCTGACCAGCTTGGCTGGGAGAATGACCACTATTTCCTGAGGAAAAGTAGGCTGTCC
  TCTGGGCTGGTGACGGCGGCTCTCTATGGGCGCTTGTATGAGAGGGACTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001281723
• Insert Size: 1638 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001281723.2
• RefSeq Size: 4169 bp
• RefSeq ORF: 1638 bp
• Locus ID: 686
• UniProt ID: P43251
• Cytogenetics: 3p25.1
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Biotin metabolism, Metabolic pathways
• MW: 61.3 kDa
• Gene Summary: The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (1) encodes isoform 1. Variants 1-4 and 6 encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.