IL15 (NM_000585) Human Untagged Clone

CAT#: SC324164

IL15 (untagged)-Human interleukin 15 (IL15), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IL15
• Synonyms: IL-15
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000585.2 ACTCCGGGTGGCAGGCGCCCGGGGGAATCCCAGCTGACTCGCTCACTGCCTTCGAAGTCC
  GGCGCCCCCCGGGAGGGAACTGGGTGGCCGCACCCTCCCGGCTGCGGTGGCTGTCGCCCC
  CCACCCTGCAGCCAGGACTCGATGGAGGTACAGAGCTCGGCTTCTTTGCCTTGGGAGGGG
  AGTGGTGGTGGTTGAAAGGGCGATGGAATTTTCCCCGAAAGCCTACGCCCAGGGCCCCTC
  CCAGCTCCAGCGTTACCCTCCGGTCTATCCTACTGGCCGAGCTGCCCCGCCTTCTCATGG
  GGAAAACTTAGCCGCAACTTCAATTTTTGGTTTTTCCTTTAATGACACTTCTGAGGCTCT
  CCTAGCCATCCTCCCGCTTCCGGAGGAGCGCAGATCGCAGGTCCCTTTGCCCCTGGCGTG
  CGACTCCCTACTGCGCTGCGCTCTTACGGCGTTCCAGGCTGCTGGCTAGCGCAAGGCGGG
  CCGGGCACCCCGCGCTCCGCTGGGAGGGTGAGGGACGCGCGTCTGGCGGCCCCAGCCAAG
  CTGCGGGTTTCTGAGAAGACGCTGTCCCGCAGCCCTGAGGGCTGAGTTCTGCACCCAGTC
  AAGCTCAGGAAGGCCAAGAAAAGAATCCATTCCAATATATGGCCATGTGGCTCTTTGGAG
  CAATGTTCCATCATGTTCCATGCTGCTGACGTCACATGGAGCACAGAAATCAATGTTAGC
  AGATAGCCAGCCCATACAAGATCGTATTGTATTGTAGGAGGCATCGTGGATGGATGGCTG
  CTGGAAACCCCTTGCCATAGCCAGCTCTTCTTCAATACTTAAGGATTTACCGTGGCTTTG
  AGTAATGAGAATTTCGAAACCACATTTGAGAAGTATTTCCATCCAGTGCTACTTGTGTTT
  ACTTCTAAACAGTCATTTTCTAACTGAAGCTGGCATTCATGTCTTCATTTTGGGCTGTTT
  CAGTGCAGGGCTTCCTAAAACAGAAGCCAACTGGGTGAATGTAATAAGTGATTTGAAAAA
  AATTGAAGATCTTATTCAATCTATGCATATTGATGCTACTTTATATACGGAAAGTGATGT
  TCACCCCAGTTGCAAAGTAACAGCAATGAAGTGCTTTCTCTTGGAGTTACAAGTTATTTC
  ACTTGAGTCCGGAGATGCAAGTATTCATGATACAGTAGAAAATCTGATCATCCTAGCAAA
  CAACAGTTTGTCTTCTAATGGGAATGTAACAGAATCTGGATGCAAAGAATGTGAGGAACT
  GGAGGAAAAAAATATTAAAGAATTTTTGCAGAGTTTTGTACATATTGTCCAAATGTTCAT
  CAACACTTCTTGATTGCAATTGATTCTTTTTAAAGTGTTTCTGTTATTAACAAACATCAC
  TCTGCTGCTTAGACATAACAAAACACTCGGCATTTCAAATGTGCTGTCAAAACAAGTTTT
  TCTGTCAAGAAGATGATCAGACCTTGGATCAGATGAACTCTTAGAAATGAAGGCAGAAAA
  ATGTCATTGAGTAATATAGTGACTATGAACTTCTCTCAGACTTACTTTACTCATTTTTTT
  AATTTATTATTGAAATTGTACATATTTGTGGAATAATGTAAAATGTTGAATAAAAATATG
  TACAAGTGTTGTTTTTTAAGTTGCACTGATATTTTACCTCTTATTGCAAAATAGCATTTG
  TTTAAGGGTGATAGTCAAATTATGTATTGGTGGGGCTGGGTACCAATGCTGCAGGTCAAC
  AGCTATGCTGGTAGGCTCCTGCCTGTGTGGAACCACTGACTACTGGCTCTCATTGACTTC
  CTTACTAAGCATAGCAAACAGAGGAAGAATTTGTTATCAGTAAGAAAAAGAAGAACTATA
  TGTGAATCCTCTTCTTTACACTGTAATTTAGTTATTGATGTATAAAGCAACTGTTATGAA
  ATAAAGAAATTGCAATAACTGGCAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_000585
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000585.2, NP_000576.1
• RefSeq Size: 1496 bp
• RefSeq ORF: 489 bp
• Locus ID: 3600
• UniProt ID: P40933
• Cytogenetics: 4q31.21
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
• Gene Summary: The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively spliced transcript variants of this gene have been reported. [provided by RefSeq, Feb 2011]
  Transcript Variant: This variant (3) represents the shortest transcript and encodes the longer isoform (1, also known as 48aa(LSP)-IL15).