GGT5 (NM_001099782) Human Untagged Clone

CAT#: SC316775

GGT5 (untagged)-Human gamma-glutamyltransferase 5 (GGT5), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GGT5
• Synonyms: GGL; GGT-REL; GGT 5; GGTLA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC316775 representing NM_001099782.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCCGGGGCTACGGGGCCACGGTCAGCCTAGTCCTGCTGGGTCTGGGGCTGGCGCTGGCTGTCATT
  GTGCTGGCTGTGGTCCTCTCTCGACACCAGGCCCCATGTGGCCCCCAGGCCTTTGCCCACGCTGCTGTT
  GCCGCCGACTCCAAGGTCTGCTCGGATATTGGACGAGCCATCCTCCAGCAGCAGGGCTCACCCGTGGAT
  GCCACCATCGCGGCTCTGGTCTGCACCAGCGTCGTCAACCCTCAGAGCATGGGCCTGGGCGGAGGGGTC
  ATCTTCACCATCTACAATGTGACAACAGGGGCCCAGTGGATCGGGGTGCCCGGGGAGCTCCGTGGCTAT
  GCCGAGGCCCACCGCCGCCATGGCCGCCTGCCCTGGGCGCAGCTGTTCCAGCCCACCATCGCGCTGCTC
  CGAGGGGGGCATGTGGTGGCCCCTGTCCTCAGCCGTTTCCTGCACAACAGCATCCTGCGGCCTTCCTTG
  CAGGCGTCAACCCTGCGCCAGCTCTTCTTCAACGGGACAGAACCCCTGAGGCCTCAGGACCCACTCCCA
  TGGCCTGCACTGGCCACCACCCTGGAGACCGTGGCCACAGAGGGCGTGGAGGTCTTCTACACGGGGAGG
  CTGGGCCAGATGCTGGTGGAGGACATTGCCAAGGAAGGGAGCCAGCTGACGCTGCAGGACCTGGCCAAG
  TTCCAGCCCGAGGTGGTGGATGCCCTGGAGGTGCCCCTGGGGGACTATACCCTGTACTCACCACCGCCG
  CCTGCAGGGGGTGCCATTCTCAGCTTTATCCTCAACGTGCTAAGAGGGTTCAACTTCTCAACAGAGTCT
  ATGGCCAGGCCTGAAGGGAGGGTGAACGTGTACCACCACCTTGTAGAGACGCTCAAGTTTGCCAAGGGG
  CAGAGGTGGAGGCTGGGGGACCCTCGAAGCCACCCGAAGCTCCAGAATGCCTCCCGGGACCTGCTGGGG
  GAGACCCTGGCCCAGCTCATCCGCCAACAGATCGATGGCCGGGGGGACCACCAGCTCAGCCACTACAGC
  TTGGCCGAGGCCTGGGGCCACGGGACAGGCACGTCCCATGTGTCTGTGCTGGGGGAGGATGGCAGCGCC
  GTGGCTGCCACCAGCACCATCAACACACCCTTTGGAGCGATGGTGTATTCACCACGGACAGGCATCATC
  CTCAACAACGAGCTCCTGGACTTATGCGAGCGATGCCCCCGGGGTTCCGGCACCACCCCCTCACCTGTG
  AGTGGAGACAGGGTGGGTGGAGCTCCCGGAAGGTGCTGGCCCCCAGTTCCAGGCGAGCGTTCCCCATCC
  TCCATGGTGCCCTCCATCTTGATCAACAAAGCCCAGGGGTCGAAGCTAGTGATTGGCGGGGCTGGCGGG
  GAGCTCATCATCTCTGCTGTGGCCCAGGCCATCATGAGCAAGCTGTGGCTTGGCTTTGACCTGAGAGCG
  GCCATTGCAGCCCCCATCCTGCATGTCAACAGCAAGGGCTGTGTGGAGTACGAGCCCAACTTCAGCCAG
  GAGGTGCAGAGGGGACTCCAAGACCGTGGCCAGAACCAGACCCAGAGGCCCTTCTTCCTGAACGTGGTC
  CAGGCTGTGTCCCAGGAGGGGGCCTGTGTGTACGCCGTCTCGGACCTGAGGAAGAGTGGGGAGGCCGCA
  GGCTACTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001099782
• Insert Size: 1665 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001099782.2
• RefSeq Size: 2415 bp
• RefSeq ORF: 1665 bp
• Locus ID: 2687
• UniProt ID: P36269
• Cytogenetics: 22q11.23
• Protein Families: Protease, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Cyanoamino acid metabolism, Glutathione metabolism, Metabolic pathways, Selenoamino acid metabolism, Taurine and hypotaurine metabolism
• MW: 59 kDa
• Gene Summary: This gene is a member of the gamma-glutamyl transpeptidase gene family, and some reports indicate that it is capable of cleaving the gamma-glutamyl moiety of glutathione. The protein encoded by this gene is synthesized as a single, catalytically-inactive polypeptide, that is processed post-transcriptionally to form a heavy and light subunit, with the catalytic activity contained within the small subunit. The encoded enzyme is able to convert leukotriene C4 to leukotriene D4, but appears to have distinct substrate specificity compared to gamma-glutamyl transpeptidase. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.