CD39 (ENTPD1) (NM_001776) Human Untagged Clone

CAT#: SC313783

ENTPD1 (untagged)-Human ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD39
• Synonyms: ATPDase; CD39; NTPDase-1; SPG64
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001776 edited
  ATGGAAGATACAAAGGAGTCTAACGTGAAGACATTTTGCTCCAAGAATATCCTAGCCATC
  CTTGGCTTCTCCTCTATCATAGCTGTGATAGCTTTGCTTGCTGTGGGGTTGACCCAGAAC
  AAAGCATTGCCAGAAAACGTTAAGTATGGGATTGTGCTGGATGCGGGTTCTTCTCACACA
  AGTTTATACATCTATAAGTGGCCAGCAGAAAAGGAGAATGACACAGGCGTGGTGCATCAA
  GTAGAAGAATGCAGGGTTAAAGGTCCTGGAATCTCAAAATTTGTTCAGAAAGTAAATGAA
  ATAGGCATTTACCTGACTGATTGCATGGAAAGAGCTAGGGAAGTGATTCCAAGGTCCCAG
  CACCAAGAGACACCCGTTTACCTGGGAGCCACGGCAGGCATGCGGTTGCTCAGGATGGAA
  AGTGAAGAGTTGGCAGACAGGGTTCTGGATGTGGTGGAGAGGAGCCTCAGCAACTACCCC
  TTTGACTTCCAGGGTGCCAGGATCATTACTGGCCAAGAGGAAGGTGCCTATGGCTGGATT
  ACTATCAACTATCTGCTGGGCAAATTCAGTCAGAAAACAAGGTGGTTCAGCATAGTCCCA
  TATGAAACCAATAATCAGGAAACCTTTGGAGCTTTGGACCTTGGGGGAGCCTCTACACAA
  GTCACTTTTGTACCCCAAAACCAGACTATCGAGTCCCCAGATAATGCTCTGCAATTTCGC
  CTCTATGGCAAGGACTACAATGTCTACACACATAGCTTCTTGTGCTATGGGAAGGATCAG
  GCACTCTGGCAGAAACTGGCCAAGGACATTCAGGTTGCAAGTAATGAAATTCTCAGGGAC
  CCATGCTTTCATCCTGGATATAAGAAGGTAGTGAACGTAAGTGACCTTTACAAGACCCCC
  TGCACCAAGAGATTTGAGATGACTCTTCCATTCCAGCAGTTTGAAATCCAGGGTATTGGA
  AACTATCAACAATGCCATCAAAGCATCCTGGAGCTCTTCAACACCAGTTACTGCCCTTAC
  TCCCAGTGTGCCTTCAATGGGATTTTCTTGCCACCACTCCAGGGGGATTTTGGGGCATTT
  TCAGCTTTTTACTTTGTGATGAAGTTTTTAAACTTGACATCAGAGAAAGTCTCTCAGGAA
  AAGGTGACTGAGATGATGAAAAAGTTCTGTGCTCAGCCTTGGGAGGAGATAAAAACATCT
  TACGCTGGAGTAAAGGAGAAGTACCTGAGTGAATACTGCTTTTCTGGTACCTACATTCTC
  TCCCTCCTTCTGCAAGGCTATCATTTCACAGCTGATTCCTGGGAGCACATCCATTTCATT
  GGCAAGATCCAGGGCAGCGACGCCGGCTGGACTTTGGGCTACATGCTGAACCTGACCAAC
  ATGATCCCAGCTGAGCAACCATTGTCCACACCTCTCTCCCACTCCACCTATGTCTTCCTC
  ATGGTTCTATTCTCCCTGGTCCTTTTCACAGTGGCCATCATAGGCTTGCTTATCTTTCAC
  AAGCCTTCATATTTCTGGAAAGATATGGTATAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001776 unedited
  ATTTTGGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGACCACAGCA
  AGCAGAGGCTGGGGGGGGGAAAGACGAGGAAAGAGGAGGAAAACAAAAGCTGCTACTTAT
  GGAAGATACAAAGGAGTCTAACGTGAAGACATTTTGCTCCAAGAATATCCTAGCCATCCT
  TGGCTTCTCCTCTATCATAGCTGTGATAGCTTTGCTTGCTGTGGGGTTGACCCAGAACAA
  AGCATTGCCAGAAAACGTTAAGTATGGGATTGTGCTGGATGCGGGTTCTTCTCACACAAG
  TTTATACATCTATAAGTGGCCAGCAGAAAAGGAGAATGACACAGGCGTGGTGCATCAAGT
  AGAAGAATGCAGGGTTAAAGGTCCTGGAATCTCAAAATTTGTTCAGAAAGTAAATGAAAT
  AGGCATTTACCTGACTGATTGCATGGAAAGAGCTAGGGAAGTGATTCCAAGGTCCCAGCA
  CCAAGAGACACCCGTTTACCTGGGAGCCACGGCAGGCATGCGGTTGCTCAGGATGGAAAG
  TGAAGAGTTGGCAGACAGGGTTCTGGATGTGGTGGAGAGGACTCTTAGCANCTACCCCTT
  TGACTTCCAGGGTGCCAGGATCATTACTGGTCAAGAGGAAGGTGCCTATGGCCTGGATAC
  TACGGGCTGTGCGCCGGGGCACTTCCCCCCCCCCCCCGCGGCCCTCGTGCGCCTTCCCTC
  GCCCGGTCCCCCCCCGATCCCCCCTCCTCGTCCCGCTCCGCGGCTTCCTGCCCGCCCCCT
  CCTCGCGCCGCTCGCGCCCCGCCGCCGCCGCCCCCCGCCCCTATCCCCCCCTCCCCCCCC
  CCCCCTTCCCCGCTTTCAACCTCCTCTGTTACTCCCGCCCCCCCCGCCGTACCCACCTCT
  CCTCCTCGCCCCCGTCTCTCCCCCCCGTCTCCCCTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_001776 unedited
  TGGCCACTGCACTTCAGGGCCGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGG
  GATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTCCCTTTCCACAGCTTTATTAAGTTTTTATATTCACC
  TGGATGTTGTCAAAAGTGACTTGATCATTCAAGAGATAGGGGACATTTGGCTTCCGGTTT
  GTGTGAAAGCTTTTCTTTCCCCATCAGCTCAACAGTCAGTCCCCAAATCTAGAGATGGAG
  AGCTTGGGACAAGGTCAAAATGAAACATCACCAGTCAATCCTGCTCAACAGGTAACTCTT
  GCAGCAATCCTTAAGGAAAGGGGTGCAGGGTCTCTTCATGTTTCCATGGTAAGGTCTATA
  GCTGCTGCTTCATGGAATAAGCAAACAAAGCCCATGTTGTGTGACGGGTGGCAACACTTT
  CAAACTTGGATCCTGCACATTCTGATGTGTAAATTCCACGATAGGGCAAAAGCATGCCAC
  TCCACACTAATGCTGCCCTCTCACTAAATTAAACCTTGGAAAAATAAGATGTGGCTTGGC
  TTTAAAACTCAGTGAACTATCTTTATGGTACAGTTGGTTTCCATAGCTGTAAACACAAAA
  GATCTATGGTTTCTGCCTACCCAGGATAGTCTTGTCTCTAGACAATGTATTGATGATGCA
  AATGTTCTGGAACTAAATAGTGGTGATGGTGGCACAACACTGTGAATGTACTAAATGTCA
  CTGATTTGTACGTATTTAATATGATTAATGTAAAATGGTGAATTATATTTATGTATATTT
  TACCATAATAAAAAATATTGCATTGGAGATGCATTTTTTGGTGACCCTGGAGTTGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001776
• Insert Size: 3500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001776.3, NP_001767.3
• RefSeq Size: 1959 bp
• RefSeq ORF: 1533 bp
• Locus ID: 953
• UniProt ID: P49961
• Cytogenetics: 10q24.1
• Domains: GDA1_CD39
• Protein Families: Transmembrane
• Protein Pathways: Purine metabolism, Pyrimidine metabolism
• Gene Summary: The protein encoded by this gene is a plasma membrane protein that hydrolyzes extracellular ATP and ADP to AMP. Inhibition of this protein's activity may confer anticancer benefits. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.