Dopamine D2 Receptor (DRD2) (NM_016574) Human Untagged Clone

CAT#: SC308892

DRD2 (untagged)-Human dopamine receptor D2 (DRD2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Dopamine D2 Receptor
• Synonyms: D2DR; D2R
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_016574 edited
  GGGCGGCCGCGAATTCGGCACGAGCCGCCCCGCCGCGGCCCGTCCACTGCTCCCCGCGGG
  CCAGAGCCGGCCGAGCTGCTGCCCGCCGGGGCTCTGAACGGCGCGGCGGGGCCGGGAGCC
  AGGGACCGGCCGAGGAGAGTGGCGGCCCCGGACGGCTGCCGGAGGGGCGGCCGCGCGTGG
  ATGCGGCGGGAGCTGGAAGCCTCAAGCAGCCGGCGCCGTCTCTGCCCCGGGGCGCCCTAT
  GGCTTGAAGAGCCTGGCCACCCAGTGGCTCCACCGCCCTGATGGATCCACTGAATCTGTC
  CTGGTATGATGATGATCTGGAGAGGCAGAACTGGAGCCGGCCCTTCAACGGGTCAGACGG
  GAAGGCGGACAGACCCCACTACAACTACTATGCCACACTGCTCACCCTGCTCATCGCTGT
  CATCGTCTTCGGCAACGTGCTGGTGTGCATGGCTGTGTCCCGCGAGAAGGCGCTGCAGAC
  CACCACCAACTACCTGATCGTCAGCCTCGCAGTGGCCGACCTCCTCGTCGCCACACTGGT
  CATGCCCTGGGTTGTCTACCTGGAGGTGGTAGGTGAGTGGAAATTCAGCAGGATTCACTG
  TGACATCTTCGTCACTCTGGACGTCATGATGTGCACGGCGAGCATCCTGAACTTGTGTGC
  CATCAGCATCGACAGGTACACAGCTGTGGCCATGCCCATGCTGTACAATACGCGCTACAG
  CTCCAAGCGCCGGGTCACCGTCATGATCTCCATCGTCTGGGTCCTGTCCTTCACCATCTC
  CTGCCCACTCCTCTTCGGACTCAATAACGCAGACCAGAACGAGTGCATCATTGCCAACCC
  GGCCTTCGTGGTCTACTCCTCCATCGTCTCCTTCTACGTGCCCTTCATTGTCACCCTGCT
  GGTCTACATCAAGATCTACATTGTCCTCCGCAGACGCCGCAAGCGAGTCAACACCAAACG
  CAGCAGCCGAGCTTTCAGGGCCCACCTGAGGGCTCCACTAAAGGAGGCTGCCCGGCGAGC
  CCAGGAGCTGGAGATGGAGATGCTCTCCAGCACCAGCCCACCCGAGAGGACCCGGTACAG
  CCCCATCCCACCCAGCCACCACCAGCTGACTCTCCCCGACCCGTCCCACCACGGTCTCCA
  CAGCACTCCCGACAGCCCCGCCAAACCAGAGAAGAATGGGCATGCCAAAGACCACCCCAA
  GATTGCCAAGATCTTTGAGATCCAGACCATGCCCAATGGCAAAACCCGGACCTCCCTCAA
  GACCATGAGCCGTAGGAAGCTCTCCCAGCAGAAGGAGAAGAAAGCCACTCAGATGCTCGC
  CATTGTTCTCGGCGTGTTCATCATCTGCTGGCTGCCCTTCTTCATCACACACATCCTGAA
  CATACACTGTGACTGCAACATCCCGCCTGTCCTGTACAGCGCCTTCACGTGGCTGGGCTA
  TGTCAACAGCGCCGTGAACCCCATCATCTACACCACCTTCAACATTGAGTTCCGCAAGGC
  CTTCCTGAAGATCCTCCACTGCTGACTCTGCTGCCTGCCCGCACAGCAGCCTGCTTCCCA
  CCTCCCTGCCCAGGCCAGCCAGCCTCACCCTTGCGAACCGTGAGCAGGAAGGCCTGGGTG
  GATCGGCCTCCTCTTCACCCCGGCAGGCCCTGCAGTGTTCGCTTGGCTCCATGCTCCTCA
  CTGCCCGCACACCCTCACTCTGCCAGGGCAGTGCTAGTGAGCTGGGCATGGTACCAGCCC
  TGGGGCTGGGCCCCCCAGCTCAGGGGCAGCTCATAGAGTCCCCCCTCCCACCTCCAGTCC
  CCCTATCCTTGGCACCAAAGATGCAGCCGCCTTCCTTGACCTTCCTCTGGGGCTCTAGGG
  TTGCTGGAGCCTGAGTCAGGGCCCAGAGGCTGAGTTTTCTGTTTGTGGGGCTTGGCGTGG
  AGCAGGCGGTGGGGAGAGATGGACAGTTCACACCCTGCAAGGCCCACAGGAGGCAAGCAA
  GCTCTCTTGCCGAGGAGCCAGGCAACTTCAGTCCTGGGAGACCCATGTAAATACCAGACT
  GCAGGTTGGACCCCAGAGATTCCCAAGCCAAAAACCTTAGCTCCCTCCCGCACCCCGATG
  TGGACCTCTACTTTCCAGGCTAGTCCGGACCCACCTCACCCCGTTACAGCTCCCCAAGTG
  GTTTCCACATGCTCTGAGAAGAGGAGCCCTCATCTTGAAGGGCCCAGGAGGGTCTATGGG
  GAGAGGAACTCCTTGGCCTAGCCCACCCTTCTGCCTTCTGACGGCCCTGCAATGTATCCC
  TTCTCACAGCACATGCTGGCCAGCCTGGGGCCTGGCAGGGAGGTCAGGCCCTGGAACTCT
  ATCTGGGCCTGGGCTAGGGGACATCAGAGGTTCTTTGAGGGACTGCCTCTGCCACACTCT
  GACGCAAAACCACTTTCCTTTTCTATTCCTTCTGGCCTTTCCTCTCTCCTGTTTCCCTTC
  CCTTCCACTGCCTCTGCCTTAGAGGAGCCCACGGCTAAGAGGCTGCTGAAAACCATCTGG
  CCTGGCCTGGCCCTGCCCTGAGGAAGGAGGGGAAGCTGCAGCTTGGGAGAGCCCCTGGGG
  CCTAGACTCTGTAACATCACTATCCATGCACCAAACTAATAAAACTTTGACGAGTCACCT
  CCAGGAAAAAAAAAAAAAAGGGAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016574 unedited
  TTACATCGCCGACCCGTTGTCCCATTGGGCGGTAGGCGTGTGCGGTGGGCAGGTCTATAT
  AAGCAGAGCTCGTTTAGTGAACCGTCAGATTTTGTAATACGACTCACTATAGGGCGGCCG
  CGAATTCGGCACGAGCCGCCCCGCCGCGGCCCGTCCACTGCTCCCCGCGGGCCAGAGCCG
  GCCGAGCTGCTGCCCGCCGGGGCTCTGAACGGCGCGGCGGGGCCGGGAGCCAGGGACCGG
  CCGAGGAGAGTGGCGGCCCCGGACGGCTGCCGGAGGGGCGGCCGCGCGTGGATGCGGCGG
  GAGCTGGAAGCCTCAAGCAGCCGGCGCCGTCTCTGCCCCGGGGCGCCCTATGGCTTGAAG
  AGCCTGGCCACCCAGTGGCTCCACCGCCCTGATGGATCCACTGAATCTGTCCTGGTATGA
  TGATGATCTGGAGAGGCAGAACTGGAGCCGGCCCTTCAACGGGTCATACGGGAAGGCGGA
  CAGACCCCACTACAACTACTATGCCACACTGCTCACCCTGCTCATCGCTGTCATCGTCTT
  CGGCAACGTGCTGGTGTGCATGGCTGTGTCCCGCGAGGAGGCGCTGCAGACCACCACCAA
  CTACCTGATCGTCAGCCTCACAGTGGCCGACCTCCTCGTCGCCACACTGGTCATGCCCTG
  GGTTGTCTACCTGGAAGTGGTAAGTGAGTGGAAAATCAGCAGCATCACTGTGACATCTTC
  ATCACTCCGGGCGTCATGATGTGCACGGGCGAGCCTTCTGAACCTGTGGTGCCATAAGCA
  TCGAAGGTACCCAAATGGGGCCCATGCCCACTGCTGGAAATAACTCGCTAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016574 unedited
  NTGTTGGTGGCTNCTTCNGATTTTATTAGTTTGGTGCATGGCTAGTGATGTTACAGTAGT
  CTAAGGCCCCAGGGGTCTCTCCCAAGCTGCAGCTTCCCCTCCTTCCTCAGGCAGGGCCAG
  GCCAGGCCAGATGGTTTTCAGCAGCCTCTTAGCCGTGGGCTCCTCTAAGGCAGAGGCAGT
  GGAAGGGAAGGGAAACAGGAGAGAGGAAAGGCCAGAAGGAATAGAAAAGGAAAGTGGTTT
  TGCGTCAGAGTGTGGCAGAGGCAGTCCCTCAAAGAACCTCTGATGTCCCCTAGCCCAGGC
  CCAGATAGAGTTCCAGGGCCTGACCTCCCTGCCAGGCCCCAGGCTGGCCAGCATGTGCTG
  TGAGAAGGGATACATTGCAGGGCCGTCAGAAGGCAGAAGGGTGGGCTAGGCCAAGGAGTT
  CCTCTCCCCATAGACCCTCCTGGGCCCTTCAAGATGAGGGCTCCTCTTCTCAGAGCATGT
  GGAAACCACTTGGGGAGCTGTAACGGGGTGAGGTGGGTCCGGACTAGCCTGGAAAGTAGA
  GGTCCACATCGGGGTGCGGGAGGGAGCTAAGGTTTTTGGCTTGGGAATCTCTGGGGTCCA
  ACCTGCAGTCTGGTATTTACATGGGTCTCCCANGACTGAAGTTGCCTGGCTCCTCGGCAA
  GAGAGCTTGCTTGCCTCCTGTGGGCCTTGCAAGGTGTGAACTGTCCATCTCTTCCCACCG
  CCTGCTTCAAGGCCAGGCCCACAAACAGAAAACTCAGCCTCTTGGGCCCTGACTCCAGCT
  CCAGCAACCCCTAGAGCCCCCAAAGGAAGGTCAAGGAAAGGCGGCTTGCTCTTTGGTGCC
  CAGGGATAGGGGGACTGGAGGTGGGAAGGGGGGACTTTATAAGCTGCCCCTGGACTGGGG
  GGGCCCAGCCCCAGGGCCTGC
  
• Restriction Sites: Please inquire
• ACCN: NM_016574
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016574.2, NP_057658.2
• RefSeq Size: 2556 bp
• RefSeq ORF: 1245 bp
• Locus ID: 1813
• UniProt ID: P14416
• Cytogenetics: 11q23.2
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Gap junction, Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an internal exon, as compared to variant 1, but maintains the same reading frame. It encodes an isoform (short), which is 29 aa shorter than isoform long.