PLA2G12A (BC057844) Human Untagged Clone

CAT#: SC126365

(untagged)-Human cDNA clone MGC:71846 IMAGE:5313492, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLA2G12A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC057844 edited
  AGCGAGGCCCTGCCTGCCAGCCGGGAAGGAGGCGTGGTTATGGAGCTGGCGGCTGCAACC
  CCGGGGCCCGCGCCGCTGCCTAGCGCTTCCCGGGGGCTCTTTGGGGACGCGCCCCCCGCC
  GCGGCTCGGGGACCCGTAGAGCCCGGCGCTGCGCGGTTGGCCCTTCTCCCGCGCCCCGCG
  CTCACCCTCCTGCTCCTCCTCATGGCAGCTGTTATCAGGTGCCTGGAGCAGGCCTAGACC
  ACCGACTGGAGGGCCACCCTGAAGACCATCCGGAACGGCGTTCATAAGATAGACACGTAC
  CTGAACGCCGCCTTGGACCTCCTGGGAGGCGAGGACGGTCTCTGCCAGTATAAATGCAGT
  GACGGATCTAAGCCTTTCCCACGTTATGGTTATAAACCCTCCCCACCGAATGGATGTGGC
  TCTCCACTGTTTGGCGTTCATCTTAACATTGGTATCCCTTCCCTGACAAAGTGTTGCAAC
  TAACACGACAGGTGCTATGAGAACTGTGGCAAAAGCAAGAATGACTGTGATGAGAAATTC
  CAGTATTGCCTCTCCAAGATCTGCTGAGAAGTACAGAAAACACTAGGACTAACTTAGCAT
  GTTCAGGCATGTGAAACAACAATGGAGCTCTTGTTTGACAGTGTTATACATTTAGGTTGT
  AAACCATATCTGGACAGCCAACGAGCCACATGCAGGTGTCATTATGAAGAAAAAACCGAT
  ATTTAAAGGAGATGCTGACAGCTGGTGACAGATGAAGACGGAAGAACATAACCTTTGACA
  AATAACTAATGTTTTTACAACATAAAACTATCTGATTTTTGTGAAAAAAGAAAAACAAAC
  ATAGTTGGAGCTACCTGTACATCTCCCATATTGCAACACCTCCCTTTCCCAAAGATGTAC
  CCATTATCCTAAATTTAGTGTTTATTATTCCTGAGCATTTCTATTTCTGTGTAGGTATCC
  TTAAGCAATACATAGTATTTTTTTTGCCAGCTTTTAAAAATTATATCATTGGTTACATAC
  AGTGTAATTCTTCAACCTTTTTCTACTCAACCCTGTTTGTAAGCTTCACTCAAATTGACA
  TGTATACTGTATCTAGTTAGCTGATTTTCATTTCTGTTATGATTCCATAGCATGAAAATA
  AGTCACTTTCCCAATGGCTTACCATTATAGAGTGCTATGAACTTTTTGTATATGACTCTT
  GGTGTACGTATGTGAATTTCTCTAGAGCATATACTTAGGAATGGAATTGCTGGCTGCATT
  AGTCAGGTTTCTCTAGAGGAACAGAACTAATAGGATATATATATATATATATGTGTGTGT
  GTGTGTGTATATATATATGTGTGTGTGTGTGTATATATATGTGTGTATATATATATGTGT
  ATATATATATATAGTCCCACAATAGGCTGTCTGCATGCTCTATTAACCATCACACTGGCT
  GATATGGTTTGGCTCTGTGTTCCCACCCAAATCTCATCTTGAATAGTAATCCCATAATCC
  CCATGTGTCCTGGGAGGCACCTTGTGGGAGGTAATTGAATCATGGTTTCCTTCATGCTGT
  TCTTGTGATAGGAGTGAGTTCTCACGAGATCTGATGGTTTTATAAGCATCTGGCATTTCC
  CCTGCTGGCACTCATTCTCTCTCCTGCTGCCCTGTGAAGAGGTGCCTTTCACCATGATTT
  TAAGTTTCCTGAGGGCTCCCCAGCCATGTGGAACTGTGAGTCAATTAAACCTCTTTTCTT
  TATAAACAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC057844 unedited
  GTCATAATTTGTATACGACTCACTATAGGCGGCCGCATAACTTCGTATAGCATACATTAT
  ACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGAGCGAGGC
  CCTGCCTGCCAGCCGGGAAGGAGGCGTGGTTATGGAGCTGGCGGCTGCAACCCCGGGGCC
  CGCGCCGCTGCCTAGCGCTTCCCGGGGGCTCTTTGGGGACGCGCCCCCCGCCGCGGCTCG
  GGGACCCGTAGAGCCCGGCGCTGCGCGGTTGGCCCTTCTCCCGCGCCCCGCGCTCACCCT
  CCTGCTCCTCCTCATGGCAGCTGTTATCAGGTGCCTGGAGCAGGCCTAGACCACCGACTG
  GAGGGCCACCCTGAAGACCATCCGGAACGGCGTTCATAAGATAGACACGTACCTGAACGC
  CGCCTTGGACCTCCTGGGAGGCGAGGACGGTCTCTGCCAGTATAAATGCAGTGACGGATC
  TAAGCCTTTCCCACGTTATGGTTATAAACCCTCCCCACCGAATGGATGTGGCTCTCCACT
  GTTTGGCGTTCATCTTAACATTGGTATCCCTTCCCCTGACAAGTGTTGCAACTAACACGA
  CAGGTGCTATGAGAACTGTGGCAAAAGCAAGAATGACTGTGATGAGAAATTCCAGTATTG
  CCTCCTCCAGATCTGCTGAGAAGTACAGAAAACACTAGGACTAACTTTANCATGTTCAGG
  CATGTGAAACCAACATGGGAAGCTCTTGGTTGACAGTGTTATACATTTAAGTTGGTAAAC
  CATATCTGGGACAGCAACGAGCCACAGGCAGGTGTCCATTTTGAAGAAAAAACCGATATT
  AAAAGGAAATGCTGACAGCTGGTGACAGATGAAGACCGGAGAACATAACCCTTGACAAAT
  AACAAAGTTTTTACACATAAAACTTTTGATTTTTGTTGAAAAAGAAA
  
• Restriction Sites: Please inquire
• ACCN: BC057844
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057844.1, AAH57844.1
• RefSeq Size: 1762 bp
• Locus ID: 81579
• Cytogenetics: 4q25
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway
• Gene Summary: Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s (Gelb et al., 2000 [PubMed 11031251]).[supplied by OMIM, Mar 2008]