GLI1 (NM_005269) Human Untagged Clone

CAT#: SC125780

GLI1 (untagged)-Human GLI family zinc finger 1 (GLI1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GLI1
• Synonyms: GLI; PAPA8; PPD1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005269 edited
  CCCAGACAGAGTGTCCCCACACCCTCCTCTGAGACGCCATGTTCAACTCGATGACCCCAC
  CACCAATCAGTAGCTATGGCGAGCCCTGCTGTCTCCGGCCCCTCCCCAGTCAGGGGGCCC
  CCAGTGTGGGGACAGAAGGACTGTCTGGCCCGCCCTTCTGCCACCAAGCTAACCTCATGT
  CCGGCCCCCACAGTTATGGGCCAGCCAGAGAGACCAACAGCTGCACCGAGGGCCCACTCT
  TTTCTTCTCCCCGGAGTGCAGTCAAGTTGACCAAGAAGCGGGCACTGTCCATCTCACCTC
  TGTCGGATGCCAGCCTGGACCTGCAGACGGTTATCCGCACCTCACCCAGCTCCCTCGTAG
  CTTTCATCAACTCGCGATGCACATCTCCAGGAGGCTCCTACGGTCATCTCTCCATTGGCA
  CCATGAGCCCATCTCTGGGATTCCCAGCCCAGATGAATCACCAAAAAGGGCCCTCGCCTT
  CCTTTGGGGTCCAGCCTTGTGGTCCCCATGACTCTGCCCGGGGTGGGATGATCCCACATC
  CTCAGTCCCGGGGACCCTTCCCAACTTGCCAGCTGAAGTCTGAGCTGGACATGCTGGTTG
  GCAAGTGCCGGGAGGAACCCTTGGAAGGTGATATGTCCAGCCCCAACTCCACAGGCATAC
  AGGATCCCCTGTTGGGGATGCTGGATGGGCGGGAGGACCTCGAGAGAGAGGAGAAGCGTG
  AGCCTGAATCTGTGTATGAAACTGACTGCCGTTGGGATGGCTGCAGCCAGGAATTTGACT
  CCCAAGAGCAGCTGGTGCACCACATCAACAGCGAGCACATCCACGGGGAGCGGAAGGAGT
  TCGTGTGCCACTGGGGGGGCTGCTCCAGGGAGCTGAGGCCCTTCAAAGCCCAGTACATGC
  TGGTGGTTCACATGCGCAGACACACTGGCGAGAAGCCACACAAGTGCACGTTTGAAGGGT
  GCCGGAAGTCATACTCACGCCTCGAAAACCTGAAGACGCACCTGCGGTCACACACGGGTG
  AGAAGCCATACATGTGTGAGCACGAGGGCTGCAGTAAAGCCTTCAGCAATGCCAGTGACC
  GAGCCAAGCACCAGAATCGGACCCATTCCAATGAGAAGCCGTATGTATGTAAGCTCCCTG
  GCTGCACCAAACGCTATACAGATCCTAGCTCGCTGCGAAAACATGTCAAGACAGTGCATG
  GTCCTGACGCCCATGTGACCAAACGGCACCGTGGGGATGGCCCCCTGCCTCGGGCACCAT
  CCATTTCTACAGTGGAGCCCAAGAGGGAGCGGGAAGGAGGTCCCATCAGGGAGGAAAGCA
  GACTGACTGTGCCAGAGGGTGCCATGAAGCCACAGCCAAGCCCTGGGGCCCAGTCATCCT
  GCAGCAGTGACCACTCCCCGGCAGGGAGTGCAGCCAATACAGACAGTGGTGTGGAAATGA
  CTGGCAATGCAGGGGGCAGCACTGAAGACCTCTCCAGCTTGGACGAGGGACCTTGCATTG
  CTGGCACTGGTCTGTCCACTCTTCGCCGCCTTGAGAACCTCAGGCTGGACCAGCTACATC
  AACTCCGGCCAATAGGGACCCGGGGTCTCAAACTGCCCAGCTTGTCCCACACCGGTACCA
  CTGTGTCCCGCCGCGTGGGCCCCCCAGTCTCTCTTGAACGCCGCAGCAGCAGCTCCAGCA
  GCATCAGCTCTGCCTATACTGTCAGCCGCCGCTCCTCCCTGGCCTCTCCTTTCCCCCCTG
  GCTCCCCACCAGAGAATGGAGCATCCTCCCTGCCTGGCCTTATGCCTGCCCAGCACTACC
  TGCTTCGGGCAAGATATGCTTCAGCCAGAGGGGGTGGTACTTCGCCCACTGCAGCATCCA
  GCCTGGATCGGATAGGTGGTCTTCCCATGCCTCCTTGGAGAAGCCGAGCCGAGTATCCAG
  GATACAACCCCAATGCAGGGGTCACCCGGAGGGCCAGTGACCCAGCCCAGGCTGCTGACC
  GTCCTGCTCCAGCTAGAGTCCAGAGGTTCAAGAGCCTGGGCTGTGTCCATACCCCACCCA
  CTGTGGCAGGGGGAGGACAGAACTTTGATCCTTACCTCCCAACCTCTGTCTACTCACCAC
  AGCCCCCCAGCATCACTGAGAATGCTGCCATGGATGCTAGAGGGCTACAGGAAGAGCCAG
  AAGTTGGGACCTCCATGGTGGGCAGTGGTCTGAACCCCTATATGGACTTCCCACCTACTG
  ATACTCTGGGATATGGGGGACCTGAAGGGGCAGCAGCTGAGCCTTATGGAGCGAGGGGTC
  CAGGCTCTCTGCCTCTTGGGCCTGGTCCACCCACCAACTATGGCCCCAACCCCTGTCCCC
  AGCAGGCCTCATATCCTGACCCCACCCAAGAAACATGGGGTGAGTTCCCTTCCCACTCTG
  GGCTGTACCCAGGCCCCAAGGCTCTAGGTGGAACCTACAGCCAGTGTCCTCGACTTGAAC
  ATTATGGACAAGTGCAAGTCAAGCCAGAACAGGGGTGCCCAGTGGGGTCTGACTCCACAG
  GACTGGCACCCTGCCTCAATGCCCACCCCAGTGAGGGGCCCCCACATCCACAGCCTCTCT
  TTTCCCATTACCCCCAGCCCTCTCCTCCCCAATATCTCCAGTCAGGCCCCTATACCCAGC
  CACCCCCTGATTATCTTCCTTCAGAACCCAGGCCTTGCCTGGACTTTGATTCCCCCACCC
  ATTCCACAGGGCAGCTCAAGGCTCAGCTTGTGTGTAATTATGTTCAATCTCAACAGGAGC
  TACTGTGGGAGGGTGGGGGCAGGGAAGATGCCCCCGCCCAGGAACCTTCCTACCAGAGTC
  CCAAGTTTCTGGGGGGTTCCCAGGTTAGCCCAAGCCGTGCTAAAGCTCCAGTGAACACAT
  ATGGACCTGGCTTTGGACCCAACTTGCCCAATCACAAGTCAGGTTCCTATCCCACCCCTT
  CACCATGCCATGAAAATTTTGTAGTGGGGGCAAATAGGGCTTCACATAGGGCAGCAGCAC
  CACCTCGACTTCTGCCCCCATTGCCCACTTGCTATGGGCCTCTCAAAGTGGGAGGCACAA
  ACCCCAGCTGTGGTCATCCTGAGGTGGGCAGGCTAGGAGGGGGTCCTGCCTTGTACCCTC
  CTCCCGAAGGACAGGTATGTAACCCCCTGGACTCTCTTGATCTTGACAACACTCAGCTGG
  ACTTTGTGGCTATTCTGGATGAGCCCCAGGGGCTGAGTCCTCCTCCTTCCCATGATCAGC
  GGGGCAGCTCTGGACATACCCCACCTCCCTCTGGGCCCCCCAACATGGCTGTGGGCAACA
  TGAGTGTCTTACTGAGATCCCTACCTGGGGAAACAGAATTCCTCAACTCTAGTGCCTAAA
  GAGTAGGGAATCTCATCCATCACAGATCGCATTTCCTAAGGGGTTTCTATCCTTCCAGAA
  AAATTGGGGGAGCTGCAGTCCCCTGCACAAGATGCCCCAGGGATGGGAGGTATGGGCTGG
  GGGCTATGTATAGTCTGTATACGTTTTGAGGAGAAATTTGATAATGACACTGTTTCCTGA
  TAATAAAGGAACTGCATCAGAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005269 unedited
  NNGGGTTCAAAATTGTATACGACTCATATAGGCGGACCGCGAATTCGCACGAGGCCCAGA
  CAGAGTGTCCCCACACCCTCCTCTGAGACGCCATGTTCAACTCGATGACCCCACCACCAA
  TCAGTAGCTATGGCGAGCCCTGCTGTCTCCGGCCCCTCCCCAGTCAGGGGGCCCCCAGTG
  TGGGGACAGAAGGACTGTCTGGCCCGCCCTTCTGCCACCAAGCTAACCTCATGTCCGGCC
  CCCACAGTTATGGGCCAGCCAGAGAGACCAACAGCTGCACCGAGGGCCCACTCTTTTCTT
  CTCCCCGGAGTGCAGTCAAGTTGACCAAGAAGCGGGCACTGTCCATCTCACCTCTGTCGG
  ATGCCAGCCTGGACCTGCAGACGGTTATCCGCACCTCACCCAGCTCCCTCGTAGCTTTCA
  TCAACTCGCGATGCACATCTCCAGGAGGCTCCTACGGTCATCTCTCCATTGGCACCATGA
  GCCCATCTCTGGGATTCCCAGCCCAGATGAATCACCAAAAAGGGCCCTCGCCTTCCTTTG
  GGGTCCAGCCTTGTGGTCCCCATGACTCTGCCCGGGGTGGGATGATCCCACATCCTCAGT
  CCCGGGGACCCTTCCCAACTTGCCAGCTGAAGTCTGAGCTGGACATGCTGGTTGGCAAGT
  GCCGGGAGGAACCCTTGGAAGGTGATATGTCCAGCCCCAACTCCACAGGCATACAGGATC
  CCCTGTTGNGGATGCTGGATGGGCGGGAGGACCTCGAGAGAGAGGAAAGCGTGAGCCTGA
  ATCTGTGTATGAAACTGACTGCCGTTGGGATGGCTGCAGCCAGGAATTTGACTCCCAAGA
  CAGCTGGTGCACCACATCAACAGCGAGCACATCCACGGNGAGCGGAAGGAGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005269
• Insert Size: 3600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005269.1, NP_005260.1
• RefSeq Size: 3600 bp
• RefSeq ORF: 3321 bp
• Locus ID: 2735
• UniProt ID: P08151
• Cytogenetics: 12q13.3
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer
• Gene Summary: This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
  Transcript Variant: This variant (1) encodes the full length protein (isoform 1).