hCG (CGA) (NM_000735) Human Untagged Clone

CAT#: SC119734

CGA (untagged)-Human glycoprotein hormones, alpha polypeptide (CGA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: hCG
• Synonyms: CG-ALPHA; FSHA; GPA1; GPHa; GPHA1; HCG; LHA; TSHA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119734 sequence for NM_000735 edited (data generated by NextGen Sequencing)
  ATGGATTACTACAGAAAATATGCAGCTATCTTTCTGGTCACATTGTCGGTGTTTCTGCAT
  GTTCTCCATTCCGCTCCTGATGTGCAGGATTGCCCAGAATGCACGCTACAGGAAAACCCA
  TTCTTCTCCCAGCCGGGTGCCCCAATACTTCAGTGCATGGGCTGCTGCTTCTCTAGAGCA
  TATCCCACTCCACTAAGGTCCAAGAAGACGATGTTGGTCCAAAAGAACGTCACCTCAGAG
  TCCACTTGCTGTGTAGCTAAATCATATAACAGGGTCACAGTAATGGGGGGTTTCAAAGTG
  GAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAA
  
  Clone variation with respect to NM_000735.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000735 unedited
  GCTGGATTTAGGNAATCCGNATTTCACTATAGGGCGAGCCGCGCAATTCGGCACCAGNAC
  GAAGCTAAAATCCCTCTTCGGATCCACAGTCAACCGCCCTGAACACTCCTGCAAAAAGCC
  CAGAGAAAGGAGCGCCATGGATTACTACAGAAAATATGCAGCTATCTTTCTGGTCACATT
  GTCGGTGTTTCTGCATGTTCTCCATTCCGCTCCTGTATGTTCAGGATTGCCCAGAATGCA
  CGCTACAGGAAAACCCATTCTTCTCCCAGCCGGGTGCCCCAATACTTCAGTGCATGGGCT
  GCTGCTTCTCTAGAGCATATCCCACTCCACTAAGGTCCAAGAAGACGATGTTGGTCCAAA
  AGAACGTCACCTCAGAGTCCACTTGCTGTGTAGCTAAATCATATAACAGGGTCACAGTAA
  TGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACA
  AATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAAT
  TAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCAC
  TTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACA
  ATCAGCAGTCTAGTTCTTTTCATTTGGGATGAATACAGCATTTAGCTTGGTCCACTGCAA
  ATAAAGCCTTTTTAATCATCAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCNGCCGCG
  GTCATAGCTGTTTCCCTGACAGATCCCGGGTGGCATCCCTGTGACCCTCCCCAATGCCTT
  TCTGGGCCTGGAAGTGCCCCTCCAGTGCCAACAAACTTGGGCCTATAAATAAAGTGCATC
  ATTTTGNCTGACAAGGTGTCCCTCTTAAATTATGGGTTGAGAGGGTTGTGTTGAAAACCA
  GGGCAAATTTGGAAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000735
• Insert Size: 700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000735.2, NP_000726.1
• RefSeq Size: 704 bp
• RefSeq ORF: 351 bp
• Locus ID: 1081
• UniProt ID: P01215
• Cytogenetics: 6q14.3
• Domains: GHA
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Autoimmune thyroid disease, GnRH signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.