Aconitase 2 (ACO2) (NM_001098) Human Untagged Clone

CAT#: SC119428

ACO2 (untagged)-Human aconitase 2, mitochondrial (ACO2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Aconitase 2
• Synonyms: ACONM; HEL-S-284; ICRD; OCA8; OPA9
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001098, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGCCCTACAGCCTACTGGTGACTCGGCTGCAGAAAGCTCTGGGTGTGCGGCAGTACCATGTGGCCT
  CAGTCCTGTGCCAACGGGCCAAGGTGGCGATGAGCCACTTTGAGCCCAACGAGTACATCCATTATGACCT
  GCTAGAGAAGAACATTAACATTGTTCGCAAACGACTGAACCGGCCGCTGACACTCTCGGAGAAGATTGTG
  TATGGACACCTGGATGACCCCGCCAGCCAGGAAATTGAGCGAGGCAAGTCGTACCTGCGGCTGCGGCCGG
  ACCGTGTGGCCATGCAGGATGCGACGGCCCAGATGGCCATGCTCCAGTTCATCAGCAGCGGGCTGTCCAA
  GGTGGCTGTGCCATCCACCATCCACTGTGACCATCTGATTGAAGCCCAGGTTGGGGGCGAGAAAGACCTG
  CGCCGGGCCAAGGACATCAACCAGGAAGTTTATAATTTCCTGGCAACTGCAGGTGCCAAATATGGCGTGG
  GCTTCTGGAAGCCTGGATCTGGAATCATTCACCAGATTATTCTGGAAAACTATGCGTACCCTGGTGTTCT
  TCTGATTGGCACTGACTCCCACACCCCCAATGGTGGCGGCCTTGGGGGCATCTGCATTGGAGTTGGGGGT
  GCCGATGCTGTGGATGTCATGGCTGGGATCCCCTGGGAGCTGAAGTGCCCCAAGGTGATTGGCGTGAAGC
  TGACGGGCTCTCTCTCCGGTTGGTCCTCACCCAAAGATGTGATCCTGAAGGTGGCAGGCATCCTCACGGT
  GAAAGGTGGCACAGGTGCAATCGTGGAATACCACGGGCCTGGTGTAGACTCCATCTCCTGCACTGGCATG
  GCGACAATCTGCAACATGGGTGCAGAAATTGGGGCCACCACTTCCGTGTTCCCTTACAACCACAGGATGA
  AGAAGTACCTGAGCAAGACCGGCCGGGAAGACATTGCCAATCTAGCTGATGAATTCAAGGATCACTTGGT
  GCCTGACCCTGGCTGCCATTATGACCAACTAATTGAAATTAACCTCAGTGAGCTGAAGCCACACATCAAT
  GGGCCCTTCACCCCTGACCTGGCTCACCCTGTGGCAGAAGTGGGCAAGGTGGCAGAGAAGGAAGGATGGC
  CTCTGGACATCCGAGTGGGTCTAATTGGTAGCTGCACCAATTCAAGCTATGAAGATATGGGGCGCTCAGC
  AGCTGTGGCCAAGCAGGCACTGGCCCATGGCCTCAAGTGCAAGTCCCAGTTCACCATCACTCCAGGTTCC
  GAGCAGATCCGCGCCACCATTGAGCGGGACGGCTATGCACAGATCTTGAGGGATCTGGGTGGCATTGTCC
  TGGCCAATGCTTGTGGCCCCTGCATTGGCCAGTGGGACAGGAAGGACATCAAGAAGGGGGAGAAGAACAC
  AATCGTCACCTCCTACAACAGGAACTTCACGGGCCGCAACGACGCAAACCCCGAGACCCATGCCTTTGTC
  ACGTCCCCAGAGATTGTCACAGCCCTGGCCATTGCGGGAACCCTCAAGTTCAACCCAGAGACCGACTACC
  TGACGGGCACGGATGGCAAGAAGTTCAGGCTGGAGGCTCCGGATGCAGATGAGCTTCCCAAAGGGGAGTT
  TGACCCAGGGCAGGACACCTACCAGCACCCACCCAAGGACAGCAGCGGGCAGCATGTGGACGTGAGCCCC
  ACCAGCCAGCGCCTGCAGCTCCTGGAGCCTTTTGACAAGTGGGATGGCAAGGACCTGGAGGACCTGCAGA
  TCCTCATCAAGGTCAAAGGGAAGTGTACCACTGACCACATCTCAGCTGCTGGCCCCTGGCTCAAGTTCCG
  TGGGCACTTGGATAACATCTCCAACAACCTGCTCATTGGTGCCATCAACATTGAAAACGGCAAGGCCAAC
  TCCGTGCGCAATGCCGTCACTCAGGAGTTTGGCCCCGTCCCTGACACTGCCCGCTACTACAAGAAACATG
  GCATCAGGTGGGTGGTGATCGGAGACGAGAACTACGGCGAGGGCTCGAGCCGGGAGCATGCAGCTCTGGA
  GCCTCGCCACCTTGGGGGCCGGGCCATCATCACCAAGAGCTTTGCCAGGATCCACGAGACCAACCTGAAG
  AAACAGGGCCTGCTGCCTCTGACCTTCGCTGACCCGGCTGACTACAACAAGATTCACCCTGTGGACAAGC
  TGACCATTCAGGGCCTGAAGGACTTCACCCCTGGCAAGCCCCTGAAGTGCATCATCAAGCACCCCAACGG
  GACCCAGGAGACCATCCTCCTGAACCACACCTTCAACGAGACGCAGATTGAGTGGTTCCGCGCTGGCAGT
  GCCCTCAACAGAATGAAGGAACTGCAACAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001098 unedited
  ACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGCACAAAAGGCGCCCTACAGCCTA
  CTGGTGACTCGGCTGCAGAAAGCTCTGGGTGTGCGGCAGTACCATGTGGCCTCAGTCCTG
  TGCCAACGGGCCAAGGTGGCGATGAGCCACTTTGAGCCCAACGAGTACATCCATTATGAC
  CTGCTAGAGAAGAACATTAACATTGTTCGCAAACGACTGAACCGGCCGCTGACACTCTCG
  GAGAAGATTGTGTATGGACACCTGGATGACCCCGCCAGCCAGGAAATTGAGCGAGGCAAG
  TCGTACCTGCGGCTGCGGCCGGACCGTGTGGCCATGCAGGATGCGACGGCCCAGATGGCC
  ATGCTCCAGTTCATCAGCAGCGGGCTGTCCAAGGTGGCTGTGCCATCCACCATCCACTGT
  GACCATCTGATTGAAGCCCAGGTTGGGGGCGAGAAAGACCTGCGCCGGGCCAAGGACATC
  AACCAGGAAGTTTATAATTTCCTGGCAACTGCAGGTGCCAAATATGGCGTGGGCTTCTGG
  AAGCCTGGATCTGGAATCATTCACCAGATTATTCTGGAAAACTATGCGTACCCTGGTGTT
  CTTCTGATTGGCACTGACTCCCACACCCCCAATGGTGGCGGCCTTGGNGGCATCTGCATT
  GGAGTTGGGGGTGCCGATGCTGTGGATGTCATGGCTGGGATCCCCTGGGAGTTGAAGTGC
  CCCAGGGTGATTGGCGTGAAGCTGACGGGCTCTCTCTCCGGTTGGTCCTCACCAAAGATG
  TGATCCTGAAGGTGCANGCATNCTCACGGTGAAGGGTGCACAGGTGNCATCGTGGATACC
  ACGGCCCTGGTGTANACTCCATCTNCTGCACTGGCATGGCGACAATCTGCANCATGGGTG
  CAGAAAATGGGGCAACCACTTCGTGTTCCTTACACCCAGGAGNANAAGTACCTGACAGAC
  GGNCGGAGAACTTGCATTACTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001098 unedited
  GCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTG
  GAATCTTGGTTTCCTTGCAAAAACAAAAGGGTCTTTTAAATCCTTCAGCCCCCCATGAAA
  TGATCAGGCCGGAAAAAAACTTTAAATGGGAGTCTTGTCATCAAAAATAAATACCGTTGC
  CTGGAGCTGGTTAAAATACTGAACCCAACTCCAAATAGGAAAACGGGGGCTTAAAAGTTA
  TTTTAAAAACCCCCCCACCACAACCACAGTCACTCCGTGGGCTAAACGGGGAACAAGAAA
  CATGTCTGGGCACACCATCTTGGAATAAGAAACCCTGGCTGGACGGATCGGATCCACTGG
  TGGGCCACCTGGAGCTGAACTTTACGCCCCCGGCGGGGCGGGGAAGCACTGCCCTCACTG
  GTGCAGGTCCTTTATTTTGTTGAGGGCACTGGCAGCGCGGAACCACTCAATCTGCGTCTT
  GTTGAAAGGGTGGGTCAAGAGGATGGTCTCCTGGGTCCCCTTGGGGTGCTTGATGATGCA
  CTTTAAGGGCTTGCCAGGGGTGAAATCCCTTAAGCCCTGAATGGTCAACTTGTCCCAGGG
  TGAATTTTGTTGTAATCACCCGGGTCACCCAAAGTCATAAGCCACAAGCCCTGTTTCCTT
  ACGGTGGGCCTCGGGGATCCTCGCACAACTTTTGGTGAATGAATGCCCCGGCCCCCAAGG
  TGGCGAAGGGTTCCCAAACTGGTTGCTCCCCGGCTCCAAACCTCCGCCGCATTTTCTCGT
  TTCCGATTCCCCCCCCCTTAATCCCAGTCTCCTTTTCCCTTTCCCCCCGGTCTTGGCCCG
  CTCTCATCTTCCCCCGTACCCTCTCTCCCATTGTTTCCCCCTCCACCCTTTTCTTCCTCC
  CCCCCTTCACCTCTCTTCTTTCGTTCCTTCACCGCCCTCTCCTTCCCCCCCCCTTACCCC
  TCCTCCTACTTCTCCCCCTCCCCCATTTCTTCCATCTCCCCTCTACTCCCTCGCATCTTT
  CCTCTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001098
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098.2, NP_001089.1
• RefSeq Size: 2744 bp
• RefSeq ORF: 2343 bp
• Locus ID: 50
• UniProt ID: Q99798
• Cytogenetics: 22q13.2
• Domains: Aconitase_C, aconitase
• Protein Pathways: Citrate cycle (TCA cycle), Glyoxylate and dicarboxylate metabolism, Metabolic pathways
• Gene Summary: The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq, Jul 2008]