HLA-DPB1 (NM_002121) Human Untagged Clone

CAT#: SC118846

HLA (untagged)-Human major histocompatibility complex, class II, DP beta 1 (HLA-DPB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HLA-DPB1
• Synonyms: DPB1; HLA-DP; HLA-DP1B; HLA-DPB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118846 sequence for NM_002121 edited (data generated by NextGen Sequencing)
  ATGATGGTTCTGCAGGTTTCTGCGGCCCCCCGGACAGTGGCTCTGACGGCGTTACTGATG
  GTGCTGCTCACATCTGTGGTCCAGGGCAGGGCCACTCCAGAGAATTACCTTTTCCAGGGA
  CGGCAGGAATGCTACGCGTTTAATGGGACACAGCGCTTCCTGGAGAGATACATCTACAAC
  CGGGAGGAGTTCGCGCGCTTCGACAGCGACGTGGGGGAGTTCCGGGCGGTGACGGAGCTG
  GGGCGGCCTGCTGCGGAGTACTGGAACAGCCAGAAGGACATCCTGGAGGAGAAGCGGGCA
  GTGCCGGACAGGATGTGCAGACACAACTACGAGCTGGGCGGGCCCATGACCCTGCAGCGC
  CGAGTCCAGCCTAGGGTGAATGTTTCCCCCTCCAAGAAGGGGCCCTTGCAGCACCACAAC
  CTGCTTGTCTGCCACGTGACGGATTTCTACCCAGGCAGCATTCAAGTCCGATGGTTCCTG
  AATGGACAGGAGGAAACAGCTGGGGTCGTGTCCACCAACCTGATCCGTAATGGAGACTGG
  ACCTTCCAGATCCTGGTGATGCTGGAAATGACCCCCCAGCAGGGAGATGTCTACACCTGC
  CAAGTGGAGCACACCAGCCTGGATAGTCCTGTCACCGTGGAGTGGAAGGCACAGTCTGAT
  TCTGCCCGGAGTAAGACATTGACGGGAGCTGGGGGCTTCGTGCTGGGGCTCATCATCTGT
  GGAGTGGGCATCTTCATGCACAGGAGGAGCAAGAAAGTTCAACGAGGATCTGCATAA
  
  Clone variation with respect to NM_002121.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002121 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTTTCATTTTGCC
  ATCCTTTTCCAGCTCCATGATGGTTCTGCAGGTTTCTGCGGCCCCCCGGACAGTGGCTCT
  GACGGCGTTACTGATGGTGCTGCTCACATCTGTGGTCCAGGGCAGGGCCACTCCAGAGAA
  TTACCTTTTCCAGGGACGGCAGGAATGCTACGCGTTTAATGGGACACAGCGCTTCCTGGA
  GAGATACATCTACAACCGGGAGGAGTTCGCGCGCTTCGACAGCGACGTGGGGGAGTTCCG
  GGCGGTGACGGAGCTGGGGCGGCCTGCTGCGGAGTACTGGAACAGCCAGAAGGACATCCT
  GGAGGAGAAGCGGGCAGTGCCGGACAGGATGTGCAGACACAACTACGAGCTGGGCGGGCC
  CATGACCCTGCAGCGCCGAGTCCAGCCTAGGGTGAATGTTTCCCCCTCCAAGAAGGGGCC
  CTTGCAGCACCACAACCTGCTTGTCTGCCACGTGACGGATTTCTACCCAGGCAGCATTCA
  AGTCCGATGGTTCCTGAATGGACAGGAGGAAACAGCTGGGGTCGTGTCCACCAACCTGAT
  CCGTAATGGAGACTGGACCTTCCAGATCCTGGTGATGCTGGAAATGACCCCCCAGCAGGG
  AGATGTCTACACCTGCCAAGTGGAGCACACCAGCCTGGATAGTCCTGTCACCGTGGAGTG
  GAAGGCACAGTCTGATTCTGCCCGGNAGTAGACATTGACGGGAGCTGGGGGCTTCGTGCT
  GGGGCTCATCATCTGTGGAGTGGGCATCTTCATGCACAGGNAGGAGCAGAAAGNTTCACG
  AGGNATCTGCATAACAGGGNTCCTGAGCTCACTGAAAAGACTATTGTGCCTTTAGNAAAG
  CATTNGCTGTGTTNCGNTAGCATCTGGCTNCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002121 unedited
  CGGCCGCTTTTAGNATCGAGTTTTTTTTTTTTTTTTTTATGTTGGGTTTTGATTATTTGT
  GGTGCTAAAAAAAAGGAACATGGTTGGAGGCCCAGTGAGAGAAACAGTGCTTTGAATCAA
  AGAGCAGAATGATAGAAACTGACTTCAGAGCAACTTCTTGGCAGCAGTATCCAATTTGGA
  AGTTGAAGGTCTGTCCTGGAGCCAGATGCTAACGAAACACAGCAAATGCTTTTCCTAAGG
  CACAATAGTCTTTTCAGTGAGCTCAGGAACCCTGTTTATGCAGATCCTCGTTGAACTTTC
  TTGCTCCTCCTGTGCATGAAGATGCCCACTCCACAGATGATGAGCCCCAGCACGAAGCCC
  CCAGCTCCCGTCAATGTCTTACTCCGGGCAGAATCAGACTGTGCCTTCCACTCCACGGTG
  ACAGGACTATCCAGGCTGGTGTGCTCCACTTGGCAGGTGTAGACATCTCCCTGCTGGGGG
  GTCATTTCCAGCATCACCAGGATCTGGAAGGTCCAGTCTCCATTACGGATCAGGTTGGTG
  GACACGACCCCAGCTGTTTCCTCCTGTCCATTCAGGAACCATCGGACTTGAATGCTGCCT
  GGGTAGAAATCCGTCACGTGGCAGACAAGCAGGTTGTGGTGCTGCAAGGGCCCCTTCTTG
  GAGGGGGAAACATTCACCCTAGGCTGGACTCGGCGCTGCAGGGTCATGGGCCCGCCCAGC
  TCGTAGTTGTGTCTGCACATCCTGTCCGGCACTGCCCGCTTCTCCTCCAGNATGTCCTTN
  CTGGNCTGTTCAGTACTNCGCAGCAGGCCGCCCCAGCTTCGTCACCGNCCGGAACTCCNC
  CACGTCGCTGTCNGAGCGCGCGAACTCCTCCCGGNTGTAGATGTATCTCTTCAGAAAGCG
  CTGNNGTCCATTAAACGCGTAGCATTCCTGCCGTCCCTGGNAAANGTANTNCTCTGNAGT
  GGCCCTGCCCTGNACCCAGNAGTGAGCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002121
• Insert Size: 1050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002121.4, NP_002112.3
• RefSeq Size: 1501 bp
• RefSeq ORF: 777 bp
• Locus ID: 3115
• UniProt ID: P04440
• Cytogenetics: 6p21.32
• Domains: MHC_II_beta, ig, IGc1
• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis
• Gene Summary: HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq, Jul 2008]