HLA DMB (HLA-DMB) (NM_002118) Human Untagged Clone

CAT#: SC118844

HLA (untagged)-Human major histocompatibility complex, class II, DM beta (HLA-DMB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HLA DMB
• Synonyms: D6S221E; RING7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118844 sequence for NM_002118 edited (data generated by NextGen Sequencing)
  ATGATCACATTCCTGCCGCTGCTGCTGGGGCTCAGCCTGGGCTGCACAGGAGCAGGTGGC
  TTCGTGGCCCATGTGGAAAGCACCTGTCTGTTGGATGATGCTGGGACTCCAAAGGATTTC
  ACATACTGCATCTCCTTCAACAAGGATCTGCTGACCTGCTGGGATCCAGAGGAGAATAAG
  ATGGCCCCTTGCGAATTTGGGGTGCTGAATAGCTTGGCGAATGTCCTCTCACAGCACCTC
  AACCAAAAAGACACCCTGATGCAGCGCTTGCGCAATGGGCTTCAGAATTGTGCCACACAC
  ACCCAGCCCTTCTGGGGATCACTGACCAACAGGACACGGCCACCATCTGTGCAAGTAGCC
  AAAACCACTCCTTTTAACACGAGGGAGCCTGTGATGCTGGCCTGCTATGTGTGGGGCTTC
  TATCCAGCAGAAGTGACTATCACGTGGAGGAAGAACGGGAAGCTTGTCATGCCTCACAGC
  AGTGCGCACAAGACTGCCCAGCCCAATGGAGACTGGACATACCAGACCCTCTCCCATTTA
  GCCTTAACCCCCTCTTACGGGGACACTTACACCTGTGTGGTAGAGCACACTGGGGCTCCT
  GAGCCCATCCTTCGGGACTGGACACCTGGGCTGTCCCCCATGCAGACCCTGAAGGTTTCT
  GTGTCTGCAGTGACTCTGGGCCTGGGCCTCATCATCTTCTCTCTTGGTGTGATCAGCTGG
  CGGAGAGCTGGCCACTCTAGTTACACTCCTCTTCCTGGGTCCAATTATTCAGAAGGATGG
  CACATTTCCTAG
  
  Clone variation with respect to NM_002118.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002118 unedited
  GGTTTGCGNATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCCCACAT
  CACTCATTTGGGGGGTCAAGGGACCCGGGCAATATAGTATTCTGCTCAGTGTCTGGAGAT
  CATCTACCCAGGCTGGGGCTTCTGGGACAGGCGAGGACCCACGGACCCTGGAAGAGCTGG
  TCCAGGGGACTGAACTCCCGGCATCTTTACAGAGCAGAGCATGATCACATTCCTGCCGCT
  GCTGCTGGGGCTCAGCCTGGGCTGCACAGGAGCAGGTGGCTTCGTGGCCCATGTGGAAAG
  CACCTGTCTGTTGGATGATGCTGGGACTCCAAAGGATTTCACATACTGCATCTCCTTCAA
  CAAGGATCTGCTGACCTGCTGGGATCCAGAGGAGAATAAGATGGCCCCTTGCGAATTTGG
  GGTGCTGAATAGCTTGGCGAATGTCCTCTCACAGCACCTCAACCAAAAAGACACCCTGAT
  GCAGCGCTTGCGCAATGGGCTTCAGAATTGTGCCACACACACCCAGCCCTTCTGGGGATC
  ACTGACCAACAGGACACGGCCACCATCTGTGCAAGTAGCCAAAACCACTCCTTTTAACAC
  GAGGGAGCCTGTGATGCTGGCCTGCTATGTGTGGGGCTTCTATCCAGCAGAAGTGACTAT
  CACGTGGAGGAAGAACGGGAAGCTTGTCATGCCTCACAGCAGTGCGCACAAGACTGCCCA
  GCCCAATGGAGACTGGACATACCAGACCCTCTCCCATTTAGCCTTAACCCCCTCTTACCG
  NGACACTTACACCTGTGTGGTAGAGCACACTGGGGCTTCTGAGCCATCCTTCGGNACTGG
  ACACCTGNGCTGTCCCCATGCAGACCCTGAAGTTTCTGTGTCTGCAGTGACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002118 unedited
  CCCGCAATCNAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTCCCACCAAAGTTTATTTTTTTGAAACAATCACCAGTTGTTGTCCT
  TTATGGCACACTGAAACCCCCAGGAGGGTTTTTAACTCCCTTCCTAAAATTATATTCATC
  CCAAAAATATACCCTTGGACAATATTTTGGTTACAGCATAACCCCAGAAAGGGGGGCCCC
  CAAGTTGCTAATTTTTACATAGGGGAAACTGGGAAATTAAAAAAATTGGATGGAAAAACC
  TTAGGATCCAAAATATTGTCGGGGGGTTAAAAATGTTGAAAAGGCATGGAACCATCATTG
  AGTTGAAATCTCCTTCTCACTTGGAGGGAAATTTGCAAAATTCTCCCTTTAGGAAAGGCG
  CCATCCTTCTGAATAATGGGCCCCAGAAAAAGGAGGGTAACTAAAGGGGCCAGTTTTCCC
  CCAGTTGATCCCCCCAAAAAAAAAAATGATGAGGCCCACGCCCAAAGTCACTGAAAACAC
  AAAAACCTTAAGGGTCTGATTGGGGGACACCCCAAGGGTCCACCCCCGAGGGTGGGCTAA
  GGACCCCCAGGGGCCCTACCACACGGGGTAATTTCCCCCTAAAGGGGGCCAACGCTATAT
  GGAAAGGGCCCGTCTCCCCAATTCTATTGGGCTGGCCAACCTTCCCCCCCCTGTCGGCAG
  GTCCCCAACTTCGCCGTGTTTCTCCCCGCCATCCCCCTTTGTTTGAAAAACGCGACATGG
  CCCGCACCTTTCCGGCCCCTCAGCTAAAAGAGGGCTTTTTTTTTTCTACATGTGGTAAAA
  CCACGCGCGAAAGACACCCCAAGGCCGCGCCCCGCCATCACCTCCGTCCTTTCTCCACTC
  CATATACCAAAAATTCTTCCCGCTTCGTGTTTCTGTCACCTTCCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002118
• Insert Size: 1470 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002118.3, NP_002109.1
• RefSeq Size: 1358 bp
• RefSeq ORF: 792 bp
• Locus ID: 3109
• UniProt ID: P28068
• Cytogenetics: 6p21.32
• Domains: MHC_II_beta, ig, IGc1
• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis
• Gene Summary: HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]