MEK1 (MAP2K1) (NM_002755) Human Untagged Clone

CAT#: SC118424

MAP2K1 (untagged)-Human mitogen-activated protein kinase kinase 1 (MAP2K1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MEK1
• Synonyms: CFC3; MAPKK1; MEK1; MEL; MKK1; PRKMK1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002755 edited
  ATGCCCAAGAAGAAGCCGACGCCCATCCAGCTGAACCCGGCCCCCGACGGCTCTGCAGTT
  AACGGGACCAGCTCTGCGGAGACCAACTTGGAGGCCTTGCAGAAGAAGCTGGAGGAGCTA
  GAGCTTGATGAGCAGCAGCGAAAGCGCCTTGAGGCCTTTCTTACCCAGAAGCAGAAGGTG
  GGAGAACTGAAGGATGACGACTTTGAGAAGATCAGTGAGCTGGGGGCTGGCAATGGCGGT
  GTGGTGTTCAAGGTCTCCCACAAGTCTTCTGGCCTGGTCATGGCCAGAAAGCTAATTCAT
  CTGGAGATCAAACCCGCAATCCGGAACCAGATCATAAGGGAGCTGCAGGTTCTGCATGAG
  TGCAACTCTCCGTACATCGTGGGCTTCTATGGTGCGTTCTACAGCGATGGCGAGATCAGT
  ATCTGCATGGAGCACATGGATGGAGGTTCTCTGGATCAAGTCCTGAAGAAAGCTGGAAGA
  ATTCCTGAACAAATTTTAGGAAAAGTTAGCATTGCTGTAATAAAAGGCCTGACATATCTG
  AGGGAGAAGCACAAGATCATGCACAGAGATGTCAAGCCCTCCAACATCCTAGTCAACTCC
  CGTGGGGAGATCAAGCTCTGTGACTTTGGGGTCAGCGGGCAGCTCATCGACTCCATGGCC
  AACTCCTTCGTGGGCACAAGGTCCTACATGTCGCCAGAAAGACTCCAGGGGACTCATTAC
  TCTGTGCAGTCAGACATCTGGAGCATGGGACTGTCTCTGGTAGAGATGGCGGTTGGGAGG
  TATCCCATCCCTCCTCCAGATGCCAAGGAGCTGGAGCTGATGTTTGGGTGCCAGGTGGAA
  GGAGATGCGGCTGAGACCCCACCCAGGCCAAGGACCCCCGGGAGGCCCCTTAGCTCATAC
  GGAATGGACAGCCGACCTCCCATGGCAATTTTTGAGTTGTTGGATTACATAGTCAACGAG
  CCTCCTCCAAAACTGCCCAGTGGAGTGTTCAGTCTGGAATTTCAAGATTTTGTGAATAAA
  TGCTTAATAAAAAACCCCGCAGAGAGAGCAGATTTGAAGCAACTCATGGTTCATGCTTTT
  ATCAAGAGATCTGATGCTGAGGAAGTGGATTTTGCAGGTTGGCTCTGCTCCACCATCGGC
  CTTAACCAGCCCAGCACACCAACCCATGCTGCTGGCGTCTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002755 unedited
  TCCCGTGGCCTTGTCTATATAAGCAGTTCTCGTTTAGTGAACCGNCAGAATTTCCGTTAT
  ACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTGAGAGAGAGAGAGGAAGGG
  AATCCCGGGCTGCCGAACCGCACGTTCAGCCCGCTCCGCTCCTGCAAGGCAGCCATTCGC
  CTCGCGTGCGCGATTCCGAGTCCTGGGCGGGTGGGGCGGGGGTCCACTGAGACCGCTACC
  GGCCCCTCGGCGCTGACGGGACCGCGCGGGGCGCACCCGCTGAAGGCAGCCCCGGGGCCC
  GCGGCCCGGACTTGGTCCTGCGCAGCGGGCGCGGGGCAGCGCAGCGGGAGGAAGCGAGAG
  GTGCTGCCCTCCCCCCGGAGTTGGAAGCGCGTTACCCGGGTCCAAAATGCCCAAGAAGAA
  GCCGACGCCCATCCAGCTGAACCCGGCCCCCGACGGCTCTGCAGTTAACGGGACCAGCTC
  TGCGGAGACCAACTTGGAGGCCTTGCAGAAAAAGCTGGAGGAGCTAGAGCTTGATGAGCA
  GCAGCGAAAGCGCCTTGAGGCCTTTCTTACCCAGAAGCAGAAAGTGGGAGAACTGAAGGA
  TGACGACTTTGAGAAGATCAGCGAGCTGGGGGCTGGCAATGGCGGCGTGGTGTTCAAGGT
  CTCCCACAAGTCTTCTGGCCTGGTCATGGCCAGAAAGCTAATTCATCTGGAGATCAAACC
  CGCATCCGGAACCGATCCATAAGGAACTGCAGGTTCTGCATGATTGCAACTCTCCCGACA
  TCGTGGGCTTCTATGGTGCGTTCTACAGCGATGGCGAGATCAGTATCTGCATGGAGCACA
  TGGATGGAAGGTTCCCTGGATCAAGTCCCGGAAGAAGCCGGCAAGAATTCCTGGACAATA
  TTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002755 unedited
  NGGAATTCTTGNACCGCGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTAGCTTTC
  ATCCTTTTTTTTTTATTTCATAGTATATTTATTAGAATAAGAGATTAGATTTGTTAAACA
  TCTAGGTTAAAATGGTTAAAAGGATTTTCATACAATTTTAGGCACTATACACGTTGTTTA
  CAACAGCATTGGTACTTGGATATGGGGAAAGATAAATCCGACATTTTAATATCTTGATCA
  ATTTGTGACATTCAAAATAATTCCATTTAAGAAACATTAATCAAAACTTAAAGAGACATA
  CCACTAAGTATCCCACACAGTATACTGAAAATAAATATAGAAATACAACCAGAAGTCTAC
  AGATCACCACAGTAGACAGACTGGTGAAGCCCCAGCTATCATGGCAGTGAAGGGCTCTGG
  CTAGATTTGGATGTCAACTGCTGAGTTCTACATGAAAAGCAAATAATAATAATAAAATAA
  CATGATGTACACTCTCTGATGCTCATTTTCATAGCAGCAAAGCATGCTTCACATGCACTG
  CCTGTGAAGGATCTCACAAGGCTCCCTCCTAGAGAGAAAGTATGCCCAGCAAGAATAAAG
  TACCACCAAATGTCAAAGAATCCCGAAAATACAGGCAGACAGCCAGTCATGGAGCAGGAA
  CAGCAGTGCAATTGTAAGAATGACTACCCACATTCACCAAAATTTCACTGTAATTCAGCC
  TGGCACAGGTTTTGATCATCAGCATACACACAAATAGCCCCAAGCACAAAGCCAATCCAC
  TTANGGGAACCCATAATAGTATAAGAATAAAGACCAGTATATTAAAAATGACAAGAGTNG
  AAATCTGGCACATGCTGNGTTCTTCATTCTTTGNNCACAGTGAAATGCACATTCTGACAG
  ANACAGGCATGGNAANNGAGCCCNANAGCGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002755
• Insert Size: 2550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002755.2, NP_002746.1
• RefSeq Size: 2222 bp
• RefSeq ORF: 1182 bp
• Locus ID: 5604
• UniProt ID: Q02750
• Cytogenetics: 15q22.31
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Oocyte meiosis, Pancreatic cancer, Pathways in cancer, Prion diseases, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Toll-like receptor signaling pathway, Vascular smooth muscle contraction, VEGF signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]