SDHC (NM_003001) Human Untagged Clone

CAT#: SC118274

SDHC (untagged)-Human succinate dehydrogenase complex, subunit C, integral membrane protein, 15kDa (SDHC), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SDHC
• Synonyms: CYB560; CYBL; PGL3; QPS1; SDH3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003001 edited
  ATGGCTGCGCTGTTGCTGAGACACGTTGGTCGTCATTGCCTCCGAGCCCACTTTAGCCCT
  CAGCTCTGTATCAGAAATGCTGTTCCTTTGGGAACCACGGCCAAAGAAGAGATGGAGCGG
  TTCTGGAATAAGAATATAGGTTCAAACCGTCCTCTGTCTCCCCACATTACTATCTACAGT
  TGGTCTCTTCCCATGGCGATGTCCATCTGCCACCGTGGCACTGGTATTGCTTTGAGTGCA
  GGGGTCTCTCTTTTTGGCATGTCGGCCCTGTTACTCCCTGGGAACTTTGAGTCTTATTTG
  GAACTTGTGAAGTCCCTGTGTCTGGGGCCAGCACTGATCCACACAGCTAAGTTTGCACTT
  GTCTTCCCTCTCATGTATCATACCTGGAATGGGATCCGACACTTGATGTGGGACCTAGGA
  AAAGGCCTGAAGATTCCCCAGCTATACCAGTCTGGAGTGGTTGTCCTGGTTCTTACTGTG
  TTGTCCTCTATGGGGCTGGCAGCCATGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003001 unedited
  CCGCGAATTCGGCACGAGCCGGAACCCAAGATGGCTGCGCTGTTGCTGAGACACGTTGGT
  CGTCATTGCCTCCGAGCCCACTTTAGCCCTCAGCTCTGTATCAGAAATGCTGTTCCTTTG
  GGAACCACGGCCAAAGAAGAGATGGAGCGGTTCTGGAATAAGAATATAGGTTCAAACCGT
  CCTCTGTCTCCCCACATTACTATCTACAGTTGGTCTCTTCCCATGGCGATGTCCATCTGC
  CACCGTGGCACTGGTATTGCTTTGAGTGCAGGGGTCTCTCTTTTTGGCATGTCGGCCCTG
  TTACTCCCTGGGAACTTTGAGTCTTATTTGGAACTTGTGAAGTCCCTGTGTCTGGGGCCA
  GCACTGATCCACACAGCTAAGTTTGCACTTGTCTTCCCTCTCATGTATCATACCTGGAAT
  GGGATCCGACACTTGATGTGGGACCTAGGAAAAGGCCTGAAGATTCCCCAGCTATACCAG
  TCTGGAGTGGTTGTCCTGGTTCTTACTGTGTTGTCCTCTATGGGGCTGGCAGCCATGTGA
  AGAAAGGAGGCTCCCAGCATCATCTTCCTACACATTATTACATTCACCCATCTTTCTGTT
  TGTCATTCTTATCTCCAGCCTGGGAAAAGTTCTCCTTATTTGGTTAGATCCTTTTGTATT
  TTCAGATCTCCTTGGAGCAGTAGAGTACCTGGTAGACCATAATAGTGGAAAAGGGTCTAG
  TTTTTCCCTTTGGTTCTAAGATGAGGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003001 unedited
  CCCCCATTCCTTGCCCGCGGCCGCATTCTACGAGCGAGTTTTTTTTTTTTTTTTTTTTTA
  AAATGAGTATTTAATTCAGTTTCAGAAAAAAGGGTACATTACTTCGATGAGAAGCATAAA
  ATTAAGAGGCTTTTCTGGAAATCCAAGAAAAAGAAATAACCATTAATATGTTCAATGATA
  ATTTTCTAGATCTTCCTCGTGTCCAAAGCTATATATTTTCTCCTCAAAAGCAGCTGCTCC
  AAGAACTAACTAGCCCTCTTTATCTCCCTCTAGCTGGGACAGACTAATTCCCCTCCACCC
  CCACCTCTCTTCTTTCAATAACACTGTGTAGGTGTTAATGGCCTGGGCTCTCACCAATGA
  AGAGAATTGTCCTTTAAGCCAGCAACCCACAGGCAGTGGCACCCAAAGCCTCACCAACCC
  ACCCCCCAACCCGGCCTATCCCCAGGGCAGAAGCCACAGAGCTGGCATTGTTTCCACTGT
  CTCAGGAAGAAGGAGCCAAAAGCTGACCTTTAGTACAAATTAAAGCCCAATATGGAGAGA
  GAATAACTGATTTTAGGCTTAGAGTAGGCAAGCTGCGGGCCAAAAAGGGGAGTTTTTGCT
  GCCTCTTCATTTTTTTAGACAGGGGGAAAACTAGACCCTTTTCCCTTATTACGGTCTACC
  AGGCCCTTTCCTGGTTTCAGGAGAACTGACAATCCACACGGATTTAACCAAAAAAGGCGA
  ACTTTTCCCACGCTGGAAAAATAATGACCACCCCACCACCGGTTAATGCTATACCGCGCC
  CCAAAATTATCCTTGGCAGCTTTACTTTTTTCCCCTGGTAGCCCACCCCCTCCGTGGTCT
  ACCATTCACCTACACGCAAACTCCTCACACCGGCTCGTTTGACAACATCCTGGCCTCCTC
  CCCCACCCCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003001
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003001.2, NP_002992.1
• RefSeq Size: 1315 bp
• RefSeq ORF: 510 bp
• Locus ID: 6391
• UniProt ID: Q99643
• Cytogenetics: 1q23.3
• Domains: Sdh_cyt
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Alzheimer's disease, Citrate cycle (TCA cycle), Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: This gene encodes one of four nuclear-encoded subunits that comprise succinate dehydrogenase, also known as mitochondrial complex II, a key enzyme complex of the tricarboxylic acid cycle and aerobic respiratory chains of mitochondria. The encoded protein is one of two integral membrane proteins that anchor other subunits of the complex, which form the catalytic core, to the inner mitochondrial membrane. There are several related pseudogenes for this gene on different chromosomes. Mutations in this gene have been associated with paragangliomas. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2013]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.