GFUS (NM_003313) Human Untagged Clone

CAT#: SC118067

TSTA3 (untagged)-Human tissue specific transplantation antigen P35B (TSTA3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GFUS
• Synonyms: FX; P35B; SDR4E1; TSTA3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118067 sequence for NM_003313 edited (data generated by NextGen Sequencing)
  ATGGGTGAACCCCAGGGATCCATGCGGATTCTAGTGACAGGGGGCTCTGGGCTGGTAGGC
  AAAGCCATCCAGAAGGTGGTAGCAGATGGAGCTGGACTTCCTGGAGAGGACTGGGTGTTT
  GTCTCCTCTAAAGACGCCGATCTCACGGATACAGCACAGACCCGCGCCCTGTTTGAGAAG
  GTCCAACCCACACACGTCATCCATCTTGCTGCAATGGTGGGGGGCCTGTTCCGGAATATC
  AAATACAATTTGGACTTCTGGAGGAAAAACGTGCACATGAACGACAACGTCCTGCACTCG
  GCCTTTGAGGTGGGCGCCCGCAAGGTGGTGTCCTGCCTGTCCACCTGTATCTTCCCTGAC
  AAGACGACCTACCCGATAGATGAGACCATGATCCACAATGGGCCTCCCCACAACAGCAAT
  TTTGGGTACTCGTATGCCAAGAGGATGATCGACGTGCAGAACAGGGCCTACTTCCAGCAG
  TACGGCTGCACCTTCACCGCTGTCATCCCCACCAACGTCTTCGGGCCCCACGACAACTTC
  AACATCGAGGATGGCCACGTGCTGCCTGGCCTCATCCACAAGGTGCACCTGGCCAAGAGC
  AGCGGCTCGGCCCTGACGGTGTGGGGTACAGGGAATCCGCGGAGGCAGTTCATATACTCG
  CTGGACCTGGCCCAGCTCTTTATCTGGGTCCTGCGGGAGTACAATGAAGTGGAGCCCATC
  ATCCTCTCCGTGGGCGAGGAAGATGAGGTCTCCATCAAGGAGGCAGCCGAGGCGGTGGTG
  GAGGCCATGGACTTCCATGGGGAAGTCACCTTTGATACAACCAAGTCGGATGGGCAGTTT
  AAGAAGACAGCCAGTAACAGCAAGCTGAGGACCTACCTGCCCGACTTCCGGTTCACACCC
  TTCAAGCAGGCGGTGAAGGAGACCTGTGCTTGGTTCACTGACAACTACGAGCAGGCCCGG
  AAGTGA
  
  Clone variation with respect to NM_003313.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003313 unedited
  ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGAGCGCACCCGGCCGGA
  AGCCGCTGTCGGGGAGCCGGCGGTGGGGCTGGACGCAGGTGCAACTGACATGGGTGAACC
  CCAGGGATCCATGCGGATTCTAGTGACAGGGGGCTCTGGGCTGGTAGGCAAAGCCATCCA
  GAAGGTGGTAGCAGATGGAGCTGGACTTCCTGGAGAGGACTGGGTGTTTGTCTCCTCTAA
  AGACGCCGATCTCACGGATACAGCACAGACCCGCGCCCTGTTTGAGAAGGTCCAACCCAC
  ACACGTCATCCATCTTGCTGCAATGGTGGGGGGCCTGTTCCGGAATATCAAATACAATTT
  GGACTTCTGGAGGAAAAACGTGCACATGAACGACAACGTCCTGCACTCGGCCTTTGAGGT
  GGGCGCCCGCAAGGTGGTGTCCTGCCTGTCCACCTGTATCTTCCCTGACAAGACGACCTA
  CCCGATAGATGAGACCATGATCCACAATGGGCCTCCCCACAACAGCAATTTTGGGTACTC
  GTATGCCAAGAGGATGATCGACGTGCAGAACAGGGCCTACTTCCAGCAGTACGGCTGCAC
  CTTCACCGCTGTCATCCCCACCAACGTCTTCGGGCCCCACGACAACTTCAACATCGNAGA
  TGGCCACGTGCTGCCTGGCCTCATCCACAAGGTGCACCTGGCCAAGAGCAGCGGCTCGGC
  CCTGACGGTNGTGGGTACAGGGGATCCGCGGAGGCAGTTCATATACTCGCTGGACCTGGC
  CCAGCTCTTTATCTGGNGTCCTGCCGGAGACAATGAAGTGGAGCCCATCATCCTCTNCGT
  GGGCGAAGAGATGAAGGTCTCCATCAAGGAGCANNCGANNGCGTGGTGGAGCCATGGACT
  TCATGGGAAGTCACCTTGATCCACAGTCGNAGGGCAGTTAGAAGAAGCCGTACAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003313 unedited
  GTACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCCTGTGAAAATGC
  ACTTTATTGGCTCCCAGGAGTGGGATGCAGGATCAGAGTGGACACGCGCAGGGGGCTGGT
  GTGGGGAGCAAAGCGCCGGGCCTGCCCGGGACCCTGGTTTCCCTGAGGACCAACGTGAAA
  TGGGGGCCCCCACTTGGAAAAAAAGCTTTGGGGCTTCAAAAACCGGTTGGAAATGGCAGG
  CCCCCACTTTCCTGGGGGGGGGGCCCCCTCCACTCTTTTCCACAGGGTTTAACAGGGGGG
  GCGGCCCCCCTCTTTTCCTCTTCCCCCCCACCCAGCGGGGGTTGCCGGGGACCCCCAACT
  ACTATTTTTTTTTCAAAAAAAAACTCACCTCGGCCCCCCTCCCCCTCTCATACCACGTCT
  TACAAACCCACACCCAACCACAACGTCTCTTCTCCTTTCCTCCCCTCGCCGTGGGGGGTG
  GGGGGGGTTGTTACAGAGTGTCCCGTCGCGCTTTTGTTTTATCTTCTCCCACTCCTCTTT
  TTCTAGCTTCGCTTCTTTTTTCACATACACTATCCCCTCCCTTTTACCTCCCCGTATCCC
  ATTCTCTCCTTTCCCTTTCCCCTACTATGTTCCGTCGCCTCCACCCCCCTTCCTTTCCAC
  TCTCCTTCCCTCTACTTCTACATCCACCTCCCTCCTCCCTCCTCCACCGCTCGCACCCCC
  CGCTCCCTCCCTTTCTTCTCCTTCTTTTCATTTATCACATTAAATCCCATACAAGTAATT
  AACATGTTTACCTACTTTAGCCATGCTTTCTTACAATACTCCTTCTTTCTTTCTCCCCAT
  CCTCCAAACCCCCCCAACCCACCTCTAATGTGAATAATAATCGCGCCACACGCGCATAAT
  TGTCAAGATGCGTCCCAAGCCAACACCCACGACTACCAAACAAACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003313
• Insert Size: 1430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003313.2, NP_003304.1
• RefSeq Size: 1312 bp
• RefSeq ORF: 966 bp
• Locus ID: 7264
• UniProt ID: Q13630
• Cytogenetics: 8q24.3
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways
• Gene Summary: Tissue specific transplantation antigen P35B is a NADP(H)-binding protein. It catalyze the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream translation start site compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1.