SUCLA2 (NM_003850) Human Untagged Clone

CAT#: SC117714

SUCLA2 (untagged)-Human succinate-CoA ligase, ADP-forming, beta subunit (SUCLA2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SUCLA2
• Synonyms: A-BETA; A-SCS; LINC00444; MTDPS5; SCS-betaA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117714 sequence for NM_003850 edited (data generated by NextGen Sequencing)
  ATGGCGGCCTCCATGTTCTACGGCAGGCTAGTGGCCGTGGCCACCCTTCGGAACCACCGG
  CCTCGGACGGCCCAGCGGGCTGCTGCTCAGGTTCTGGGAAGTTCTGGATTGTTTAATAAC
  CATGGACTCCAAGTACAGCAGCAACAGCAAAGGAATCTCTCACTACATGAATACATGAGT
  ATGGAATTATTGCAAGAAGCTGGTGTCTCCGTTCCCAAAGGATATGTGGCAAAGTCACCA
  GATGAAGCTTATGCAATTGCCAAAAAATTAGGTTCAAAAGATGTCGTGATAAAGGCACAG
  GTTTTAGCTGGTGGTAGAGGAAAAGGAACATTTGAAAGTGGCCTCAAAGGAGGAGTGAAG
  ATAGTTTTCTCTCCAGAAGAAGCAAAAGCTGTTTCTTCACAAATGATTGGGAAAAAATTG
  TTTACCAAGCAAACGGGAGAAAAGGGCAGAATATGCAATCAAGTATTGGTCTGTGAGCGA
  AAATATCCCAGGAGAGAATACTACTTTGCAATAACAATGGAAAGGTCATTTCAAGGTCCT
  GTATTAATAGGAAGTTCACATGGTGGTGTCAACATTGAAGATGTTGCTGCTGAGACTCCT
  GAAGCAATAATTAAAGAACCTATTGATATTGAAGAAGGCATCAAAAAGGAACAAGCTCTC
  CAGCTTGCACAGAAGATGGGATTTCCACCTAATATTGTGGAATCAGCAGCAGAAAACATG
  GTCAAGCTTTACAGCCTTTTTCTGAAATACGATGCAACCATGATAGAAATAAATCCAATG
  GTGGAAGATTCAGATGGAGCTGTATTGTGTATGGATGCAAAGATCAATTTTGACTCTAAT
  TCAGCCTATCGCCAAAAGAAAATCTTTGATCTACAGGACTGGACCCAGGAAGATGAAAGG
  GACAAAGATGCTGCTAAGGCAAATCTCAACTACATTGGCCTCGATGGAAATATAGGCTGC
  CTAGTAAATGGTGCTGGTTTGGCTATGGCCACAATGGATATAATAAAACTTCATGGAGGG
  ACTCCAGCCAACTTCCTTGATGTTGGTGGTGGTGCTACAGTCCATCAAGTAACAGAAGCA
  TTTAAGCTTATCACTTCAGATAAAAAGGTACTGGCTATTCTGGTCAACATTTTTGGAGGA
  ATCATGCGCTGTGATGTTATTGCACAGGGTATAGTCATGGCAGTAAAAGACTTGGAAATT
  AAAATACCTGTTGTGGTACGGTTACAAGGTACACGAGTCGATGATGCTAAGGCACTGATA
  GCGGACAGTGGACTTAAAATACTTGCTTGTGATGACTTGGATGAAGCTGCTAGAATGGTT
  GTAAAGCTCTCTGAAATAGTGACCTTAGCGAAGCAAGCACATGTGGATGTGAAATTTCAG
  TTGCCAATATGA
  
  Clone variation with respect to NM_003850.2
  595 t=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003850 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGGGTCCGACTCAGAA
  ATGGCGGCCTCCATGTTCTACGGCAGGCTAGTGGCCGTGGCCACCCTTCGGAACCACCGG
  CCTCGGACGGCCCAGCGGGCTGCTGCTCAGGTTCTGGGAAGTTCTGGATTGTTTAATAAC
  CATGGACTCCAAGTACAGCAGCAACAGCAAAGGAATCTCTCACTACATGAATACATGAGT
  ATGGAATTATTGCAAGAAGCTGGTGTCTCCGTTCCCAAAGGATATGTGGCAAAGTCACCA
  GATGAAGCTTATGCAATTGCCAAAAAATTAGGTTCAAAAGATGTCGTGATAAAGGCACAG
  GTTTTAGCTGGTGGTAGAGGAAAAGGAACATTTGAAAGTGGCCTCAAAGGAGGAGTGAAG
  ATAGTTTTCTCTCCAGAAGAAGCAAAAGCTGTTTCTTCACAAATGATTGGGAAAAAATTG
  TTTACCAAGCAAACGGGAGAAAAGGGCAGAATATGCAATCAAGTATTGGTCTGTGAGCGA
  AAATATCCCAGGAGAGAATACTACTTTGCAATAACAATGGAAAGGTCATTTCAAGGTCCT
  GTATTAATAGGAAGTTCACATGNGTGGTGTCAACATTGAAGATGTTGCTGCTGAGACTCC
  TGAAGCATAATTAAAGAACCTATTGATATTGAAGAAGGCATCAAAAAGGAACAAGCTCTC
  CAGCTTGCACAGAAGATGGGATTTCCACCTAATATTGTGGAATCAGCAGCAGAAACATGG
  TCAAGCTTTACAGNCCTTTNNTCTGAAATACGATGCACCATGATAGAAATAAATCCATGG
  TGGNAAGATCAGATGGAGCTGTATTGTGTATGGGATGCAAGATCAATTTTGACTCTAATT
  CAGCCTATCGCCAAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003850 unedited
  CAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCTATTGGGAAGATTTTTATTAACTTA
  AATTGACATTCTTAATTTTGTCTGTAAGTCCTTGGTATATATGCCTTTATTTGAAGCAAA
  CCTACAGGTGTTTCTTAATATGACAGAATCATGAAGACTTGCAGTTAATCAGTGTTTCCT
  AATGATTAAAACAATGTTCAAATAATTACAAAGTTACTTCATCAAAATACTTAGAAGAAT
  ATTCTGAGGAGTGTTTGAAAGCTCTGTTTATAAATAGTGATTGATACATTTATCATGTAT
  TTGGTGCTGAAGATAAACACTTTTTACATAAAACATTGTTTTAATATACTGCTCTACTAA
  TGAGGCTAGTTATTAGATATACTGTATTTTAACACTAAAGAATAAAGCTTTATCTTCGTA
  TTTATCTTATTTATAGGACTCTTATCAATGAAGAACTTTGTATCCAACAATAATAAACTG
  GCAAATTGCAAGTTACGTTTTTGTAGGAGAAGCAAAAAAGACTGGCTGCCACCAAAGAAA
  GAAATAACTGCCTATACATGCTTCGTCCAAACAGTCATTTCTGATGGACCATTAAATGCC
  ATCCAAATCCTTTTAAATGTTCGGGGCCTAAATGGCATTCCAATCCCCCCCCCTCAAAAG
  AAAACGAACACTCCCCCCAACACATTTTTTTTATGGATTTACCCATTTAACACCTTCACC
  CATCCACTGGGTTTTTAGATCTATTCGGAACTGTAATTCCCACTCCAATTTGCTTGTTTT
  CCTTAAGCACTTTTCACAACAGTGTACACCCTTCTACACTCTCTTCCCACCCTTCCAACC
  ATTTTGTAAGTACTCGGGCCCTTTAAGGCTTAACCTATAGACTCGGGTCCTGGTACCGCC
  CCAACAGTGTTTTATTTCACCTTTCCTGGCCTTAATTCCTGGGCACACCCCAACCGAGTT
  CTCTCAAGTGGACCACAACCCCCCTCTTTTCGAAGGAAAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003850
• Insert Size: 2090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003850.1, NP_003841.1
• RefSeq Size: 2178 bp
• RefSeq ORF: 1392 bp
• Locus ID: 8803
• UniProt ID: Q9P2R7
• Cytogenetics: 13q14.2
• Domains: ATP-grasp, ligase-CoA
• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways, Propanoate metabolism
• Gene Summary: Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6. [provided by RefSeq, Jul 2008]