Plakophilin 2 (PKP2) (NM_004572) Human Untagged Clone

CAT#: SC117285

PKP2 (untagged)-Human plakophilin 2 (PKP2), transcript variant 2b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Plakophilin 2
• Synonyms: ARVD9
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004572, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAGCCCCCGGCGCCCCAGCTGAGTACGGCTACATCCGGACCGTCCTGGGCCAGCAGATCCTGGGAC
  AACTGGACAGCTCCAGCCTGGCGCTGCCCTCCGAGGCCAAGCTGAAGCTGGCGGGGAGCAGCGGCCGCGG
  CGGCCAGACAGTCAAGAGCCTGCGGATCCAGGAGCAGGTGCAGCAGACCCTCGCCCGGAAGGGCCGCAGC
  TCCGTGGGCAACGGAAATCTTCACCGAACCAGCAGTGTTCCTGAGTATGTCTACAACCTACACTTGGTTG
  AAAATGATTTTGTTGGAGGCCGTTCCCCTGTTCCTAAAACCTATGACATGCTAAAGGCTGGCACAACTGC
  CACTTATGAAGGTCGCTGGGGAAGAGGAACAGCACAGTACAGCTCCCAGAAGTCCGTGGAAGAAAGGTCC
  TTGAGGCATCCTCTGAGGAGACTGGAGATTTCTCCTGACAGCAGCCCGGAGAGGGCTCACTACACGCACA
  GCGATTACCAGTACAGCCAGAGAAGCCAGGCTGGGCACACCCTGCACCACCAAGAAAGCAGGCGGGCCGC
  CCTCCTAGTGCCACCGAGATATGCTCGTTCCGAGATCGTGGGGGTCAGCCGTGCTGGCACCACAAGCAGG
  CAGCGCCACTTTGACACATACCACAGACAGTACCAGCATGGCTCTGTTAGCGACACCGTTTTTGACAGCA
  TCCCTGCCAACCCGGCCCTGCTCACGTACCCCAGGCCAGGGACCAGCCGCAGCATGGGCAACCTCTTGGA
  GAAGGAGAACTACCTGACGGCAGGGCTCACTGTCGGGCAGGTCAGGCCGCTGGTGCCCCTGCAGCCCGTC
  ACTCAGAACAGGGCTTCCAGGTCCTCCTGGCATCAGAGCTCCTTCCACAGCACCCGCACGCTGAGGGAAG
  CTGGGCCCAGTGTCGCCGTGGATTCCAGCGGGAGGAGAGCGCACTTGACTGTCGGCCAGGCGGCCGCAGG
  GGGAAGTGGGAATCTGCTCACTGAGAGAAGCACTTTCACTGACTCCCAGCTGGGGAATGCAGACATGGAG
  ATGACTCTGGAGCGAGCAGTGAGTATGCTCGAGGCAGACCACATGCTGCCATCCAGGATTTCTGCTGCAG
  CTACTTTCATACAGCACGAGTGCTTCCAGAAATCTGAAGCTCGGAAGAGGGTTAACCAGCTTCGTGGCAT
  CCTCAAGCTTCTGCAGCTCCTAAAAGTTCAGAATGAAGACGTTCAGCGAGCTGTGTGTGGGGCCTTGAGA
  AACTTAGTATTTGAAGACAATGACAACAAATTGGAGGTGGCTGAACTAAATGGGGTACCTCGGCTGCTCC
  AGGTGCTGAAGCAAACCAGAGACTTGGAGACTAAAAAACAAATAACAGACCATACAGTCAATTTAAGAAG
  TAGGAATGGCTGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGCGG
  ATCACGAGGTCAGGAGTTCGAGACCAGCCTGACCAACATGGTTTGCTGTGGAATTTGTCATCTAATGACA
  AACTCAAGAATCTCATGATAACAGAAGCATTGCTTACGCTGACGGAGAATATCATCATCCCCTTTTCTGG
  GTGGCCTGAAGGAGACTACCCAAAAGCAAATGGTTTGCTCGATTTTGACATATTCTACAACGTCACTGGA
  TGCCTAAGAAACATGAGTTCTGCTGGCGCTGATGGGAGAAAAGCGATGAGAAGATGTGACGGACTCATTG
  ACTCACTGGTCCATTATGTCAGAGGAACCATTGCAGATTACCAGCCAGATGACAAGGCCACGGAGAATTG
  TGTGTGCATTCTTCATAACCTCTCCTACCAGCTGGAGGCAGAGCTCCCAGAGAAATATTCCCAGAATATC
  TATATTCAAAACCGGAATATCCAGACTGACAACAACAAAAGTATTGGATGTTTTGGCAGTCGAAGCAGGA
  AAGTAAAAGAGCAATACCAGGACGTGCCGATGCCGGAGGAAAAGAGCAACCCCAAGGGCGTGGAGTGGCT
  GTGGCATTCCATTGTTATAAGGATGTATCTGTCCTTGATCGCCAAAAGTGTCCGCAACTACACACAAGAA
  GCATCCTTAGGAGCTCTGCAGAACCTCACGGCCGGAAGTGGACCAATGCCGACATCAGTGGCTCAGACAG
  TTGTCCAGAAGGAAAGTGGCCTGCAGCACACCCGAAAGATGCTGCATGTTGGTGACCCAAGTGTGAAAAA
  GACAGCCATCTCGCTGCTGAGGAATCTGTCCCGGAATCTTTCTCTGCAGAATGAAATTGCCAAAGAAACT
  CTCCCTGATTTGGTTTCCATCATTCCTGACACAGTCCCGAGTACTGACCTTCTCATTGAAACTACAGCCT
  CTGCCTGTTACACATTGAACAACATAATCCAAAACAGTTACCAGAATGCACGCGACCTTCTAAACACCGG
  GGGCATCCAGAAAATTATGGCCATTAGTGCAGGCGATGCCTATGCCTCCAACAAAGCAAGTAAAGCTGCT
  TCCGTCCTTCTGTATTCTCTGTGGGCACACACGGAACTGCATCATGCCTACAAGAAGGCTCAGTTTAAGA
  AGACAGATTTTGTCAACAGCCGGACTGCCAAAGCCTACCACTCCCTTAAAGACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004572 unedited
  NTTTTTCGNAATTATGTATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCAG
  CTCGGTCGCCCCCACCGGCCCCATGGCAGCCCCCGGCGCCCCAGCTGAGTACGGCTACAT
  CCGGACCGTCCTGGGCCAGCAGATCCTGGGACAACTGGACAGCTCCAGCCTGGCGCTGCC
  CTCCGAGGCCAAGCTGAAGCTGGCGGGGAGCAGCGGCCGCGGCGGCCAGACAGTCAAGAG
  CCTGCGGATCCAGGAGCAGGTGCAGCAGACCCTCGCCCGGAAGGGCCGCAGCTCCGTGGG
  CAACGGAAATCTTCACCGAACCAGCAGTGTTCCTGAGTATGTCTACAACCTACACTTGGT
  TGAAAATGATTTTGTTGGAGGCCGTTCCCCTGTTCCTAAAACCTATGACATGCTAAAGGC
  TGGCACAACTGCCACTTATGAAGGTCGCTGGGGAAGAGGAACAGCACAGTACAGCTCCCA
  GAAGTCCGTGGAAGAAAGGTCCTTGAGGCATCCTCTGAGGAGACTGGAGATTTCTCCTGA
  CAGCAGCCCGGAGAGGGCTCACTACACGCACAGCGATTACCAGTACAGCCAGAGAAGCCA
  GGCTGGGCACACCCTGCACCACCAAGAAAGCAGGCGGGCCGCCCTCCTAGTGCCACCGAG
  ATATGCTCGTTCCGAGATCGTGGNGGTCAGCCGTGCTGGCACCACAAGCAGGCAGCGCCA
  CTNTGACACATACCACAGACAGTACCAGCATGGCTCTGTTAGCGACACCGTTTTTGACAG
  CATCCCTGCCAACCCGGCCCTGCTCACGTACCCCCAGCCAGGGACCAGCCGCAGCATGGG
  CAACCTCTGGNAGAAGGAGAACTACCTCGACGCAGGNCTCACTGTCGGCCCAGTCAGCCC
  GCTGGTGCCCCTGCAGCCGTCACTCAGAACAGGCTTTCCAGTCCTCCTGGCATAAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004572 unedited
  TACCTAGCTTCNNGTCNAGGTCGAGTTTATTACTTTTTTTTTTAAATTATCGAATGCTTT
  ATTAATGCCTTAAAAAACACAAAAGTTCTGAACACACATTTATTATCTGGAGGAATAAAT
  GCACTCCAAATGCTTATCATCAGAACTCTGGAAAAAGAATATAAATTCTCTCCAGTATTT
  TAAAAATTGGTATCTAAATAATTGCGGTGGGAACTAGGTTAGCAACCTAAGTTGGCTTCA
  TTCAGCTTTTAAAATTGAAAAAAAATTGCTTCAAATATAATAAAGACTTAAGTACACATT
  CAAAATTTATTTTCTGTTACAGTTTTCTTGCCAAAAGGAGTTGAAGAATTTTTTTGCTTA
  TCTTACTTAGGACAAATGTTGTCAGATTAAAAAAAAAAATAACAGAATAGTAGTTGTAAA
  GACTAACTCTATATGAATGGTTCTAATCATAGCGCAGTTAAAACAACATTTAATGTACAT
  TCATTTCGCTGGATGATGTCACCCATTTAAAATATCACGATTTCCCATATCATCGCTGGA
  TGGTCTTGACACATTCTTACATCTTTTTCAACATGACTTCTTCCACTTTCCTTCTCTAGA
  GTTTTCTTGAGTTAAAATACTGGTCTTGCAGAAAACATTTGCCATCCAGCGGCCCCGCGA
  TATTTGTTAACCGGCGGGGCAAGGCTGCGCTGGCTGCCCATGCCCCTCATTATCCTGAAC
  CCGCTTTCCCCCGCCCCACGCTGGCCCCCCTCCCTGTTCCCGGGTCTCCCTCCTTTTCCC
  CCTCCCATTCGATTCTTCTTTTTCCTATTCCCGGCCTCGCCCCCCGACCCCCCACCCGCC
  TCCGTCTTATCCCCCGCCCGCGACCATTATGCCCCCACGTTTCTTTCCCGCCTCCCCTTC
  CCCCAATTTGTCTTTTTTTTTCTGCCCCCCCCCCGTCCCTTTTCCGCCCCTTTTTGTTCA
  TTCCCCCCCTCAATCCACACCTAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004572
• Insert Size: 4230 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004572.2, NP_004563.1
• RefSeq Size: 4439 bp
• RefSeq ORF: 2646 bp
• Locus ID: 5318
• UniProt ID: Q99959
• Cytogenetics: 12p11.21
• Domains: Armadillo_seg
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC)
• Gene Summary: This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This gene product may regulate the signaling activity of beta-catenin. Two alternately spliced transcripts encoding two protein isoforms have been identified. A processed pseudogene with high similarity to this locus has been mapped to chromosome 12p13. [provided by RefSeq, Jul 2008]
  Transcript Variant: This transcript variant (2b) represents the longer transcript variant and encodes the longer isoform.