Axin 2 (AXIN2) (NM_004655) Human Untagged Clone

CAT#: SC117255

AXIN2 (untagged)-Human axin 2 (AXIN2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Axin 2
• Synonyms: AXIL; ODCRCS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >SC117255 representing NM_004655.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  CTCGCCGGGCGCCTGCCCCCTCCTCGGGGGCAAAGGCTTTGTGACCAAGCAGACGACGAAGCATGTCCA
  CCACCACTACATCCACCACCATGCCGTCCCCAAGACCAAGGAGGAGATCGAGGCGGAGGCCACGCAGCG
  GGTGCACTGCTTCTGCCCTGGGGGCAGCGAGTATTACTGCTACTCGAAATGCAAAAGCCACTCCAAGGC
  TCCGGAAACCATGCCCAGCGAGCAGTTTGGCGGCAGCAGAGGCAGTACCTTGCCCAAACGCAATGGGAA
  AGGCACGGAGCCGGGCCTGGCCCTGCCCGCCAGGGAAGGAGGGGCCCCCGGCGGAGCTGGGGCCCTGCA
  GCTTCCCCGGGAGGAAGGAGACAGGTCGCAGGATGTCTGGCAGTGGATGCTGGAGAGTGAGCGGCAGAG
  CAAGCCCAAGCCCCATAGTGCCCAAAGCACAAAAAAGGCCTACCCCTTGGAGTCTGCCCGCTCGTCTCC
  AGGCGAACGAGCCAGCCGGCACCATCTGTGGGGGGGCAACAGCGGGCACCCCCGCACCACCCCCCGTGC
  CCACCTGTTCACCCAGGACCCTGCGATGCCTCCCTTGACCCCACCCAACACGCTGGCTCAGCTGGAGGA
  GGCCTGTCGCAGGCTAGCTGAGGTGTCGAAGCCCCCAAAGCAGCGGTGCTGTGTGGCCAGTCAGCAGAG
  GGACAGGAATCATTCGGCCACTGTTCAGACGGGAGCCACACCCTTCTCCAATCCAAGCCTGGCTCCAGA
  AGATCACAAAGAGCCAAAGAAACTGGCAGGTGTCCACGCGCTCCAGGCCAGTGAGTTGGTTGTCACTTA
  CTTTTTCTGTGGGGAAGAAATTCCATACCGGAGGATGCTGAAGGCTCAGAGCTTGACCCTGGGCCACTT
  TAAAGAGCAGCTCAGCAAAAAGGGAAATTATAGGTATTACTTCAAAAAAGCAAGCGATGAGTTTGCCTG
  TGGAGCGGTGTTTGAGGAGATCTGGGAGGATGAGACGGTGCTCCCGATGTATGAAGGCCGGATTCTGGG
  CAAAGTGGAGCGGATCGATTGAGCCCTGGGGTCTGGCTTTGGTGAACTGTTGGAGCCCGAAGCTCTTGT
  GAACTGTCTTGGCTGTGAGCAACTGCGACAAAACATTTTGAAGGAAAATTAAACCAATGAAGAAGACAA
  AGTCTAAGGAAGAATCGGCCAGTGGGCCTTCGGGAGGGCGGGGGGAGGTTGATTTTCATGATTCATGAG
  CTGGGTACTGACTGAGATAAGAAAAGCCTGAACTATTTATTAAAAACATGACCACTCTTGGCTATTGAA
  GATGCTGCCTGTATTTGAGAGACTGCCATACATAATATATGACTTCCTAGGGATCTGAAATCCATAAAC
  TAAGAGAAACTGTGTATAGCTTACCTGAACAGGAATCCTTACTGATATTTATAGAACAGTTGATTTCCC
  CCATCCCCAGTTTATGGATATGCTGCTTTAAACTTGGAAGGGGGAGACAGGAAGTTTTAATTGTTCTGA
  CTAAACTTAGGAGTTGAGCTAGGAGTGCGTTCATGGTTTCTTCACTAACAGAGGAATTATGCTTTGCAC
  TACGTCCCTCCAAGTGAAGACAGACTGTTTTAGACAGACTTTTTAAAATGGTGCCCTACCATTGACACA
  TGCAGAAATTGGTGCGTTTTGTTTTTTTTTTTCCTATGCTGCTCTGTTTTGTCTTAAAGGTCTTGAGGG
  TTGACCATGTTGCGTCATCATCAACATTTTGGGGGTTGTGTTGGATGGGATGATCTGTTGCAGAGGGAG
  AGGCAGGGAACCCTGCTCCTTCGGGCCCCAGGTTGATCCTGTGACTGAGGCTCCCCCTCATGTAGCCTC
  CCCAGGCCCAGGGCCCTGAGGCCTGCTAGAATCACTGCCGCTGTGCTTTCGTGGAAATGACAGTTCCTT
  GTTTTTTTTGTTTCTGTTTTTGTTTTACATTAGTCATTGGACCACAGCCATTCAGGAACTACCCCCTGC
  CCCACAAAGAAATGAACAGTTGTAGGGAGACCCAGCAGCACCTTTCCTCCACACACCTTCATTTTGATG
  TTCGGGTTTTTGTGTTAAGTTAATCTGTACATTCTGTTTGCCATTGTTACTTGTACTATACATCTGTAT
  ATAGTGTACGGCAAAAGAGTATTAATCCACTATCTCTAGTGCTTGACTTTAAATCAGTACAGTACCTGT
  ACCTGCACGGTCACCCGCTCCGTGTGTCGCCCTATATTGAGGGCTCAAGCTTTCCCTTGTTTTTTGAAA
  GGGGTTTATGTATAAATATATTTTATGCCTTTTTATTACAAGTCTTGTACTCAATGACTTTTGTCATGA
  CATTTTGTTCTACTTATACTGTAAATTATGCATTATAAAGAGTTCATTTAAGGAAAATTACTTGGTACA
  ATAATTATTGTAATTAAGAGATGTAGCCTTTATTAAAATTTTATATTTTTCAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004655 unedited
  TGGCGGCACAGCAATTCGGCACGAGGGCCCGAGTAGCCGGGNAAATAAAAATAACCCCTC
  AGAGCGATGGATTTCGGGGCCGCCCGGCGGCCGAGGCGCCCGCCGAAGGCCCTGCTGTAA
  AAGAGAGGAGGTTCAGATGAGCCCCTGCTGACTTGAGAGAGACAGAGAGACCACGCCGAT
  TGCTGAGAGGTACTATGAAGAAGAAAAATTCCCAGACTCAGTGGGAAGAGCTCCCTCACC
  ATGAGTAGCGCTATGTTGGTGACTTGCCTCCCGGACCCCAGCAGCAGCTTCCGTGAGGAT
  GCCCCGCGGCCCCCAGTGCCAGGGGAAGAAGGGGAGACCCCACCGTGTCAGCCAGGGATG
  GGCAAGGGCCAGGTCACCAAACCCATGCCTGTCTCTTCCAACACCAGGCGGAACGAAGAT
  GGGTTGGGGGAGCCGGAGGGGCGGGCATCTCCGGATTCCCCTCTGACCCGGTGGACCAAG
  TCCTTACACTCCTTATTGGGCGATCAAGACGGTGCTTACCTGTTCCGAACTTACTCGGAG
  AGGGAGAAATGCGTGGATACCTTAAACTTCTGGTTTGCCTGCAATGGATTCAGGCAGATG
  AACCTGAACGATACCCAAACTTTTCCAGGTGGCAAAGCGATACTACAAAAGGTACTTTGG
  GAACAACAGCATTGTTTTCAAACGATTTAAGCCTGCCCCAAAACCTACTTAAAGAAGGCA
  TCAAGGAGCAGCAGGATGGTTCCCTCAATGTTATCCACGCCCCAGACCTGGATTCCACTC
  GCGGATTGGGAGGACAATGCCCTTCCACAGTTGCTGGGCCTCTTGTTTTTTCTCCTCGAT
  TTTCGTGGGGGCGGCGGCGAAAACACCCTTTTCTTTCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004655 unedited
  TAGCTCTGGACCGCGGCACGCAATCTAGTGATCGGTTTTTTTTTTTTTTTTTTGAAAAAA
  TAAAATTTTAATAAAGGCTACATCTCTTAATTACAATAATTATTGTACCAAGTAATTTTC
  CTTAAATGAACTCTTTATAATGCATAATTTACAGTATAAGTAGAACAAAATGTCATGACA
  AAAGTCATTGAGTACAAGACTTGTAATAAAAAGGCATAAAATATATTTATACATAAACCC
  CTTTCAAAAAACAAGGGAAAGCTTGAGCCCTCAATATAGGGCGACACACGGAGCGGGTGA
  CCGTGCAGGTACAGGTACTGTACTGATTTAAAGTCAAGCACTAGAGATAGTGGATTAATA
  CTCTTTTGCCGTACACTATATACAGATGTATAGTACAAGTAACAATGGCAAACAGAATGT
  ACAGATTAACTTAACACAAAAACCCGAACATCAAAATGAAGGTGTGTGGAGGAAAGGTGC
  TGCTGGGTCTCCCTACAACTGTTCATTTCTTTGTGGGGCAGGGGGTAGTTCCTGAATGGC
  TGTGGTCCAATGACTAATGTAAAACAAAAACAGAAACAAAAAAAACAAGGAACTGTCATT
  TCCACGAAAGCACAGCGGCAGTGATTCTAGCAGGCCTCAGGGCCCTGGGCCTGGGGAGGC
  TACATGAGGGGGAGCCTCAGTCACAGGATCAACCTGNGGCCCGAATGAGCAGGGTTCCCT
  GCCTCTCCCTCTGCAACAGATCATCCCATCCAACACAACCCCCAAATGTTGATGATGACG
  CAACATGGTCAACCCTCAAGACCTTTAAGACAAACAGAGCAGCCTAGGAAAAAAAAAACA
  AAACGCACCAATTTCTGCATGTGTCAATGGTAGGGCACCATTTTAAAAAGTCTGTCTAAA
  CAGTCTGTCTCACCTGGNAGGACGANTGCAAGCATAATTCTCTGTTATGAAGAACATGAA
  CCACCCTAGCTAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004655
• Insert Size: 4156 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004655.2
• RefSeq Size: 4259 bp
• RefSeq ORF: 4156 bp
• Locus ID: 8313
• UniProt ID: Q9Y2T1
• Cytogenetics: 17q24.1
• Domains: RGS, DAX
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Induced pluripotent stem cells
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Pathways in cancer, Wnt signaling pathway
• MW: 153.3 kDa
• Gene Summary: The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq, Jul 2008]