COX7A2L (NM_004718) Human Untagged Clone

CAT#: SC117225

COX7A2L (untagged)-Human cytochrome c oxidase subunit VIIa polypeptide 2 like (COX7A2L), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COX7A2L
• Synonyms: COX7AR; COX7RP; EB1; SCAF1; SCAFI; SIG81
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117225 sequence for NM_004718 edited (data generated by NextGen Sequencing)
  ATGTACTACAAGTTTAGTGGCTTCACGCAGAAGTTGGCAGGAGCATGGGCTTCGGAGGCC
  TATAGCCCGCAGGGATTAAAGCCTGTGGTTTCCACAGAAGCACCACCTATCATATTTGCC
  ACACCAACTAAACTGACCTCCGATTCCACAGTGTATGATTATGCTGGGAAAAACAAAGTT
  CCAGAGCTACAAAAGTTTTTCCAGAAAGCTGATGGTGTGCCCGTCTACCTGAAACGAGGC
  CTGCCTGACCAAATGCTTTACCGGACCACCATGGCGCTGACTGTGGGAGGGACCATCTAC
  TGCCTGATCGCCCTCTACATGGCTTCGCAGCCCAAAAACAAATGA
  
  Clone variation with respect to NM_004718.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004718 unedited
  AAGTCAGAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGTCCTTCT
  CTGGGGCGGTCGCGTTGGCAGCGGATGCGGGAAGCCGGACTCTGGGCGTCATGTACTACA
  AGTTTAGTGGCTTCACGCAGAAGTTGGCAGGAGCATGGGCTTCGGAGGCCTATAGCCCGC
  AGGGATTAAAGCCTGTGGTTTCCACAGAAGCACCACCTATCATATTTGCCACACCAACTA
  AACTGACCTCCGATTCCACAGTGTATGATTATGCTGGGAAAAACAAAGTTCCAGAGCTAC
  AAAAGTTTTTCCAGAAAGCTGATGGTGTGCCCGTCTACCTGAAACGAGGCCTGCCTGACC
  AAATGCTTTACCGGACCACCATGGCGCTGACTGTGGGAGGGACCATCTACTGCCTGATCG
  CCCTCTACATGGCTTCGCAGCCCAAAAACAAATGAGTTAGGCTGCAGAGGACTGGTTTGT
  TTTTTGGCATAAACCCTTTGAAGTTCCTTTTTCATTGTTAAATTAAAATTTTTTTTTTTA
  CTTGGATGGCTTAACATTTTTGCAAGAAAAATAGGAAGATATGAAGATGATGTTNTTGGT
  TTGTTTATGAAATGCATATGGCTTGTCAGAGCTCATTCGACAGTTAAAGCCATTGTTTAA
  AGAAATGGTGCTTTGCTCTGTGTTTGTGCTCCTGATTTCCCTGGAGGTTCTGGATGAAGG
  CTGAACACAGGCTTGTTAATGTCAGTCTGTGCTGAGGACCTCAGGACNTTGAGGTTGCAT
  TTTTGAGCATGGGGTGCANGAGCCTTTCTGGATTTTGGATGTGGCTATNGGAAAGAACAC
  AGAGCAAGGTCATGTGCATGAATGGAGGAGTTTGAGTTAGTCCCTCGGGGGATTTTTTCC
  ATTTTGCAGTAAATGTTAAATTAATGTACCTGCCTCTATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004718 unedited
  CGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTAATTTTTCATTCATGTATTTA
  TTCCACAGTCAAAATAAATCAAAATTTAAAGCTATAACATTTTTAAAAGATAAAGGAGAA
  TTTGTGGCACAGCTGCATTAACAAAACAGACACCAGTCTAAAGTGCAACACTAAACAGGT
  ATTCTCTGTTCCCACGGTGGAATAATTACACATAAGATTTCACTAAAGATAGGAGATGAG
  GCAAATAACCCTTTGAAATTACCTGCCCAACAAATAGAGGCAGGCTACATTAATTTAACA
  TTTTACTGCAAAATGGAAAAAATCCCCGAGGTGACTAACTCAAACTCCTCATTTCATGCA
  CATGACCTTGGCTTCTGTGTTCTTTCCATAGCCACATCCAAATCCAGAAAGGCTCCTGCA
  CCCCATGCTCAAAAATGCAACCTCAAGTCCCTGAGGTCCTCAGCACAGACTGACATTAAC
  AAGCCTGTGTTCAGCCTTCATCCAGAACCTCCAGGGAAATCAGGAGCACAAACACAGAGC
  AAAGCACCATTTCTTTAAACAATGGCTTTAACTGTCGAATGAGCTCTGACAAGCCATATG
  CATTTCATAAACAAACCAAAACATCATCTTCATATCTTCCTATTTTTCTTGCAAAAATGT
  TAAGCCATCCAANGTAAAAAAAAAATTTAATTTAACAATGAAAAAGGAACTTNCAAGGGT
  TTATGCCAAAAAACAAACCAGTCCTCTGCAGCCTAACTCATTTGTTTTTGGGGCTGCGAA
  GCCATGTAGAGGGCGATCAGGCAGTAGATGGTCCCCTCCACAGTCAGCGCCATGGTGGTC
  CCGTTAAGCATTTGGTCAGGCAGCCCTCGTTTCAGTACACGGCCACACCATCAAGCTTCT
  GGAAACACTTTTGTAGCTCTGGAACTCTGGTTTTCCACCCATATCTACCCTGGGGATCGG
  AGTCATTTTATTTGTGTGGCAAAATGATAGGCGTGCTCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004718
• Insert Size: 1110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004718.2, NP_004709.2
• RefSeq Size: 1145 bp
• RefSeq ORF: 345 bp
• Locus ID: 9167
• UniProt ID: O14548
• Cytogenetics: 2p21
• Domains: COX7a
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein similar to polypeptides 1 and 2 of subunit VIIa in the C-terminal region, and also highly similar to the mouse Sig81 protein sequence. This gene is expressed in all tissues, and upregulated in a breast cancer cell line after estrogen treatment. It is possible that this gene represents a regulatory subunit of COX and mediates the higher level of energy production in target cells by estrogen. Several transcript variants, some protein-coding and others non-protein coding, have been found for this gene. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes the longest isoform (a). Variants 1 and 2 both encode the same isoform (a).