GLUD1 (NM_005271) Human Untagged Clone

CAT#: SC116830

GLUD1 (untagged)-Human glutamate dehydrogenase 1 (GLUD1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GLUD1
• Synonyms: GDH; GDH1; GLUD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116830 sequence for NM_005271 edited (data generated by NextGen Sequencing)
  ATGTACCGCTACCTGGGCGAAGCGCTGTTGCTGTCCCGGGCCGGGCCCGCTGCCCTGGGC
  TCGGCGTCCGCCGACTCGGCCGCGTTGCTGGGCTGGGCCCGGGGACAGCCCGCCGCCGCC
  CCGCAGCCGGGGCTGGCATTGGCCGCCCGGCGCCACTACAGCGAGGCGGTGGCCGACCGC
  GAGGACGACCCCAACTTCTTCAAGATGGTGGAGGGCTTCTTCGATCGCGGCGCCAGCATC
  GTGGAGGACAAGCTGGTGGAGGACCTGAGGACCCGGGAGAGCGAGGAGCAGAAGCGGAAC
  CGGGTGCGCGGCATCCTGCGGATCATCAAGCCCTGCAACCATGTGCTGAGTCTCTCCTTC
  CCCATCCGGCGCGACGACGGCTCCTGGGAGGTCATCGAAGGCTACCGGGCCCAGCACAGC
  CAGCACCGCACGCCCTGCAAGGGAGGTATCCGTTACAGCACTGATGTGAGTGTAGATGAA
  GTAAAAGCTTTGGCTTCTCTGATGACATACAAGTGTGCAGTGGTTGATGTGCCGTTTGGG
  GGTGCTAAAGCTGGTGTTAAGATCAATCCCAAGAACTATACTGATAATGAATTGGAAAAG
  ATCACAAGGAGGTTCACCATGGAGCTAGCAAAAAAGGGCTTTATTGGTCCTGGCATTGAT
  GTGCCTGCTCCAGACATGAGCACAGGTGAGCGGGAGATGTCCTGGATCGCTGATACCTAT
  GCCAGCACCATAGGGCACTATGATATTAATGCACACGCCTGTGTTACTGGTAAACCCATC
  AGCCAAGGGGGAATCCATGGACGCATCTCTGCTACTGGCCGTGGTGTCTTCCATGGGATT
  GAAAATTTCATCAATGAAGCTTCTTACATGAGCATTTTAGGAATGACACCAGGGTTTGGA
  GATAAAACATTTGTTGTTCAGGGATTTGGTAATGTGGGCCTACACTCTATGAGATATTTA
  CATCGTTTTGGTGCTAAATGTATTGCTGTTGGTGAGTCTGATGGGAGTATATGGAATCCA
  GATGGTATTGACCCAAAGGAACTGGAAGACTTCAAATTGCAACATGGGTCCATTCTGGGC
  TTCCCCAAGGCAAAGCCCTATGAAGGAAGCATCTTGGAGGCCGACTGTGACATACTGATC
  CCAGCTGCCAGTGAGAAGCAGTTGACCAAATCCAACGCACCCAGAGTCAAAGCCAAGATC
  ATTGCTGAAGGTGCCAATGGGCCAACAACTCCAGAAGCTGACAAGATCTTCCTGGAGAGA
  AACATTATGGTTATTCCAGATCTCTACTTGAATGCTGGAGGAGTGACAGTATCTTACTTT
  GAGTGGCTGAAGAATCTAAATCATGTCAGCTATGGCCGTTTGACCTTCAAATATGAAAGG
  GATTCTAACTACCACTTGCTCATGTCTGTTCAAGAGAGTTTAGAAAGAAAATTTGGAAAG
  CATGGTGGAACTATTCCCATTGTACCCACGGCAGAGTTCCAAGACAGGATATCGGGTGCA
  TCTGAGAAAGACATCGTGCACTCTGGCTTGGCATACACAATGGAGCGTTCTGCCAGGCAA
  ATTATGCGCACAGCCATGAAGTATAACCTGGGATTGGACCTGAGAACAGCTGCCTATGTT
  AATGCCATTGAGAAAGTCTTCAAAGTGTACAATGAAGCTGGTGTGACCTTCACATAG
  
  Clone variation with respect to NM_005271.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005271 unedited
  TGTCAGAATATTTGTATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCGCGC
  CTGTTTCGCGACCATCACGCACCTCCCCTCCGCTTGTGGCCATGTACCGCTACCTGGGCG
  AAGCGCTGTTGCTGTCCCGGGCCGGGCCCGCTGCCCTGGGCTCGGCGTCCGCCGACTCGG
  CCGCGTTGCTGGGCTGGGCCCGGGGACAGCCCGCCGCCGCCCCGCAGCCGGGGCTGGCAT
  TGGCCGCCCGGCGCCACTACAGCGAGGCGGTGGCCGACCGCGAGGACGACCCCAACTTCT
  TCAAGATGGTGGAGGGCTTCTTCGATCGCGGCGCCAGCATCGTGGAGGACAAGCTGGTGG
  AGGACCTGAGGACCCGGGAGAGCGAGGAGCAGAAGCGGAACCGGGTGCGCGGCATCCTGC
  GGATCATCAAGCCCTGCAACCATGTGCTGAGTCTCTCCTTCCCCATCCGGCGCGACGACG
  GCTCCTGGGAGGTCATCGAAGGCTACCGGGCCCAGCACAGCCAGCACCGCACGCCCTGCA
  AGGGAGGTATCCGTTACAGCACTGATGTGAGTGTAGATGAAGTAAAAGCTTTGGCTTCTC
  TGATGACATACAAGTGTGCAGTGGTTGATGTGCCGTTTGGGGGTGCTAAAGCTGGTGTTA
  AGATCAATCCCAAGAACTATACTGATAATGAATTGGAAAAGATCACAAGGAGGTTCACCA
  TGGAGCTAGCAAAAAACGGCTTTATTGGTCCTGGCATTGATGTGCCTGCTCCAGACTTGA
  GCACAGGTGAGCGGGAGATGTCCTGGATCGCTGATACCTATGCCCACCACACTAGGCCCT
  ATGATATTATGCCCCCCCTGTGTTACTGTGAACCCATCACCAAGGGGGATCCATGACGCA
  TCTCTGCTACTGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005271 unedited
  TGGACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCTGTTCATATTA
  TTTAATAAAATACAAAATAATTCGAGAATAAAGACTATGCTTTCAGGGATCATTTCTATA
  GTTCGTTACTCGGGAAGTTTCTCTGAACGTGTAAAGCACCGAACAAAAAAAAAAAAAACA
  ATTCAAGAATAAAATCTGGGCAGCTCACAATAAAGTGGAAGGAAATGTTATACAGGTGGT
  CGCTATAAACATTTCAGAAATCCAATTGCAGTATTATAACTTATGAAAAGAGAAACTACC
  ACATGTAAAAACACAGAAAAGAAACGTAAGTTTTGGAAAACGTAGACTTTTAATAACTGC
  TTTTATCACCAGGTTAAGCCATGCAGTTACAAAGTAGTTAGAAATTTCTGAAGGGTGTTA
  GAGTTAAAAAAAAAAAAAAAGCTGACTCTTACACATATATTATCTAGCACTTCATGGGGA
  CACTACTGTTCAAAAGGCCCTGGCCAAATAACTCCCAAATGAAACACTCAACCCAAGGAT
  GTTTTCAGCCCACTGTTAGTGAAGCTGGGTGCAGAATGCAAAGCCTCTAAAAGGAGAGGA
  TACAAAGTCAGGTGAGTAGGGGCCATTGGCAATGCTCAGAGCCAGCCAGACTCCAAACAG
  GGAGCCCAAGTGGGTTTTTTCTGGGACACTCTACTTGAANNTATTGTTAATTAGTCAACC
  ATAGATCTTCAAAAGAGAACAATTAGTTAACATGATAAAAAAGTGACTGCATTNCTTGGN
  ACTGGATCATAANTATAGTAAGCAAATTCTGACAGTTTTAATGAAAAATAGCCTNCACCA
  TTTTGCAGGTATGAAGATAACTTATNTATNCCCAGGNCTTNTNATGTCTGCTGGACANTA
  CATGGATGATAGCTATAATGCACATGNTTATGAATAAAAAACTAGNTTTTNCATTATATT
  TCTTAGGCATCACTGTTGAGTCAGGTAGCTTGTTAGGATAAA
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_005271
• Insert Size: 3370 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005271.1, NP_005262.1
• RefSeq Size: 3051 bp
• RefSeq ORF: 1677 bp
• Locus ID: 2746
• UniProt ID: P00367
• Cytogenetics: 10q23.2
• Domains: GLFV_dehydrog, GLFV_dehydrog_N
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism
• Gene Summary: This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) encodes the longest isoform (a).