GLCNE (GNE) (NM_005476) Human Untagged Clone

CAT#: SC116716

GNE (untagged)-Human glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GLCNE
• Synonyms: DMRV; GLCNE; IBM2; NM; Uae1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116716 sequence for NM_005476 edited (data generated by NextGen Sequencing)
  ATGGAGAAGAATGGAAATAACCGAAAGCTGCGGGTTTGTGTTGCTACTTGTAACCGTGCA
  GATTATTCTAAACTTGCCCCGATCATGTTTGGCATTAAAACCGAACCTGAGTTCTTTGAA
  CTTGATGTTGTGGTACTTGGCTCTCACCTGATAGATGACTATGGAAATACATATCGAATG
  ATTGAACAAGATGACTTTGACATTAACACCAGGCTACACACAATTGTGAGGGGAGAAGAT
  GAGGCAGCCATGGTGGAGTCAGTAGGCCTGGCCCTAGTGAAGCTGCCAGATGTCCTTAAT
  CGCCTGAAGCCTGATATCATGATTGTTCATGGAGACAGGTTTGATGCCCTGGCTCTGGCC
  ACATCTGCTGCCTTGATGAACATCCGAATCCTTCACATTGAAGGTGGGGAAGTCAGTGGG
  ACCATTGATGACTCTATCAGACATGCCATAACAAAACTGGCTCATTATCATGTGTGCTGC
  ACCCGCAGTGCAGAGCAGCACCTGATATCCATGTGTGAGGACCATGATCGCATCCTTTTG
  GCAGGCTGCCCTTCCTATGACAAACTTCTCTCAGCCAAGAACAAAGACTACATGAGCATC
  ATTCGCATGTGGCTAGGTGATGATGTAAAATCTAAAGATTACATTGTTGCACTACAGCAC
  CCTGTGACCACTGACATTAAGCATTCCATAAAAATGTTTGAATTAACATTGGATGCACTT
  ATCTCATTTAACAAGCGGACCCTAGTCCTGTTTCCAAATATTGACGCAGGGAGCAAAGAG
  ATGGTTCGAGTGATGCGGAAGAAGGGCATTGAGCATCATCCCAACTTTCGTGCAGTTAAA
  CACGTCCCATTTGACCAGTTTATACAGTTGGTTGCCCATGCTGGCTGTATGATTGGGAAC
  AGCAGCTGTGGGGTTCGAGAAGTTGGAGCTTTTGGAACACCTGTGATCAACCTGGGAACA
  CGTCAGATTGGAAGAGAAACAGGGGAGAATGTTCTTCATGTCCGGGATGCTGACACCCAA
  GACAAAATATTGCAAGCACTGCACCTTCAGTTTGGTAAACAGTACCCTTGTTCAAAGATA
  TATGGGGATGGAAATGCTGTTCCAAGGATTTTGAAGTTTCTCAAATCTATCGATCTTCAA
  GAGCCACTGCAAAAGAAATTCTGCTTTCCTCCTGTGAAGGAGAATATCTCTCAAGATATT
  GACCATATTCTTGAAACTCTAAGTGCCTTGGCCGTTGATCTTGGCGGGACGAACCTCCGA
  GTTGCAATAGTCAGCATGAAGGGTGAAATAGTTAAGAAGTATACTCAGTTCAATCCTAAA
  ACCTATGAAGAGAGGATTAATTTAATCCTACAGATGTGTGTGGAAGCTGCAGCAGAAGCT
  GTAAAACTGAACTGCAGAATTTTGGGAGTAGGCATTTCCACAGGTGGCCGTGTAAATCCT
  CGGGAAGGAATTGTGCTGCATTCAACCAAACTGATCCAAGAGTGGAACTCTGTGGACCTT
  AGGACCCCCCTTTCTGACACTTTGCATCTCCCTGTGTGGGTAGACAATGATGGCAACTGT
  GCTGCCCTGGCGGAAAGGAAATTTGGCCAAGGAAAGGGACTGGAAAACTTTGTTACACTT
  ATCACAGGCACAGGAATCGGTGGTGGAATTATCCATCAGCATGAATTGATCCACGGAAGC
  TCCTTCTGTGCTGCAGAACTGGGCCACCTTGTTGTGTCTCTGGATGGGCCTGATTGTTCC
  TGTGGAAGCCATGGGTGCATTGAAGCATACGCCTCTGGAATGGCCTTGCAGAGGGAGGCA
  AAAAAGCTCCATGATGAGGACCTGCTCTTGGTGGAAGGGATGTCAGTGCCAAAAGATGAG
  GCTGTGGGTGCGCTCCATCTCATCCAAGCTGCGAAACTTGGCAATGCGAAGGCCCAGAGC
  ATCCTAAGAACAGCTGGAACAGCTTTGGGTCTTGGGGTTGTGAACATCCTCCATACCATG
  AATCCCTCCCTTGTGATCCTCTCCGGAGTCCTGGCCAGTCACTATATCCACATTGTCAAA
  GACGTCATTCGCCAGCAGGCCTTGTCCTCCGTGCAGGACGTGGATGTGGTGGTTTCGGAT
  TTGGTTGACCCCGCCCTGCTGGGTGCTGCCAGCATGGTTCTGGACTACACAACACGCAGG
  ATCTACTAG
  
  Clone variation with respect to NM_005476.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005476 unedited
  CCCATTCCCCTCGAGGCTAAACCATTAAACGCTGCTCTGCTCATTATTTCATACTCTACT
  AGGGTACAGAGCTCGTGCTTCGGGATGGAAACCTATGGTTATCTGCAGAGGGAGTCATGC
  TTTCAAGGACCTCATGAACTCTATTTTAAGAACCTCTCAAAACGAAACAAGCTAAACTGN
  GNTTAAGAATGGAAATAACCTTTAGCTGCGGGTTTGTGTTGCTACTTGTAACCGTGCAGA
  TTATTCTAAACTTGCCCCTATTATGTTTGGCATTAAAACCGAACCTGAGTTCTTTGAACT
  TGATGTTGTGGTACTTGGCTCTCACCTGATAGATGACTATGGAAATACATATCGAATGAT
  TGAACAAGATGACTTTGACATTAACACCAGGCTACACACAATTGTGAGGGGAGAAGATGA
  GGCAGCCATGGTGGAGTCAGTAGGCCTGGCCCTAGTGAAGCTGCCAGATGTCCTTAATCG
  CCTGAAGCCTGATATCATGATTGTTCATGGAGACAGGTTTGATGCCCTGGCTCTGGCCAC
  ATCTGCTGCCTTGATGAACATCCGAATCCTTCACATTGAAGGTGGGGAAGTCAGTGGGAC
  CATTGATGACTCTATCAGACATGCCATAACAAAACTGGCTCATTATCATGTGTGCTGCAC
  CCGCAGTGCATAGCAGCACCTGATATCCATGTGTGAGGACCATGATCGCATCCTTTTGGC
  AAGCTGCCCTTCCTATGACAAACTTCTCTCAGCCAAGACAAAGACTACCTGAGCATCATT
  CGCATGTGGCTAAGTGATGATGTAAAATCTAAAGATACATTGTTGCACTACGCACCCTGT
  GACCACTGACTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005476 unedited
  NAATCCTGNACGCGGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTAGTAGAGACG
  GGGTTTCACCATGTTTCACCATGTTAGCCAGGGCGGTCTCGATCTCCTGACCTCGTGATC
  CGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACTGTGCCCGGCCTG
  GAAGGATTATTAATGCAAACTTCAGGATGAAGTGATATCCCAGGCAAGGCCTGAAGGTCT
  CAGTGGTATTAATACATTAGTAAGCAGAGTTCTAAGAAGGCTTCCTCTCTATTATTGTAG
  CTGCTTTGGCACAGAAAATTGTGACTGTAACTTACAGGGTTTGAGCTAAAATGACCCCTA
  GTAAGAAGACATCAGAAAGAATAGCATCTACAAAAATAGGAGTGGGGAAAGGTGACTCTG
  GAAGAGGAGACCAAAAGTGAAAACATTTCTCTGCCAAAGTCACCTGCAGTTCAATACCAG
  ATTTGATTGTTAAGAAACGGTCATCCTAAAGACAAGAACTTGATTCCACTCAGGAGCTCT
  GGAGAGAAGGTCCATGTCTGTTCCTGGAGGTCTAGTAGATCCTGCGTGTTGTGTAGTCCA
  GAACCATGCTGGCAGCACCCANCAGGGCCGGGTCAACCAAATCCGAACCACCACATCCAC
  GTCCTGCACGGAGGACAAGGCCTGCTGGCGAATGACGTCTTTGACAATGGGGATATAGTG
  ACTGGCCCAGACTCCGGAGAGATCACCAGGGAGGAATTCTGGTTTGGGGGTGTTCACACC
  CCCAAAACCCAAGCTGTTCCGACCTTTCTTAGGAGCCCCGGGCCTTCCCCTTGCCAGTTT
  CCCAGCTCGGAGAGAAGGAGCCCCTCCCCGTGCTACATTTTTTTGCCGCAACGCCCTCCA
  CCCACACCGAGTACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005476
• Insert Size: 2940 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005476.3, NP_005467.1
• RefSeq Size: 3760 bp
• RefSeq ORF: 2169 bp
• Locus ID: 10020
• UniProt ID: Q9Y223
• Cytogenetics: 9p13.3
• Domains: ROK, Epimerase_2
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Metabolic pathways
• Gene Summary: The protein encoded by this gene is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells. Mutations in this gene are associated with sialuria, autosomal recessive inclusion body myopathy, and Nonaka myopathy. Alternative splicing of this gene results in transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) contains a different 5' terminal exon compared to transcript variant 1, which results in translation initiation from an in-frame downstream AUG, and a protein (isoform 2) with a shorter N-terminus compared to isoform 1. This variant has been called GNE1 in the scientific literature. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.