MDH1 (NM_005917) Human Untagged Clone

CAT#: SC116447

MDH1 (untagged)-Human malate dehydrogenase 1, NAD (soluble) (MDH1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MDH1
• Synonyms: DEE88; EIEE88; HEL-S-32; KAR; MDH-s; MDHA; MGC:1375; MOR2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116447 sequence for NM_005917 edited (data generated by NextGen Sequencing)
  ATGTCTGAACCAATCAGAGTCCTTGTGACTGGAGCAGCTGGTCAAATTGCATATTCACTG
  CTGTACAGTATTGGAAATGGATCTGTCTTTGGTAAAGATCAGCCTATAATTCTTGTGCTG
  TTGGATATCACCCCCATGATGGGTGTCCTGGACGGTGTCCTAATGGAACTGCAAGACTGT
  GCCCTTCCCCTCCTGAAAGATGTCATCGCAACAGATAAAGAAGACGTTGCCTTCAAAGAC
  CTGGATGTGGCCATTCTTGTGGGCTCCATGCCAAGAAGGGAAGGCATGGAGAGAAAAGAT
  TTACTGAAAGCAAATGTGAAAATCTTCAAATCCCAGGGTGCAGCCTTAGATAAATACGCC
  AAGAAGTCAGTTAAGGTTATTGTTGTGGGTAATCCAGCCAATACCAACTGCCTGACTGCT
  TCCAAGTCAGCTCCATCCATCCCCAAGGAGAACTTCAGTTGCTTGACTCGTTTGGATCAC
  AACCGAGCTAAAGCTCAAATTGCTCTTAAACTTGGTGTGACTGCTAATGATGTAAAGAAT
  GTCATTATCTGGGGAAACCATTCCTCGACTCAGTATCCAGATGTCAACCATGCCAAGGTG
  AAATTGCAAGGAAAGGAAGTTGGTGTTTATGAAGCTCTGAAAGATGACAGCTGGCTCAAG
  GGAGAATTTGTCACGACTGTGCAGCAGCGTGGCGCTGCTGTCATCAAGGCTCGAAAACTA
  TCCAGTGCCATGTCTGCTGCAAAAGCCATCTGTGACCACGTCAGGGACATCTGGTTTGGA
  ACCCCAGAGGGAGAGTTTGTGTCCATGGGTGTTATCTCTGATGGCAACTCCTATGGTGTT
  CCTGATGATCTGCTCTACTCATTCCCTGTTGTAATCAAGAATAAGACCTGGAAGTTTGTT
  GAAGGTCTCCCTATTAATGATTTCTCACGTGAGAAGATGGATCTTACTGCAAAGGAACTG
  ACAGAAGAAAAAGAAAGTGCTTTTGAATTTCTTTCCTCTGCCTGA
  
  Clone variation with respect to NM_005917.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005917 unedited
  TACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGCGGTAGAGGTGACCTGACTCTCT
  GAGGCTCATTTTGCAGTTGTTGAAATTGTCCCCGCAGTTTTCAATCATGTCTGAACCAAT
  CAGAGTCCTTGTGACTGGAGCAGCTGGTCAAATTGCATATTCACTGCTGTACAGTATTGG
  AAATGGATCTGTCTTTGGTAAAGATCAGCCTATAATTCTTGTGCTGTTGGATATCACCCC
  CATGATGGGTGTCCTGGACGGTGTCCTAATGGAACTGCAAGACTGTGCCCTTCCCCTCCT
  GAAAGATGTCATCGCAACAGATAAAGAAGACGTTGCCTTCAAAGACCTGGATGTGGCCAT
  TCTTGTGGGCTCCATGCCAAGAAGGGAAGGCATGGAGAGAAAAGATTTACTGAAAGCAAA
  TGTGAAAATCTTCAAATCCCAGGGTGCAGCCTTAGATAAATACGCCAAGAAGTCAGTTAA
  GGTTATTGTTGTGGGTAATCCAGCCAATACCAACTGCCTGACTGCTTCCAAGTCAGCTCC
  ATCCATCCCCAAGGAGAACTTCAGTTGCTTGACTCGTTTGGATCACAACCGAGCTAAAGC
  TCANATTGCTCTTAAACTTGGTGTGACTGCTAATGATGTAAAGAATGTCATTATCTGGNG
  AAACCATTCCTCGACTCAGTATCCAGATGTCAACCATGCCAAGGGTGAAATTGCAAGGAA
  AGGAAGNNTGGTGTTTATGAAAGCTCTGAAAGATGACAGCTGGCTCAAGGGAGATTTGTC
  ACGACTGTGCAGCAGCGTGGCGCTTCTGTCATCAAGGCTCGAAACTATCCATGCCATGTC
  TGCTGCAAAGCCATCTGTGACACGTCAGGGACATCTGGTTNGGGACCCAAGGAAGAGTTT
  GGTCATGGGTGTATCTCTGAGGCACTCCTAGGNGTCCTGAGAACTGCCTACTCATCCTGT
  GTATCAGATAGACCTGGAGTTGGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005917 unedited
  GCAACTTTCCAGGGCCAGGAATAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATC
  TGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTT
  TTCACTTTCATTTGAATATATATTTATTTAGAATGCACACTAACAGCATGACGATAAACT
  GTCATTCACAAACCTGTACCAAGAAGCACGTAATCTTTAAAATGTGTTGTTTTTCACAAG
  TAATTTAAGTATAGCATTATTATTATTTGGTACTTGAGTCAAAGACGACATTTAGATTCT
  TCAGCTTTGAAGCATTTAGTAACATCATTGTCTAGTCAGGCAGAGGAAAGAAATTCAAAA
  GCACTTTCTTTTTCTTCTGTCAGTTCCTTTGCAGTAAGATCCATCTTCTCACGTGAGAAA
  TCATTAATAGGGAGACCTTCAACAAACTTCCAGGTCTTATTCTTGATTACAACAGGGAAT
  GAGTAGAGCAGATCATCAGGAACACCATAGGAGTTGCCATCAGAGATAACACCCATGGAC
  ACAAACTCTCCCTCTGGGGTTCCAAACCAGATGTCCCTGACGTGGTCACAGATGGCTTTT
  GCAGCAGACATGGCACTGGATAGTTTTCGAGCCTTGATGACAGCAGCGCCACGCTGCTGC
  ACAGTCGTGACAAATTCTCCCTTGAGCCAGCTGTCATCTTTCAGAGCTTCATAACACCAA
  CTTCCTTTCCTTGCATTTTCACCTTGGCATGGTTGACATCTGGATACTGAGTCGAGAAAT
  GGTTTCCCCAGATATGACATTCTTTACATCATAACAGNCACACAAGTTTAGAGCTTTTGA
  GCTTACCTCGGTTGGGATCCAACGAGTCAGCAACTGAGTTCTCCTTGGGGATGAAGGAGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005917
• Insert Size: 1370 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005917.2, NP_005908.1
• RefSeq Size: 1268 bp
• RefSeq ORF: 1005 bp
• Locus ID: 4190
• UniProt ID: P40925
• Cytogenetics: 2p15
• Domains: ldh
• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Glyoxylate and dicarboxylate metabolism, Metabolic pathways, Pyruvate metabolism
• Gene Summary: This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6. [provided by RefSeq, Feb 2016]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (MDH1) results from translation termination at the upstream UGA stop codon, while the longer isoform (MDH1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (MDH1), which is localized in the cytosol.