Isocitrate dehydrogenase (IDH1) (NM_005896) Human Untagged Clone

CAT#: SC116430

IDH1 (untagged)-Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Isocitrate dehydrogenase
• Synonyms: HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116430 sequence for NM_005896 edited (data generated by NextGen Sequencing)
  ATGTCCAAAAAAATCAGTGGCGGTTCTGTGGTAGAGATGCAAGGAGATGAAATGACACGA
  ATCATTTGGGAATTGATTAAAGAGAAACTCATTTTTCCCTACGTGGAATTGGATCTACAT
  AGCTATGATTTAGGCATAGAGAATCGTGATGCCACCAACGACCAAGTCACCAAGGATGCT
  GCAGAAGCTATAAAGAAGCATAATGTTGGCGTCAAATGTGCCACTATCACTCCTGATGAG
  AAGAGGGTTGAGGAGTTCAAGTTGAAACAAATGTGGAAATCACCAAATGGCACCATACGA
  AATATTCTGGGTGGCACGGTCTTCAGAGAAGCCATTATCTGCAAAAATATCCCCCGGCTT
  GTGAGTGGATGGGTAAAACCTATCATCATAGGTCGTCATGCTTATGGGGATCAATACAGA
  GCAACTGATTTTGTTGTTCCTGGGCCTGGAAAAGTAGAGATAACCTACACACCAAGTGAC
  GGAACCCAAAAGGTGACATACCTGGTACATAACTTTGAAGAAGGTGGTGGTGTTGCCATG
  GGGATGTATAATCAAGATAAGTCAATTGAAGATTTTGCACACAGTTCCTTCCAAATGGCT
  CTGTCTAAGGGTTGGCCTTTGTATCTGAGCACCAAAAACACTATTCTGAAGAAATATGAT
  GGGCGTTTTAAAGACATCTTTCAGGAGATATATGACAAGCAGTACAAGTCCCAGTTTGAA
  GCTCAAAAGATCTGGTATGAGCATAGGCTCATCGACGACATGGTGGCCCAAGCTATGAAA
  TCAGAGGGAGGCTTCATCTGGGCCTGTAAAAACTATGATGGTGACGTGCAGTCGGACTCT
  GTGGCCCAAGGGTATGGCTCTCTCGGCATGATGACCAGCGTGCTGGTTTGTCCAGATGGC
  AAGACAGTAGAAGCAGAGGCTGCCCACGGGACTGTAACCCGTCACTACCGCATGTACCAG
  AAAGGACAGGAGACGTCCACCAATCCCATTGCTTCCATTTTTGCCTGGACCAGAGGGTTA
  GCCCACAGAGCAAAGCTTGATAACAATAAAGAGCTTGCCTTCTTTGCAAATGCTTTGGAA
  GAAGTCTCTATTGAGACAATTGAGGCTGGCTTCATGACCAAGGACTTGGCTGCTTGCATT
  AAAGGTTTACCCAATGTGCAACGTTCTGACTACTTGAATACATTTGAGTTCATGGATAAA
  CTTGGAGAAAACTTGAAGATCAAACTAGCTCAGGCCAAACTTTAA
  
  Clone variation with respect to NM_005896.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005896 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATCCCGGCAGGCGATA
  AACTACATTCAGTTGAGTCTGCAAGACTGGGAGGAACTGGGGTGATAAGAAATCTATTCA
  CTGTCAAGGTTTATTGAAGTCAAAATGTCCAAAAAAATCAGTGGCGGTTCTGTGGTAGAG
  ATGCAAGGAGATGAAATGACACGAATCATTTGGGAATTGATTAAAGAGAAACTCATTTTT
  CCCTACGTGGAATTGGATCTACATAGCTATGATTTAGGCATAGAGAATCGTGATGCCACC
  AACGACCAAGTCACCAAGGATGCTGCAGAAGCTATAAAGAAGCATAATGTTGGCGTCAAA
  TGTGCCACTATCACTCCTGATGAGAAGAGGGTTGAGGAGTTCAAGTTGAAACAAATGTGG
  AAATCACCAAATGGCACCATACGAAATATTCTGGGTGGCACGGTCTTCAGAGAAGCCATT
  ATCTGCAAAAATATCCCCCGGCTTGTGAGTGGATGGGTAAAACCTATCATCATAGGTCGT
  CATGCTTATGGGGATCAATACAGAGCAACTGATTTTGTTGTTCCTGNGCCTGGAAAAGTA
  GAGATAACCTACACACCAAGTGACGGAACCCAAAAGGTGACATACCTGGTACATAACTTT
  GAAGAAGGGTGGTGGTGTTGCCATGGNNGATGTATAATCAAGATAAGTCAATTGAAGATT
  TTGCACACAGTTCCTTCCAATGGCTCTGTCTAAGGGTTNGGCCTTTGTATCTGAGCCCAA
  AACACTATTCTGAGAATATGANTGGGCGTTTAAGACATCTTCAGAGATTATGACAGCAGT
  ACAGGTCCAGTTGAGCTCANNAGATCTGTATGACATAGCTCATCACN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005896 unedited
  CGCAATCTAGAGTCGAGTNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTAAAGGGGAATTACCCCTTATTTTTAATTAAAATCACCCGTTCAGGAAAAAATAAA
  ATAGGGTTAAATTTCAATTTCTGAAAAATCCCTTTTTTTTAAAAAAAATAAACCGGGCCC
  CCAAAAGTCCAAAAAAAATTCACCTTACTTTTTTGCCCTTGGATAAAATTTCCTTTTTAA
  AGTATTTTAATTTTCAGGCCCGCCCCGGAGGAAAAAAAAAAAAATGGGGAGGACAACCCT
  CCAGGTAGTTTTTTTTCCGGATTCCAAACTTTCCTGGGGCCTAAACAGTTTTTATTCTTT
  TGGAAACATTCACCAGGGTTTTTACAAAAATGACTGCAGTTTCTTCAACCCATTTGAGTG
  GCACTCATCCTTCGTTCCAGTCTTGTGCAATTTTAATGCCCACTTCTTCCTTAAAAAACG
  GATTTTTTTTACCCCAAATTTCCCTGTCCCCAAGTTTTGGCCAGGAGGGGAAGCCCGGAA
  ATTCCCTTCTTCGCTTTTTAGGGGACATTCCGGGGGTTTTTTTCCCCTTTAGAACCCTTG
  TTTTTGGCCTTTTTTATTAAACCCGGAAAAAAGGGCCGGGATCCCAGTGTTCCTCCCCGA
  TTTCTTGGGGGGGCCCAAAAAAAAAAAAAAATTTTAAAAACCCCCTTCCTTTTTCCCAAA
  GGGGGCGATACGTTTTTTAAAAAAAAGGGGGGGACCTTTAAAAAAAAAAACTTGGGTTTT
  TAAAAAAAAAAAAAAAAGGGTCCCCTTTTCCCTCGGGGGCAACAACAAAAAGAAACCCTG
  CCCACCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005896
• Insert Size: 2210 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005896.2, NP_005887.2
• RefSeq Size: 2339 bp
• RefSeq ORF: 1245 bp
• Locus ID: 3417
• UniProt ID: O75874
• Cytogenetics: 2q34
• Domains: isodh
• Protein Pathways: Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways
• Gene Summary: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]
  Transcript Variant: This variant (1) and variants 2 and 3 encode the same protein.