ACADM (NM_000016) Human Untagged Clone

CAT#: SC116219

ACADM (untagged)-Human acyl-CoA dehydrogenase, C-4 to C-12 straight chain (ACADM), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACADM
• Synonyms: ACAD1; MCAD; MCADH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116219 sequence for NM_000016 edited (data generated by NextGen Sequencing)
  ATGGCAGCGGGGTTCGGGCGATGCTGCAGGGTCCTGAGAAGTATTTCTCGTTTTCATTGG
  AGATCACAGCATACAAAAGCCAATCGACAACGTGAACCAGGATTAGGATTTAGTTTTGAG
  TTCACCGAACAGCAGAAAGAATTTCAAGCTACTGCTCGTAAATTTGCCAGAGAGGAAATC
  ATCCCAGTGGCTGCAGAATATGATAAAACTGGTGAATATCCAGTCCCCCTAATTAGAAGA
  GCCTGGGAACTTGGTTTAATGAACACACACATTCCAGAGAACTGTGGAGGTCTTGGACTT
  GGAACTTTTGATGCTTGTTTAATTAGTGAAGAATTGGCTTATGGATGTACAGGGGTTCAG
  ACTGCTATTGAAGGAAATTCTTTGGGGCAAATGCCTATTATTATTGCTGGAAATGATCAA
  CAAAAGAAGAAGTATTTGGGGAGAATGACTGAGGAGCCATTGATGTGTGCTTATTGTGTA
  ACAGAACCTGGAGCAGGCTCTGATGTAGCTGGTATAAAGACCAAAGCAGAAAAGAAAGGA
  GATGAGTATATTATTAATGGTCAGAAGATGTGGATAACCAACGGAGGAAAAGCTAATTGG
  TATTTTTTATTGGCACGTTCTGATCCAGATCCTAAAGCTCCTGCTAATAAAGCCTTTACT
  GGATTCATTGTGGAAGCAGATACCCCAGGAATTCAGATTGGGAGAAAGGAATTAAACATG
  GGCCAGCGATGTTCAGATACTAGAGGAATTGTCTTCGAAGATGTGAAAGTGCCTAAAGAA
  AATGTTTTAATTGGTGACGGAGCTGGTTTCAAAGTTGCAATGGGAGCTTTTGATAAAACC
  AGACCTGTAGTAGCTGCTGGTGCTGTTGGATTAGCACAAAGAGCTTTGGATGAAGCTACC
  AAGTATGCCCTGGAAAGGAAAACTTTCGGAAAGCTACTTGTAGAGCACCAAGCAATATCA
  TTTATGCTGGCTGAAATGGCAATGAAAGTTGAACTAGCTAGAATGAGTTACCAGAGAGCA
  GCTTGGGAGGTTGATTCTGGTCGTCGAAATACCTATTATGCTTCTATTGCAAAGGCATTT
  GCTGGAGATATTGCAAATCAGTTAGCTACTGATGCTGTGCAGATACTTGGAGGCAATGGA
  TTTAATACAGAATATCCTGTAGAAAAACTAATGAGGGATGCCAAAATCTATCAGATTTAT
  GAAGGTACTTCACAAATTCAAAGACTTATTGTAGCCCGTGAACACATTGACAAGTACAAA
  AATTAA
  
  Clone variation with respect to NM_000016.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000016 unedited
  TTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGTGCCACCCCGCCTAGCG
  CAGCGCCCCGTCCTTCCGCAGCCCAACCGCCTCTTCCCGCCCCGCCCCATCCCGCCCACG
  GGCTCCAGTGGGCGGGACCAGAGGAGTCCCGCGTTCGGGGAGTATGTCAAGGCCGTGACC
  CGTGTATTATTGTCCGAGTGGCCGGAACGGGAGCCAACATGGCAGCGGGGTTCGGGCGAT
  GCTGCAGGGTCCTGAGAAGTATTTCTCGTTTTCATTGGAGATCACAGCATACAAAAGCCA
  ATCGACAACGTGAACCAGGATTAGGATTTAGTTTTGAGTTCACCGAACAGCAGAAAGAAT
  TTCAAGCTACTGCTCGTAAATTTGCCAGAGAGGAAATCATCCCAGTGGCTGCAGAATATG
  ATAAAACTGGTGAATATCCAGTCCCCCTAATTAGAAGAGCCTGGGAACTTGGTTTAATGA
  ACACACACATTCCAGAGAACTGTGGAGGTCTTGGACTTGGAACTTTTGATGCTTGTTTAA
  TTAGTGAAGAATTGGCTTATGGATGTACAGGGGTTCAGACTGCTATTGAAGGAAATTCTT
  TGGGGCAAATGCCTATTATTATTGCTGGAAATGATCAACAAAAGAAGAAGTATTTGGGGA
  GAATGACTGANGAGCCATTGATGTGTGCTTATTGTGTAACAGAACCTGGAGCANGCTCTG
  ATGTAGCTGGGTATAAGACCANAGCAGAAAAGAAAGGAGATGAGTATATTATTAATGGTC
  AGAAGATGTGGATTACCAACGGNAGGAAAAGCTATTGGTATTTTTATTGGCACGTTCTGA
  TCCAGATCCTAAAGCTNCTGCTAATAAAGCCTTTACTGGATTCATTGGGGAAGCAGATAC
  CCANG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000016 unedited
  CTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTAAGCACTGA
  ACAATTTTATTAAGTTCTTTGCCAAAATGCAAACATATGAAATGAGATTGTGTACAATAG
  ACCAAAGGCTACCTAGAGACCAACAGTCATATAAAAATAATTTAAGGCAAGCTTTATTGA
  TTTATTTTAAATAAGGTAAGTGAAATATGGGCTCAGAATTAAATAAGATTTTCAAGAAAG
  TTCTTGTAGCTGTAAAATAGGAGAATATGAATGTCAAAATTTTGACTGTTTCTGTGCCAA
  ACAGAAAGTTTCACAAATGCTTTCCAAACTGCAATAAAAAGAACTTTAATAATACCATTA
  AGTCTACAAAGTTACTGTCAAGTTGACAGTATTCAAATACTACAATATTTTCTAAATGTA
  AATTTTGTAACATCTTGAATACTTTCTAAGCCCACTTTTCTGTCATTACAAAAATAAAAT
  TCCAAGAAATTTCTGCTACATTAATCTTAAGAGATCAAAATAACCTATGTAGTTTTCTAG
  GTTAATGTAATTCAAGTAAAGTGGTACTAAAGAAAACACATCATTTTAATAAAAACCAAA
  CCTGCTTAAGAGACATAACAGAGTTAAGCAAAAGTATAAACTACTATAATAAGCAGTTGC
  TTAGATTTAATATAAGAGGATTTGTTTTCACTGGAAAAAACGTTAAAGCCCTTTCTTTTT
  TCTGGAGCTGAAACAGTGGCTTGTGTTCTAGTTATTCAATATTTCTACAGNAATTTTTTT
  TATTTTTTGCACTTGNNCAAGGCCGTCACGGGCCTACAATAGTCTTTGAATTTGTGAAGT
  ACCTTCATAATCTGATAGATTTGGCATCCCTCATTAGTTTTCTACAGGATATTCTGTATA
  AATCCATTGCCTCCAGTTTCTGCCAGGATCAGTAGCTACTGATCTGAATATCTCCANCAA
  GCCTTTGAATAGAGCCTATAATATTTCGACACACAATANCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000016
• Insert Size: 2190 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000016.2, NP_000007.1
• RefSeq Size: 2192 bp
• RefSeq ORF: 1266 bp
• Locus ID: 34
• UniProt ID: P11310
• Cytogenetics: 1p31.1
• Domains: Acyl-CoA_dh, Acyl-CoA_dh_M, Acyl-CoA_dh_N
• Protein Families: Druggable Genome
• Protein Pathways: beta-Alanine metabolism, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway, Propanoate metabolism, Valine, leucine and isoleucine degradation
• Gene Summary: This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.