Complement factor 8 beta (C8B) (NM_000066) Human Untagged Clone

CAT#: SC115997

C8B (untagged)-Human complement component 8, beta polypeptide (C8B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Complement factor 8 beta
• Synonyms: C82
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115997 sequence for NM_000066 edited (data generated by NextGen Sequencing)
  ATGAAGAATTCCAGGACATGGGCTTGGAGGGCGCCGGTGGAGCTATTTCTTCTCTGTGCT
  GCCCTGGGCTGTCTCAGTTTGCCTGGCTCCAGAGGTGAAAGGCCACATTCCTTTGGGTCA
  AATGCAGTCAACAAGAGCTTTGCTAAGAGCAGACAGATGCGGAGTGTGGATGTTACCCTG
  ATGCCCATTGATTGTGAGCTGTCTAGTTGGTCCTCTTGGACCACATGTGACCCCTGTCAG
  AAGAAAAGGTACAGGTATGCCTACTTGCTCCAGCCCTCTCAGTTCCATGGGGAACCGTGC
  AACTTCTCTGACAAGGAAGTCGAAGACTGTGTTACCAACAGACCATGCAGAAGTCAAGTG
  CGATGTGAAGGCTTTGTGTGTGCACAGACAGGAAGGTGTGTAAACCGCAGACTTCTTTGC
  AATGGGGACAATGACTGTGGAGACCAGTCAGATGAAGCAAACTGTAGAAGGATTTATAAA
  AAATGTCAGCATGAAATGGACCAATACTGGGGAATTGGCAGTCTGGCCAGTGGGATAAAT
  TTGTTCACAAACAGTTTTGAGGGCCCAGTTCTTGATCACAGGTATTATGCAGGTGGATGC
  TCCCCGCATTACATCCTGAACACGAGGTTTAGGAAGCCCTACAATGTGGAAAGCTACACG
  CCACAGACCCAAGGCAAATACGAATTCATATTAAAAGAGTATGAATCATACTCAGATTTT
  GAACGCAATGTCACAGAGAAAATGGCAAGCAAGTCTGGTTTCAGTTTTGGTTTTAAAATA
  CCTGGAATATTTGAACTTGGCATCAGTAGTCAAAGTGATCGAGGCAAACACTATATTAGG
  AGAACCAAACGATTCTCTCATACTAAAAGCGTATTTCTGCATGCACGCTCTGACCTTGAA
  GTAGCACATTACAAGCTGAAACCCAGAAGCCTCATGCTCCATTACGAGTTCCTTCAGAGA
  GTTAAGCGGCTGCCCCTGGAGTACAGCTACGGGGAATACAGAGATCTCTTCCGTGATTTT
  GGGACCCACTACATCACAGAGGCTGTGCTTGGGGGCATTTATGAATACACCCTCGTTATG
  AACAAAGAGGCCATGGAGAGAGGAGATTATACTCTTAACAACGTCCATGCCTGTGCCAAA
  AATGATTTTAAAATTGGTGGTGCCATTGAAGAGGTCTACGTCAGTCTGGGTGTGTCTGTA
  GGCAAATGCAGAGGTATTCTGAATGAAATAAAAGACAGAAACAAGAGGGACACCATGGTG
  GAGGACTTGGTGGTCCTGGTACGAGGAGGGGCAAGTGAGCACATCACCACCCTGGCATAC
  CAGGAGCTGCCGACGGCGGACCTGATGCAGGAGTGGGGAGACGCTGTGCAGTACAACCCA
  GCCATCATCAAAGTTAAGGTGGAGCCTCTGTATGAACTAGTGACAGCCACAGATTTTGCC
  TATTCCAGCACAGTGAGGCAGAACATGAAGCAGGCACTGGAGGAGTTCCAGAAGGAAGTT
  AGTTCCTGCCACTGTGCTCCCTGCCAAGGAAATGGAGTCCCTGTCCTGAAAGGATCACGC
  TGTGACTGCATCTGTCCTGTTGGATCCCAAGGCCTAGCCTGTGAGGTCTCCTATCGGAAG
  AATACCCCCATTGATGGGAAGTGGAATTGCTGGTCAAATTGGTCTTCATGCTCTGGAAGA
  CGTAAGACAAGACAAAGGCAGTGTAACAATCCACCTCCTCAAAATGGGGGTAGCCCCTGT
  TCAGGCCCTGCTTCAGAAACACTTGACTGCTCCTAG
  
  Clone variation with respect to NM_000066.2
  349 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000066 unedited
  NNNNNGGGTTCAGATTTGTATACGACTCCTATAGGCGGCCGCGGTTTACTTTGCAGAGCT
  ATTTGCTCTTGGGGACAGAAGCTGACAGTGGCACTCACAGCACAGGCTTGTTATGGGTCT
  AGCAGCCTCTGTGGCATCTCCTGTCACATTGGGAAAATGAAGAATTCCAGGACATGGGCT
  TGGAGGGCGCCGGTGGAGCTATTTCTTCTCTGTGCTGCCCTGGGCTGTCTCAGTTTGCCT
  GGCTCCAGAGGTGAAAGGCCACATTCCTTTGGGTCAAATGCAGTCAACAAGAGCTTTGCT
  AAGAGCAGACAGATGCGGAGTGTGGATGTTACCCTGATGCCCATTGATTGTGAGCTGTCT
  AGTTGGTCCTCTTGGACCACATGTGACCCCTGTCAGAAGAAAAGGTACAGGTATGCCTAC
  TTGCTCCAGCCCTCTCAGTTCCATGGGGAACCGTGCAACTTCTCTGACAAGGAAGTCGAA
  GACTGTGTTACCAACAGACCATGCAGAAGTCAAGTGCGATGTGAAGGCTNTGTGTGTGCA
  CAGACAGGAAGGTGTGTAAACCGCAGACTTCTTTGNCATGGGGACAATGACTGTGGAGAC
  CAGTCAGATGAAGCAAACTGTAGAAGGATTTATAAAAAATGTCAGCATGAAATGGACCAA
  TACTGNGGAAATTGGCAGTCTGGCCAGTGGGATAAATTTGTTACAAACAGTTTTGAGGGC
  CCCAGTCTTGATCACAGGTATTATNGCAGGNTGGATGCTCCCCGCATTACATNCTGACAA
  CGAGGNTTTANGAAGCCCTACNNATGTGAAAGCTACACGCCACAGACCCCAGGCAATACG
  AATTCTATTAAAAGAGTNTGAATCTACTCAGATTTGAACGCATGTCCANAGAATGGCAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000066 unedited
  NTTTTTGACTTGNACNCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTTTT
  TAAGCCTTTAACTCAGTATTTATTTAAATCAACTTGCATTTTACAAGGTAACATCTTTAT
  TTTAAACAGCTTGCATGGCACTGCCTTTTGCCCTTGCATGAACTCCAGGTGGAAACTGGT
  GTAGGGCTGAGCTGGCATGAGTTCTTGAGGGCTCAGGGCTCTCATTGTATGTAGCCCACT
  GCTGTATCATCTGCTAGGAGCAGTCAAGTGTTTCTGAAGCAGGGCCTGAACAGGGGCTAC
  CCCCATTTTGAGGAGGTGGATTGTTACACTGCCTTTGTCTTGTCTTACGTCTTCCAGAGC
  ATGAAGACCAATTTGACCAGCAATTCCACTTCCCATCAATGGGGGTATTCTTCCGATAGG
  AGACCTCACAGGCTAGGCCTTGGGATCCAACAGGACAGATGCAGTCACAGCGTGATCCTT
  TCAGGACAGGGACTCCATTTCCTTGGCAGGGAGCACAGTGGCAGGAACTAACTTCCTTCT
  GGAACTCCTCCAGTGCCTGCTTCATGTTCTGCCTCACTGTGCTGGAATAGGCAAAATCTG
  TGGCTGTCACTAGTTCATACAGAGGCTCCACCTTAACTTTGATGATGGCTGGGGTTGTAC
  TGCACAGCGTCTCCCCACTCCTGCATCAGGTCCGCCGTCGGCAGCTCCTGGTATGCCAGG
  GGTGGTGATGTGCTCACTTGCCCCTCCTCGTACAGGACCACCAAGTCCTCCCCATGGNGN
  NCCTCNTGNTTCTGGCTTTTATTTCATTCAGAATACCTCTGCATTTGCCTACAGACACCC
  CAGACTGACGTAGACCTCTTCATGGCACCACCCATTTAAAANTCATTTTGGCACNNNGCA
  TGAACGTGTAAAAGNATATCTCCTCTCTCATGCCCCTCTTGTATAACCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000066
• Insert Size: 2150 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000066.2, NP_000057.1
• RefSeq Size: 2053 bp
• RefSeq ORF: 1776 bp
• Locus ID: 732
• UniProt ID: P07358
• Cytogenetics: 1p32.2
• Domains: tsp_1, MACPF, ldl_recept_a
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus
• Gene Summary: This gene encodes one of the three subunits of the complement component 8 (C8) protein. C8 is composed of equimolar amounts of alpha, beta and gamma subunits, which are encoded by three separate genes. C8 is one component of the membrane attack complex, which mediates cell lysis, and it initiates membrane penetration of the complex. This protein mediates the interaction of C8 with the C5b-7 membrane attack complex precursor. In humans deficiency of this protein is associated with increased risk of meningococcal infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
  Transcript Variant: This variant (1) encodes the longest isoform (1).