POLR3A (NM_007055) Human Untagged Clone

CAT#: SC115726

POLR3A (untagged)-Human polymerase (RNA) III (DNA directed) polypeptide A, 155kDa (POLR3A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR3A
• Synonyms: ADDH; C160; HLD7; hRPC155; RPC1; RPC155; WDRTS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115726 sequence for NM_007055 edited (data generated by NextGen Sequencing)
  ATGGTGAAGGAGCAGTTCCGGGAGACGGATGTGGCCAAGAAAATAAGCCACATCTGTTTT
  GGAATGAAGTCACCTGAGGAGATGCGCCAGCAGGCGCACATCCAAGTTGTGAGTAAGAAC
  CTGTACAGCCAGGACAACCAACATGCCCCCTTGCTATATGGGGTGCTCGACCATAGGATG
  GGTACGAGTGAGAAGGATCGTCCATGTGAAACCTGTGGGAAAAACTTGGCTGACTGTCTA
  GGCCACTATGGGTATATCGACCTGGAGTTGCCGTGTTTTCATGTAGGGTACTTCAGAGCA
  GTCATAGGCATCTTACAGATGATCTGCAAAACCTGCTGCCACATCATGCTGTCCCAAGAG
  GAGAAGAAGCAGTTTCTGGACTATCTAAAGAGGCCCGGCCTGACCTACCTTCAGAAGCGA
  GGACTGAAAAAGAAAATCTCTGACAAGTGCCGGAAGAAAAACATCTGCCATCACTGTGGC
  GCTTTTAATGGTACCGTAAAGAAGTGTGGACTGCTGAAAATAATTCATGAGAAATACAAG
  ACCAACAAAAAAGTGGTGGATCCCATTGTATCAAATTTCCTTCAGTCTTTTGAAACAGCC
  ATTGAACATAATAAAGAAGTGGAGCCTCTGCTGGGAAGGGCACAGGAAAACTTGAATCCC
  TTAGTAGTTCTGAATTTATTTAAACGAATCCCAGCTGAAGATGTTCCTCTACTTCTGATG
  AACCCAGAAGCCGGAAAGCCGTCTGATTTGATTCTCACACGACTTTTGGTGCCTCCTTTG
  TGTATCAGACCCTCCGTTGTGAGTGATTTGAAGTCTGGCACCAATGAAGATGATCTGACA
  ATGAAACTGACAGAAATCATTTTCCTAAACGATGTTATTAAAAAGCATCGGATCTCAGGA
  GCCAAGACCCAGATGATCATGGAGGACTGGGATTTCCTGCAGCTGCAGTGTGCCCTCTAC
  ATTAACAGTGAGCTCTCGGGCATTCCCCTCAACATGGCACCCAAGAAGTGGACCAGAGGC
  TTCGTCCAACGCCTGAAGGGAAAACAGGGTCGATTTAGAGGAAATCTCTCAGGAAAGAGA
  GTGGATTTTTCTGGCAGAACAGTCATCTCGCCCGACCCCAACCTCCGGATTGATGAGGTA
  GCTGTGCCAGTTCATGTGGCCAAAATTCTAACTTTTCCTGAGAAGGTAAACAAAGCAAAC
  ATCAATTTCTTGAGGAAACTGGTTCAAAACGGCCCTGAGGTTCACCCAGGAGCAAACTTC
  ATTCAGCAGAGACATACGCAGATGAAAAGGTTTTTGAAATACGGAAATCGAGAAAAGATG
  GCTCAAGAGCTCAAGTATGGTGACATCGTAGAGAGACACCTCATCGATGGAGATGTGGTG
  CTGTTCAATCGGCAGCCCTCGCTGCACAAATTGAGCATTATGGCTCATCTGGCCAGGGTC
  AAGCCCCACCGGACCTTCAGATTTAATGAGTGTGTCTGTACACCCTATAATGCTGACTTT
  GATGGTGATGAAATGAACCTTCATCTTCCTCAAACAGAAGAAGCTAAAGCAGAGGCCCTT
  GTTCTGATGGGGACTAAAGCAAATCTTGTAACCCCGAGGAATGGGGAACCGCTGATTGCT
  GCTATTCAGGATTTTCTAACAGGTGCCTATCTCCTCACTCTCAAGGACACTTTCTTTGAT
  CGAGCCAAGGCTTGCCAAATCATTGCTTCAATACTGGTTGGCAAGGATGAGAAAATTAAA
  GTTCGCCTCCCACCGCCTACAATCCTAAAGCCTGTCACCCTGTGGACGGGAAAGCAGATC
  TTCAGTGTCATCCTCAGGCCTAGCGATGACAATCCAGTGAGGGCCAACCTGCGAACCAAG
  GGCAAGCAGTACTGTGGCAAAGGGGAAGATCTCTGTGCCAATGATTCCTATGTTACAATC
  CAGAACAGTGAGTTGATGAGTGGCAGCATGGACAAAGGAACCCTAGGGTCAGGATCCAAG
  AACAATATTTTTTACATTTTGCTGCGAGACTGGGGACAGAATGAAGCTGCAGATGCCATG
  TCACGGCTCGCCAGGCTGGCTCCTGTCTACCTGTCTAACCGTGGTTTCTCAATTGGGATC
  GGTGATGTCACACCTGGCCAAGGACTGCTGAAGGCCAAGTATGAGTTGCTGAATGCCGGC
  TACAAGAAATGTGATGAGTACATCGAAGCCCTGAACACGGGCAAGCTGCAGCAGCAGCCT
  GGCTGCACTGCTGAGGAGACCCTGGAGGCACTGATCCTGAAGGAGCTGTCTGTGATCCGT
  GACCACGCTGGCAGTGCCTGCCTCCGGGAGCTGGACAAGAGCAACAGCCCCCTCACCATG
  GCTCTGTGCGGCTCCAAAGGTTCCTTCATTAACATATCACAGATGATTGCCTGTGTGGGA
  CAGCAGGCCATCAGTGGCTCTCGAGTGCCAGACGGCTTTGAAAACAGGTCCTTGCCTCAT
  TTTGAAAAACACTCAAAGCTCCCAGCTGCCAAAGGCTTTGTGGCTAATAGCTTTTATTCC
  GGTTTGACACCAACTGAGTTTTTCTTCCACACAATGGCCGGCCGGGAAGGTCTAGTCGAC
  ACGGCTGTAAAGACAGCTGAAACGGGATACATGCAGCGAAGGCTTGTCAAATCTCTTGAA
  GATCTTTGCTCCCAGTATGATCTGACAGTCCGAAGCTCTACTGGCGATATTATCCAGTTC
  ATTTATGGAGGAGATGGCTTAGATCCTGCAGCTATGGAGGGAAAAGATGAACCTTTGGAG
  TTTAAAAGGGTTCTGGACAACATCAAAGCAGTCTTCCCGTGTCCCAGTGAGCCTGCTCTC
  AGCAAAAACGAGCTGATCCTGACCACAGAGTCCATCATGAAGAAGAGTGAGTTCCTCTGC
  TGCCAGGACAGCTTCCTGCAGGAAATAAAAAAATTCATTAAGGGGGTCTCTGAGAAGATC
  AAGAAAACCAGAGATAAATATGGCATCAATGATAACGGCACAACAGAGCCCCGTGTGCTG
  TACCAGCTGGACCGCATCACCCCCACCCAAGTAGAAAAGTTTCTGGAGACCTGTAGGGAC
  AAGTACATGAGGGCACAGATGGAGCCAGGTTCTGCAGTGGGTGCTCTGTGTGCCCAGAGC
  ATTGGTGAGCCAGGCACCCAGATGACCCTGAAGACTTTCCACTTTGCAGGTGTGGCCTCC
  ATGAACATCACCCTGGGCGTGCCCCGGATTAAAGAGATCATCAACGCTTCCAAGGCCATC
  AGCACTCCAATTATCACAGCACAGCTAGACAAGGATGACGACGCGGATTATGCTCGCCTC
  GTGAAAGGGAGAATTGAGAAAACCCTCTTGGGAGAGATTTCCGAGTATATTGAAGAAGTG
  TTTCTTCCTGATGACTGCTTTATTCTCGTCAAGCTCTCCCTGGAACGGATTAGGCTTCTG
  AGACTGGAAGTGAACGCTGAGACAGTGAGATATTCCATCTGCACATCCAAGCTCCGTGTG
  AAGCCCGGTGATGTGGCTGTTCATGGTGAGGCTGTGGTGTGTGTCACCCCCAGAGAGAAC
  AGCAAGAGCTCCATGTACTACGTGCTGCAGTTCCTGAAAGAGGATCTCCCCAAGGTGGTG
  GTGCAGGGCATTCCAGAGGTGTCCAGAGCTGTCATCCACATTGACGAGCAGAGTGGAAAG
  GAGAAGTACAAGCTTCTGGTGGAAGGTGATAACCTGCGGGCAGTCATGGCCACACACGGT
  GTGAAGGGCACCCGAACCACCTCCAATAACACCTATGAGGTGGAGAAAACTCTGGGCATC
  GAGGCCGCCCGGACAACGATCATCAATGAAATCCAGTACACCATGGTGAACCACGGCATG
  AGCATCGACAGGAGGCACGTGATGCTGCTCTCCGACCTCATGACCTACAAGGGTGAAGTC
  CTGGGCATCACTAGGTTTGGCCTGGCCAAGATGAAGGAGAGTGTGCTGATGCTGGCCTCC
  TTTGAGAAGACGGCTGACCATCTCTTTGACGCTGCCTACTTCGGGCAGAAGGACTCTGTG
  TGTGGGGTGTCTGAGTGCATCATCATGGGAATCCCAATGAACATTGGAACCGGGCTCTTC
  AAGCTGCTTCACAAGGCTGACAGGGACCCGAACCCTCCCAAGAGGCCCCTGATCTTCGAC
  ACAAATGAATTCCACATCCCCCTTGTCACATAG
  
  Clone variation with respect to NM_007055.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007055 unedited
  TCCTATAGGGCGGCCGCGAATTCGCACGAGGCCAGGAGGTGCTGGGGGCATCGTTTCTCT
  AATCTGGCCTCCCGAGTGCCAAGGAGGCGTCCCGGCAGCGGTCATCATGGTGAAGGAGCA
  GTTCCGGGAGACGGATGTGGCCAAGAAAATAAGCCACATCTGTTTTGGAATGAAGTCACC
  TGAGGAGATGCGCCAGCAGGCGCACATCCAAGTTGTGAGTAAGAACCTGTACAGCCAGGA
  CAACCAACATGCCCCCTTGCTATATGGGGTGCTCGACCATAGGATGGGTACGAGTGAGAA
  GGATCGTCCATGTGAAACCTGTGGGAAAAACTTGGCTGACTGTCTAGGCCACTATGGGTA
  TATCGACCTGGAGTTGCCGTGTTTTCATGTAGGGTACTTCAGAGCAGTCATAGGCATCTT
  ACAGATGATCTGCAAAACCTGCTGCCACATCATGCTGTCCCAAGAGGAGAAGAAGCAGTT
  TCTGGACTATCTAAAGAGGCCCGGCCTGACCTACCTTCAGAAGCGAGGACTGAAAAAGAA
  AATCTCTGACAAGTGCCGGAAGAAAAACATCTGCCATCACTGTGGCGCTTTTAATGGTAC
  CGTAAAGAAGTGTGGACTGCTGAAAATAATTCATGAGAAATACAAGACCAACAAAAAAGT
  GGTGGATCCCATTGTATCAAATTTCCTTCAGTCTTTTGAAACAGCCATTGAACATAATAA
  AGAAGTGGAGCCTCTGCTGGGAAGGGCACAGGAAAAACTTGATCCCTTAGTAGTTCTGAA
  TTTATTTAAACGAATCCCAGCTGAAAGATGTCCTCTACTTCTGATGAACCCAGAGCCGNA
  AAGCCGTCTGATTTGATTCTCACACGACTTTTNTGGCTNCCTTGTGTATAGACCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007055 unedited
  GGCGTAAGATAAACTTNCTATCAAAAAACCANCTCATCCCCATTGCAGCAGGCCCCAACT
  CCGTGAGGCTTCCATAGGCCATGGAAAGGGTTTGGCTCTTGTCACAGGGTAGAGCCCCAT
  GATAGGGGTCTGAGGTAGATGAAAACAACAGGGTAGCCAGGGGCATCTATAGAAGATGTT
  ACAGAGCTGCCTGGGCCTCATGACAGTTGCACCTCAGTTACAAATCCCAGACGGCTCTAT
  TTGCTTAAACCAGTTTTTCCAGTGACGCTTACATGCTGACTTTCTTGGTTTCTATAGATG
  CTTGGTTTCAAAAATAAAACTAAAATAACAAAAATTCCAAATCCCTCAAATGGAACAAAT
  AAATAATAGTAACAATATTTACATAAGTATGTCACATTGAGAGATTCCAGCATGACCAAG
  GAATGGAAAATCCAAGAATGTGCCCTGGCCTGTGTGGGGCTCAGGCCTGGCTCATCGTCA
  GGTGTGAAGACACCATGGGGAGCCGATGGATGTATGCAGTGAGCTGGGATGGACCATGAC
  ACCTGGCTGGGCTTTAAGTCCAGGGAAGCAGTGTGTGCACGAGCTGTGGGGCCGTCTATA
  GATTAGGTTTCAAGCAAGCAAACAATAAAACCCTCAGCTCTCCCGAGCAGCGTGGCACAG
  TCAGGGTCACTGGTGTGAGCTGCACCCTATCAGAGGAGAAGCTGCTCCTGGGGATGCCAA
  GAGGGCTTCTCTGATTGCTGGCATANGTGGGGACCTCAGGACCCCCGTTCCCAGGAGCAC
  AAAACTCTTTATACATCAGCTGGAGACACGGACAGGAGTCAAGGTCAGGCATGGTCCCCT
  CTTTCTTTGGACTATGTGACAGGGNGGATGTGGAATTCATTTGTGTCGCAGATCAGGGGC
  CTCTGGGGAGGGTCGGNTCCCTGTCAGCCTTGGAAGCACCTGGAGAGCCCGGTCTCATGT
  TCATTGGGATTCCATGAGATGCACTCAGCACCCACACACAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007055
• Insert Size: 5270 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007055.2, NP_008986.2
• RefSeq Size: 5618 bp
• RefSeq ORF: 4173 bp
• Locus ID: 11128
• UniProt ID: O14802
• Cytogenetics: 10q22.3
• Domains: RNA_pol_Rpb1_2, RPOLA_N, RNA_pol_Rpb1_3, RNA_pol_Rpb1_1, RNA_pol_Rpb1_5, RNA_pol_Rpb1_4
• Protein Families: Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: The protein encoded by this gene is the catalytic component of RNA polymerase III, which synthesizes small RNAs. The encoded protein also acts as a sensor to detect foreign DNA and trigger an innate immune response. [provided by RefSeq, Aug 2011]