PYCR2 (NM_013328) Human Untagged Clone

CAT#: SC115284

PYCR2 (untagged)-Human pyrroline-5-carboxylate reductase family, member 2 (PYCR2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PYCR2
• Synonyms: HLD10; P5CR2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115284 sequence for NM_013328 edited (data generated by NextGen Sequencing)
  ATGAGCGTGGGCTTCATCGGGGCCGGCCAGCTGGCCTATGCTCTGGCGCGGGGCTTCACG
  GCCGCAGGCATCCTGTCGGCTCACAAGATAATAGCCAGCTCCCCAGAAATGAACCTGCCC
  ACGGTGTCCGCGCTCAGGAAGATGGGTGTGAACCTGACACGCAGCAACAAGGAGACGGTG
  AAGCACAGCGACGTCCTGTTTCTGGCTGTGAAGCCACATATCATCCCCTTCATCCTGGAT
  GAGATTGGGGCCGACGTGCAAGCCAGACACATCGTGGTCTCCTGTGCGGCTGGTGTCACC
  ATCAGCTCTGTGGAGAAGAAGCTGATGGCATTCCAGCCAGCCCCCAAAGTGATTCGCTGC
  ATGACCAACACACCTGTGGTAGTGCAGGAAGGCGCTACAGTGTACGCCACGGGCACCCAT
  GCCCTGGTGGAGGATGGGCAGCTCCTGGAGCAGCTCATGAGCAGCGTGGGCTTCTGCACT
  GAGGTGGAAGAGGACCTCATCGATGCCGTCACGGGGCTCAGTGGCAGCGGGCCTGCCTAT
  GCATTCATGGCTCTGGACGCATTGGCTGATGGTGGGGTGAAGATGGGTTTGCCACGGCGC
  CTGGCAATCCAACTCGGGGCCCAGGCTTTGCTGGGAGCTGCCAAGATGCTGCTGGACTCG
  GAGCAGCATCCATGCCAGCTTAAGGACAATGTCTGCTCCCCTGGGGGAGCCACCATCCAC
  GCCCTGCACTTTCTAGAGAGTGGGGGCTTCCGCTCTCTGCTCATCAATGCAGTTGAGGCC
  TCCTGTATCCGAACACGAGAGCTACAGTCCATGGCCGACCAAGAAAAGATCTCCCCAGCT
  GCCCTTAAGAAGACCCTCTTAGACAGAGTGAAGCTGGAATCCCCCACAGTCTCCACACTG
  ACCCCCTCCAGCCCAGGGAAGCTCCTCACAAGAAGCCTGGCCCTGGGAGGCAAGAAGGAC
  TAA
  
  Clone variation with respect to NM_013328.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013328 unedited
  NTGTCAGAATTTTGGTATACGACTCACTTATAGGGCGGCCGCGAATCGGCACCAGGCCGC
  CCGGGAGATATCCGCCGGGGGAGAATAGGGTTGCACCATCCCAGAAGCTGCCGTTAGCTC
  GCCGGTCCTCGGCACGCCGCCCGTTCGCCCCTGCGCTGTCCGCCTTTCCCCTAGCGTTAC
  TTCCGGTCCCTCGCTGAGGGGGTTCCGTGCGGCTCCCAGGAGGCGTGAACCGCGGACCAT
  GAGCGTGGGCTTCATCGGGGCCGGCCAGCTGGCCTATGCTCTGGCGCGGGGCTTCACGGC
  CGCAGGCATCCTGTCGGCTCACAAGATAATAGCCAGCTCCCCAGAAATGAACCTGCCCAC
  GGTGTCCGCGCTCAGGAAGATGGGTGTGAACCTGACACGCAGCAACAAGGAGACGGTGAA
  GCACAGCGACGTCCTGTTTCTGGCTGTGAAGCCACATATCATCCCCTTCATCCTGGATGA
  GATTGGGGCCGACGTGCAAGCCAGACACATCGTGGTCTCCTGTGCGGCTGGTGTCACCAT
  CAGCTCTGTGGAGAAGAAGCTGATGGCATTCCAGCCAGCCCCCAAAGTGATTCGCTGCAT
  GACCAACACACCTGTGGTAGTGCAGGAAGGCGCTACAGTGTACGCCACGGGCACCCATGC
  CCTGGTGGAGGATGGGCAGCTCCTGGAGCAGCTCATGAGCAGCGTGGGCTTCTGCACTGA
  GGTGGAAGAGGACCTCATCGATGCCGTCACGGNGCTCAGTGGCAGCGGGCCTGCCTATGC
  ATTCATGGCTCTGGACGCATNGGCTGATGGTGGGGTGAAGAAGGGGGTTGCCACCGCGCC
  CTGCCATCCAACTCGGNGCCCCAGCTTTGCTGGNAGCTGCNCAGATGCTGCTGGACTCGN
  AGCAGCATCCATGCCAGCTTAAGGNACATGTCTGCTCCCCTGGGGGAGCCACCATCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013328 unedited
  CGCGGCCGCAATCTAGAGTCGAGTTNTTNTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAT
  AAACTTTATTAAAGCAGTTAAACTTAGCATTAAATAACACTCTTTAAATGGTACACCTAT
  GAAGCAAGAGTTAAATATAAACCCAGTCTAATCCTGTACACTTGGGATTAATTGGGACAA
  TCTTAAGTTGCTCACTTCTTTCCCATTTACCAATTCAGAGAAAGCCCGTTTCCTGTTTTC
  TCCTCACCACTTTGCCTTGGCATCACACCAACCCTGCCTCGGGCTTCAGCTGCAGATCCT
  CCCCAGCCCCTCCTCCCAGCTGGGCTGACTCCAGTCCCAGCCCCAGTCTCCACCAACTGA
  GCAGCGTACGCAGGGTTGTGTCTGGCTTCCAGCATCTACCACCCCTTCAGAGCAACTTCC
  AACATGGGACAGGAGAGGAAGCTCGCATTGCTTGGTCTGAACAGATTTAAGGAGGTTTTA
  TCACAAGGACCTGAAAACTTCCTAAGCATGCTTTTCTCCTTGCACAGCTGAGGAGGGGCA
  ATGGTGGGAGCGGGGCAGGGGGGTGGCAGGGGCGGCAGGGGCTCTCAACTAAGGGCTCTG
  AATACAGAGGGGACAGATGCTGGCTTAATCCTTTTTGCCTCCAGGGCCAGGCTTCTTGGG
  AGGAGCTTCCTGGGCTGGAGGGGCCACTGTGAAAACTGGGGGATTCCACCTTACTCTGCC
  AAGAGGGTTTTTTAGGGGCATTGGGGAAACCTTTTTTGGCCCCCCTGGACTGGTACTCTC
  TTGTTCGATACCGAGAGCCCCACTCCTTTGTAAAACAAGACGGAACCCCCCACTTTTTAA
  AAGGGCGGGCGGGGCATGTGGGCTCCCCCGGGGGACCCACCTTCGCCTAACCCTGCTAGG
  ATGTCGCCCACCACCACACTACTCTTTGGAGTCTCTCCATAAACCCGCCCCCCCTTCTTC
  GCTCCCATTTCCCTGCCCCCCTCTTTTCCCCTACTTTTCCAACCCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013328
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013328.2, NP_037460.2
• RefSeq Size: 1708 bp
• RefSeq ORF: 963 bp
• Locus ID: 29920
• UniProt ID: Q96C36
• Cytogenetics: 1q42.12
• Domains: P5CR
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• Gene Summary: This gene belongs to the pyrroline-5-carboxylate reductase family. The encoded mitochondrial protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).