Isoleucyl tRNA synthetase (IARS) (NM_013417) Human Untagged Clone

CAT#: SC115260

IARS (untagged)-Human isoleucyl-tRNA synthetase (IARS), transcript variant long

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Isoleucyl tRNA synthetase
• Synonyms: GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_013417, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTTCAACAAGTTCCAGAAAACATAAATTTTCCTGCTGAAGAAGAGAAAATCTTGGAGTTTTGGACTG
  AATTTAATTGTTTTCAGGAATGCTTAAAGCAATCAAAACATAAACCAAAATTTACCTTCTATGATGGTCC
  TCCTTTTGCAACTGGACTGCCTCACTATGGACATATACTTGCGGGTACAATTAAAGATATAGTTACAAGA
  TATGCTCACCAGAGTGGGTTTCATGTTGACAGAAGATTTGGATGGGATTGCCATGGCTTACCTGTGGAAT
  ATGAAATTGATAAGACACTGGGAATCAGAGGACCAGAGGATGTGGCCAAAATGGGGATTACAGAGTATAA
  CAATCAGTGCCGAGCAATTGTGATGAGATATTCTGCTGAGTGGAAGTCTACTGTTAGCAGACTTGGCCGA
  TGGATTGACTTTGACAATGACTATAAAACTCTGTATCCACAATTCATGGAATCAGTCTGGTGGGTCTTCA
  AACAACTCTATGATAAAGGCCTTGTTTATAGAGGTGTGAAAGTCATGCCCTTCTCTACGGCATGTAACAC
  TCCACTTTCCAACTTCGAGTCACACCAGAATTATAAGGATGTTCAAGATCCTTCAGTATTTGTAACTTTC
  CCTTTGGAAGAAGATGAAACTGTATCTTTAGTTGCTTGGACAACCACTCCCTGGACTCTACCTAGTAACC
  TTGCTGTGTGTGTTAATCCAGAAATGCAATATGTGAAAATTAAAGATGTTGCCAGAGGACGATTACTCAT
  TTTAATGGAAGCCAGATTGTCAGCCCTCTATAAATTGGAGAGTGACTATGAGATCCTTGAAAGATTTCCT
  GGTGCCTATCTTAAAGGCAAGAAGTACAGGCCCCTGTTTGACTATTTCCTGAAGTGTAAAGAGAATGGCG
  CTTTCACTGTGCTTGTTGACAACTATGTGAAGGAAGAAGAAGGCACAGGGGTTGTCCACCAAGCTCCTTA
  CTTCGGTGCTGAGGACTATCGGGTCTGTATGGACTTTAACATTATTCGGAAAGACTCACTCCCTGTTTGC
  CCTGTGGATGCTTCAGGCTGCTTCACAACGGAGGTGACAGATTTCGCAGGACAGTATGTGAAGGATGCTG
  ACAAAAGTATCATCAGGACTTTGAAGGAACAAGGCCGACTTCTGGTTGCCACCACCTTCACTCACAGCTA
  CCCTTTTTGCTGGAGATCAGACACTCCTCTAATTTACAAAGCAGTGCCCAGCTGGTTTGTGCGAGTGGAG
  AACATGGTGGACCAGCTCCTAAGGAACAATGACCTGTGCTACTGGGTCCCAGAGTTGGTACGAGAAAAAC
  GATTTGGAAATTGGCTGAAAGATGCACGTGACTGGACAATTTCCAGAAACAGATACTGGGGCACCCCCAT
  CCCACTGTGGGTCAGCGATGACTTTGAGGAGGTGGTATGCATTGGGTCAGTGGCGGAACTTGAAGAACTG
  TCAGGAGCAAAGATCTCAGATCTCCACAGAGAGAGTGTTGACCACCTGACCATTCCTTCACGCTGTGGGA
  AGGGATCCTTGCACCGCATCTCTGAAGTGTTTGACTGTTGGTTTGAGAGTGGCAGCATGCCCTATGCTCA
  GGTTCATTACCCGTTTGAAAACAAGAGGGAGTTTGAGGATGCTTTTCCTGCAGATTTCATTGCCGAGGGC
  ATCGACCAAACCAGAGGATGGTTTTATACCCTGCTGGTGCTGGCCACGGCCCTCTTTGGACAACCGCCTT
  TCAAGAACGTAATTGTGAATGGGCTTGTCCTGGCAAGTGATGGCCAAAAAATGAGCAAACGGAAAAAGAA
  TTATCCAGATCCAGTTTCCATCATCCAGAAGTATGGTGCTGATGCCCTCAGATTATATCTGATTAACTCC
  CCTGTGGTGAGAGCAGAAAACCTCCGCTTTAAAGAAGAGGGTGTGCGGGACGTCCTTAAGGATGTACTGC
  TCCCATGGTACAATGCCTATCGCTTCTTAATCCAGAACGTTCTGAGGCTCCAGAAGGAGGAAGAAATAGA
  ATTTCTCTACAATGAGAACACGGTTAGAGAAAGCCCCAACATTACAGACCGGTGGATCCTGTCCTTCATG
  CAGTCTCTCATTGGCTTCTTTGAGACTGAAATGGCAGCTTATAGGCTTTATACTGTGGTGCCTCGCCTGG
  TCAAGTTTGTAGATATTCTGACCAATTGGTATGTTAGAATGAACCGCAGAAGATTAAAGGGTGAAAATGG
  GATGGAGGATTGTGTCATGGCCCTAGAAACCTTGTTTAGTGTTCTGCTTTCTCTTTGCAGACTTATGGCT
  CCCTACACACCTTTTCTCACTGAATTGATGTACCAGAATCTAAAGGTGCTGATTGACCCTGTTTCTGTTC
  AGGACAAGGACACACTCAGCATTCACTACCTCATGCTGCCCCGTGTTCGAGAAGAATTGATTGACAAGAA
  AACAGAGAGTGCAGTATCTCAGATGCAGTCTGTGATTGAACTTGGAAGAGTGATCAGAGACCGAAAAACT
  ATTCCCATAAAGTATCCTTTGAAAGAAATTGTGGTTATCCATCAAGATCCAGAAGCTCTTAAAGATATCA
  AGTCTTTGGAGAAGTATATCATTGAGGAACTCAATGTTCGAAAAGTTACACTGTCTACAGATAAAAACAA
  GTATGGCATTCGGCTAAGGGCAGAACCAGATCACATGGTCCTGGGGAAGCGTCTGAAGGGAGCCTTTAAG
  GCAGTGATGACGTCCATCAAGCAGTTGAGCAGTGAGGAGCTGGAGCAGTTCCAGAAGACTGGGACCATTG
  TTGTGGAAGGCCATGAATTGCACGATGAAGACATCCGCCTCATGTACACCTTTGATCAGGCCACAGGTGG
  GACTGCGCAATTTGAAGCACACTCAGATGCTCAGGCTTTGGTCCTCTTAGATGTCACTCCTGACCAGTCA
  ATGGTAGATGAAGGAATGGCTCGGGAAGTCATCAATCGCATACAGAAACTTCGCAAAAAGTGCAATCTGG
  TTCCAACTGATGAAATCACAGTGTACTATAAAGCAAAGTCTGAAGGAACATATCTGAATAGTGTTATTGA
  AAGCCACACAGAGTTCATATTTACCACCATAAAGGCTCCCTTGAAACCATATCCAGTTTCTCCATCGGAT
  AAAGTCCTTATTCAAGAAAAAACACAGTTGAAGGGATCTGAACTGGAAATTACACTCACCAGAGGATCTT
  CCCTTCCTGGTCCTGCTTGTGCATATGTCAATCTTAACATTTGTGCAAATGGCAGTGAACAAGGTGGAGT
  ATTGCTCCTGGAAAATCCAAAAGGTGACAATAGGTTGGACCTTTTAAAGCTGAAGAGTGTTGTCACTAGC
  ATTTTTGGTGTGAAAAATACAGAGCTGGCTGTCTTCCATGATGAAACAGAAATACAAAACCAAACTGACT
  TACTGAGTCTTAGTGGAAAAACACTTTGTGTGACTGCAGGATCGGCTCCCTCTCTGATCAACAGTTCTAG
  TACTCTTCTTTGTCAGTATATCAACCTACAGCTCCTGAATGCAAAGCCACAAGAGTGTTTAATGGGGACA
  GTGGGCACTCTCCTGCTTGAAAACCCACTTGGGCAGAATGGACTCACCCACCAAGGTCTTCTGTATGAAG
  CAGCCAAGGTGTTTGGCCTTCGGAGCAGGAAGCTAAAGCTGTTTCTGAATGAGACCCAAACGCAGGAAAT
  TACAGAAGACATCCCCGTGAAGACTTTGAATATGAAGACTGTGTATGTTTCTGTGTTACCAACAACAGCA
  GACTTCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013417 unedited
  TCGGCATTTTGTAATACGACTTCACTATAGGGCGGCCGCGATTCGGCACCAGCGTTGCAT
  CACCTCCGTGCGCCCGGTTGCAGCGTGGACGCCGGATGAGTTGCTTTTAGGCTTGCTGGC
  CCGCGGGGCTGTCCAGGCACGCGAGGCCCCTCAGCAACAAAATGCTTCAACAAGTTCCAG
  AAAACATAAATTTTCCTGCTGAAGAAGAGAAAATCTTGGAGTTTTGGACTGAATTTAATT
  GTTTTCAGGAATGCTTAAAGCAATCAAAACATAAACCAAAATTTACCTTCTATGATGGTC
  CTCCTTTTGCAACTGGACTGCCTCACTATGGACATATACTTGCGGGTACAATTAAAGATA
  TAGTTACAAGATATGCTCACCAGAGTGGGTTTCATGTTGACAGAAGATTTGGATGGGATT
  GCCATGGCTTACCTGTGGAATATGAAATTGATAAGACACTGGGAATCAGAGGACCAGAGG
  ATGTGGCCAAAATGGGGATTACAGAGTATAACAATCAGTGCCGAGCAATTGTGATGAGAT
  ATTCTGCTGAGTGGAAGTCTACTGTTAGCAGACTTGGCCGATGGATTGACTTTGACAATG
  ACTATAAAACTCTGTATCCACAATTCATGGAATCAGTCTGGTGGGTCTTCAAACAACTCT
  ATGATAAAGGCCTTGTTTATAGAGGTGTGAAAGTCATGCCCTTCTCTACGGCATGTAACA
  CTCCACTTTCCAACTTCGAGTCACACCAGAATTATAAAGATGTTCAAGATCCTTCAGTAT
  TTGTAACTTTCCCTTTGGAAGAAGATGAAACTGTATCTTTAGTTGCTTGGACACCACNTC
  CTGGACTCTACCTAGTACCCTGCTGTGTGTGTTATCCAGAATGCATATGTGAAAATAAAG
  ATGTGCCAAAGGACGATACTCATTTAATGGAGCCAGATGTAGCCCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013417 unedited
  CCCCCCCCTTTTACTTGNCGCGCGGCCGCTTTCTACGACCGGTCTTTTTTTTTTTTTTTT
  TTTTACACCTGAGTTTGTTTATTGTTCTGATTTACTGCAACTTAAATATTCCATCAAATT
  ATCCAGGACAATCCAGGTGGCAGACAAATATAATATGTCCATTTCATCACGAGGTCTCAA
  ACAAATTTTAAAAGGCCAGACAATGATATATATACTATGCCATTTAAATCACTTCTATCT
  TCTGTACTTAAGAACTCACGTATAGAAATAAACTGTGGGCTGAAGTAACATTGTAACCTG
  CTCCCAACATGACTGCATAGGTGTCTAAGGTTAAGTGTGAACATTACTGCGAGGTCTCAC
  GTTACTTGACTAATTCCTCTCCATTTGAATTTCAATCCTCGCACTATATTTTACACCCAC
  CTGTAAGGAACACACCTTCCGCGCGTCCATGTGTGCGCCTATGCGCATGCATGTGTACGG
  GACTCGTGTCACCCCGCGAACTCCCCCCACCACCCTCCCCCACCAATTTCACCCCCCCAC
  CTCCCTCTCTCTTCTCCCCTCCTCCTCCCCCCCCACACCCCCCTCTCCTTCTTGTCTCCT
  TTTACCACCCCCTCCATCCCCTCACCACCCCCCCCCCTCCCCGCCCACACCCTTCCCTCC
  CTACCCCCCCTCACTCCCCCACCACTCTTCCCCATCCCCCTCACCTCCTCTCCCCCACCA
  TTCTTTCCTCTTCCTTCCTCCTTCTCCTCCTCCTTCCCTCCCCTCCTTCCCCTCTGTCTC
  TTATTTCCTCCCCTCCCTCCCCCACCCACTACACCGCCTACTCCACCTCTCCCCAACGTA
  ATCCACTACTATCCGACCGTACCTCATTCTACCCTCCTCCCAATCCACGTCTAACTTCCC
  CATATTGTCACTTCCAACCCAACCACCATTATTCACCCCTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013417
• Insert Size: 3890 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013417.1, NP_038203.1
• RefSeq Size: 4508 bp
• RefSeq ORF: 3789 bp
• Locus ID: 3376
• UniProt ID: P41252
• Cytogenetics: 9q22.31
• Domains: tRNA-synt_1
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis
• Gene Summary: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found. [provided by RefSeq, Nov 2012]
  Transcript Variant: This variant (2) has an alternate splice site in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.