DAP Kinase 2 (DAPK2) (NM_014326) Human Untagged Clone

CAT#: SC115043

DAPK2 (untagged)-Human death-associated protein kinase 2 (DAPK2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DAP Kinase 2
• Synonyms: DRP-1; DRP1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014326 edited
  ATGTTCCAGGCCTCAATGAGGAGTCCAAACATGGAGCCATTCAAGCAGCAGAAGGTGGAG
  GACTTTTATGACATCGGAGAGGAGCTGGGGAGTGGCCAGTTTGCCATCGTGAAGAAGTGC
  CGGGAGAAGAGCACGGGGCTTGAGTATGCAGCCAAGTTCATCAAGAAGCGGCAGAGCCGG
  GCGAGCCGGCGCGGTGTGAGCCGGGAGGAGATCGAGCGGGAGGTGAGCATCCTGCGGCAG
  GTGCTGCACCACAATGTCATCACGCTGCACGACGTCTATGAGAACCGCACCGACGTGGTG
  CTCATCCTTGAGCTAGTGTCTGGAGGAGAGCTCTTCGATTTCCTGGCCCAGAAGGAGTCA
  CTGAGTGAGGAGGAGGCCACCAGCTTCATTAAGCAGATCCTGGATGGGGTGAACTACCTT
  CACACAAAGAAAATTGCTCACTTTGATCTCAAGCCAGAAAACATTATGTTGTTAGACAAG
  AATATTCCCATTCCACACATCAAGCTGATTGACTTTGGTCTGGCTCACGAAATAGAAGAT
  GGAGTTGAATTTAAGAATATTTTTGGGACGCCGGAATTTGTTGCTCCAGAAATTGTGAAC
  TACGAGCCCCTGGGTCTGGAGGCTGACATGTGGAGCATAGGCGTCATCACCTACATCCTC
  TTAAGTGGAGCATCCCCTTTCCTGGGAGACACGAAGCAGGAAACACTGGCAAATATCACA
  GCAGTGAGTTACGACTTTGATGAGGAATTCTTCAGCCAGACGAGCGAGCTGGCCAAGGAC
  TTTATTCGGAAGCTTCTGGTTAAAGAGACCCGGAAACGGCTCACAATCCAAGAGGCTCTC
  AGACACCCCTGGATCACGCCGGTGGACAACCAGCAAGCCATGGTGCGCAGGGAGTCTGTG
  GTCAATCTGGAGAACTTCAGGAAGCAGTATGTCCGCAGGCGGTGGAAGCTTTCCTTCAGC
  ATCGTGTCCCTGTGCAACCACCTCACCCGCTCGCTGATGAAGAAGGTGCACCTGAGGCCG
  GATGAGGACCTGAGGAACTGTGAGAGTGACACTGAGGAGGACATCGCCAGGAGGAAAGCC
  CTCCACCCACGGAGGAGGAGCAGCACCTCCTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014326 unedited
  NGGGTCAGAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGCCGCCGTGC
  CCGCCGCTCCCGCCGCGCCCGCCGCGGCCGCGACCGCGGCAGCTCAGCCTCCCGCCGATT
  GTATGTTCCAGGCCTCAATGAGGAGTCCAAACATGGAGCCATTCAAGCAGCAGAAGGTGG
  AGGACTTTTATGACATCGGAGAGGAGCTGGGGAGTGGCCAGTTTGCCATCGTGAAGAAGT
  GCCGGGAGAAGAGCACGGGGCTTGAGTATGCAGCCAAGTTCATCAAGAAGCGGCAGAGCC
  GGGCGAGCCGGCGCGGTGTGAGCCGGGAGGAGATCGAGCGGGAGGTGAGCATCCTGCGGC
  AGGTGCTGCACCACAATGTCATCACGCTGCACGACGTCTATGAGAACCGCACCGACGTGG
  TGCTCATCCTTGAGCTAGTGTCTGGAGGAGAGCTCTTCGATTTCCTGGCCCAGAAGGAGT
  CACTGAGTGAGGAGGAGGCCACCAGCTTCATTAAGCAGATCCTGGATGGGGTGAACTACC
  TTCACACAAAGAAAATTGCTCACTTTGATCTCAAGCCAGAAAACATTATGTTGTTAGACA
  AGAATATTCCCATTCCACACATCAAGCTGATTGACTNTGGTCTGGCTCACGAAATAGAAG
  ATGGAGTTGAATTTAAGAATATTTTTTGGGACGCCGGAATTTGTTGCTCCAGAAATTGTG
  AACTACGAGCCCCTGGGTCTGGNAGCTGACATGTGGAGCATANGCGTCATCACCTACATC
  CTCTTAAGTGGAGCATNCCCTTTNCTGNGAGACACGAAGCANGAAACACTGGCAATATCA
  CAGCAGTGAGTTACGACTTTGATGAAGAATNCTTCAGNCAGACGAGCGAGCTGGGCAAGG
  ACTTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014326 unedited
  TACTTTGAACCGCGGCCCGCATTTCTANGATCGAGTTTTTTTTTTTTTTTTTTTTGGCTT
  AAAAGACAGAAGTTTATTTTCTTACAATTTTTGAGGCCAGAGTCTAAGATTGAGGTGTCA
  GCAGGGTTGGTTTCCTCTGAGGCCCCCTTAGTTTGTCGATGGCTATTCCCCCTCCCACTC
  CTGGGCCTTCATGTGGTCTTTCCTGCGTGTCCTAACCTCCCCTTCTTATAAGGACACCAA
  TTATATTGCATCGGGGCCCATATCAGTGAGCTCATTTTAATTTAAGTGCCTTTCTAAAGA
  CCCCATCTCCAAATACAGTGATATTCTGTGGTACCAAGGGTTAAGACTTCAACATAGAAA
  TTTTAGGGAGACACACTCAGCTCCTAACAGCAGGAGTGGTGGCAGTGAGGTAGAGGGAGC
  ATGGGCATACTCGTCTCTGGCAACTTTTTTTTTTTTGGATATGGTCTCTCTCTGTCACCC
  AGACTGGAGTGCAGTGGCGTGATCTCAGCTCACTGCAGCCTCGACCTTCTGGGCTTAAGC
  AATGCTGTCACCTCAGCCCCTGGAGTAGCTGGGATGCCAGGTGCCTACCCCTACACCTGG
  CTAATTTTTTGTATTTTTTGTACAGACTGCGTTTTGCCATGTTGCCCAGGCTGGTCTCCA
  ACTCCTGAGTTCAAGCAATTCCCCTGCCTCGGACTGCCAAAGCGCAGGGATTATAGGCGT
  GAGCCACCACTCCCAGTCTCTGGGTGGGTTCTCTTGGACTGGACACCTTCCTGTTAAGGA
  AGCAGGGAGAAGCAGGCCCACTGCAAGCCTGCCCGGACCGTTCAGGAGGACCTCTGAGAT
  CTTCCCACTCAGCACCCACTGGGTGCCCCCCTCAGATTACCAGCCCACGGGCTAAAGGAA
  AGAGATGGGGGTACCCCTAAAGAACCCAGCTCAGGTAACCCAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014326
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014326.3, NP_055141.2
• RefSeq Size: 2628 bp
• RefSeq ORF: 1113 bp
• Locus ID: 23604
• UniProt ID: Q9UIK4
• Cytogenetics: 15q22.31
• Domains: pkinase
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Bladder cancer, Pathways in cancer
• Gene Summary: This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq, Jul 2008]