Cytochrome C (CYCS) (NM_018947) Human Untagged Clone

CAT#: SC113344

CYCS (untagged)-Human cytochrome c, somatic (CYCS), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cytochrome C
• Synonyms: CYC; HCS; THC4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113344 sequence for NM_018947 edited (data generated by NextGen Sequencing)
  ATGGGTGATGTTGAGAAAGGCAAGAAGATTTTTATTATGAAGTGTTCCCAGTGCCACACC
  GTTGAAAAGGGAGGCAAGCACAAGACTGGGCCAAATCTCCATGGTCTCTTTGGGCGGAAG
  ACAGGTCAGGCCCCTGGATACTCTTACACAGCCGCCAATAAGAACAAAGGCATCATCTGG
  GGAGAGGATACACTGATGGAGTATTTGGAGAATCCCAAGAAGTACATCCCTGGAACAAAA
  ATGATCTTTGTCGGCATTAAGAAGAAGGAAGAAAGGGCAGACTTAATAGCTTATCTCAAA
  AAAGCTACTAATGAGTAA
  
  Clone variation with respect to NM_018947.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018947 unedited
  CCGTCACATTTGTATACGATCATATAGGCGGCCGCGCAATTCGCACGAGGCCGGCTTCGG
  AGCGGGAGTGTTCGTTGTGCCAGCGACTAAAAACAGAATTAAATATGGGTGATGTTGAGA
  AAGGCAAGAAGATTTTTATTATGAAGTGTTCCCAGTGCCACACCGTTGAAAAGGGAGGCA
  AGCACAAGACTGGGCCAAATCTCCATGGTCTCTTTGGGCGGAAGACAGGTCAGGCCCCTG
  GATACTCTTACACAGCCGCCAATAAGAACAAAGGCATCATCTGGGGAGAGGATACACTGA
  TGGAGTATTTGGAGAATCCCAAGAAGTACATCCCTGGAACAAAAATGATCTTTGTCGGCA
  TTAAGAAGAAGGAAGAAAGGGCAGACTTAATAGCTTATCTCAAAAAAGCTACTAATGAGT
  AATAATTGGCCACTGCCTTATTTATTACAAAACAGAAATGTCTCATGACTTTTTTATGTG
  TACCATCCTTTAATAGATCTCATACACCAGAATTCAGATCATGAATGACTGACAGAATAT
  TTTGTTGGGCAGTCCTGATTTAAAACTAAGACTGGCTTGTGGTTAAATGAATATGTTCAG
  TTTTTGAATTTTAATAGTAACTCCAATTCAGTAAATGGTATCACTGTTTACCCCTTTTAA
  AGATATGATTAGACTTCGTTAGTAATGTTCAACTTTTCACAAAGATGGTGAGTGCCATCT
  TAAAACTTACTGGAGATTGGTTTTATATTTAGATTTATATAACTGGTTATGTGAATATAT
  TTAAATACTGGGNGAAATTGCTTCACTGTCTTAGCACCAAAGCAGAATCACCTGTGTTNT
  GGNGTCATGTTCATTTGCCTCTTAAGGCTAGGGTGCAGATAATAAGTACCAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018947 unedited
  GGGCCAGGTAAGGCACTGGGGAGGGGTCACAGGATGCCACCCGGGATCTGTTCAGGAAAC
  AGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATGATCTGT
  GGTTTTCTTTTTAATTATCTTTAGTCTTGTGATCACACATAATTTTAAAATTTGTGTATA
  TCTCCGTTACTTTAATCCTTTTAAGTTGGCAAAAGCACCATTCCCAATCAAATACACAGT
  TCTACAGTTTTGTTTCTGAATGGCTGTTTAAAGACAATCCTAAATTATAACTTAGTTTGA
  CTTAGATTGTAAAGAATTCAAGAGTGAAGTTTAACTTGCTACTATTTTAAAAGCATGTGA
  CCTTATAGATCTGTAAGATGTGAGAGGTGTTGAATAATCTTTAATATTACACATAAACCA
  CACTAAAATGCCTTTCAATAAGTAAAAGAAACCATTTTAAATACAGGGAATTATAATTAG
  ATTGGCATAGTTAAGGCCAAAACTATAGACATTGCTACCTTATTTATCTTCAACCCTTGC
  CTTTAAGAGGCAAATGAACATGAACACAAAACACAGGTGAATCTTGCTTGGTTCTAAGAC
  AGTGAAGCANTTTCCCCAGTATTTAAATATATTCACATAACCAGTTATATAAATCTAANA
  TATAAACCAATCTCCAGTAAGTTTTAAGATGCACTCACCATCTTTGTGAANAAGTGACAT
  TACTANCGAAGTCTAATCATATCTTTAAAGGGGNTAANCAGTGATACCATTTACTGATTT
  GGAGTTTACTATAAANATCANAAACTGGACATATTATTTAACCCCAGCCAGTTCTTAGTT
  TAATCAGGACTGCCCCACAAATATCTGNCAGCATTCATGATCTGAATTCTGTGTTTGAAG
  ATCATTAAAGGATGCTACCACTANAAGTCTGAACCATTTCTGTTTGTATAAAATAGGCNT
  GCCCATATACTCATTANAAGCTTTTGGAAAAGCTATTAGNCTGCCCTTCTTCTTTCTTTA
  TGCCACAGATACATTTTGTCCAGGGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018947
• Insert Size: 1330 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018947.4, NP_061820.1
• RefSeq Size: 5518 bp
• RefSeq ORF: 318 bp
• Locus ID: 54205
• UniProt ID: P99999
• Cytogenetics: 7p15.3
• Domains: cytochrome_c
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Huntington's disease, p53 signaling pathway, Parkinson's disease, Pathways in cancer, Small cell lung cancer, Viral myocarditis
• Gene Summary: This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]